Identification of RAPD markers linked to the rust (Uromyces fabae) resistance gene in pea (Pisum sativum)
- 174 Downloads
Two RAPD markers linked to gene for resistance (assayed as pustule number cm−2 leaf area) to rust [Uromyces fabae (Pers.) de Bary] in pea (Pisum sativum L.) were identified using a mapping population of 31 BC1F1 [HUVP 1 (HUVP 1 × FC 1] plants, FC 1 being the resistant parent. The analysis of genetics of rust resistance was based on the parents, F1, F2, BC1F1 and BC1F2 generations. Rust resistance in pea is of non-hypersensitive type; it appeared to be governed by a single partially dominant gene for which symbol Ruf is proposed. Further, this trait seems to be affected by some polygenes in addition to the proposed oligogene Ruf. A total of 614 decamer primers were used to survey the parental polymorphism with regard to DNA amplification by polymerase chain reaction. The primers that amplified polymorphic bands present in the resistant parent (FC 1) were used for bulked segregant analysis. Those markers that amplified consistently and differentially in the resistant and susceptible bulks were separately tested with the 31 BC1F1 individuals. Two RAPD makers, viz., SC10-82360 (primer, GCCGTGAAGT), and SCRI-711000 (primer, GTGGCGTAGT), flanking the rust resistance gene (Ruf) with a distance of 10.8 cM (0.097 rF and LOD of 5.05) and 24.5 cM (0.194 rF and a LOD of 2.72), respectively, were identified. These RAPD markers were not close enough to Ruf to allow a dependable maker-assisted selection for rust resistance. However, if the two makers flanking Ruf were used together, the effectiveness of MAS would be improved considerably.
Key wordsbulked segregant analysis linkage mapping pea rust Pisum sativum RAPD marker Uromyces fabae
Unable to display preview. Download preview PDF.
- Abusaleha, S., A.B. Pal & A. Vani, 1987. Linkage relationships between genes controlling resistance to rust and flower as well as stipule base colour in peas. Indian J Hortic Res 47: 66–69.Google Scholar
- Chand, R., C.P. Srivastava & Chanda Kushwaha, 2004. Screening technique for pea (Pisum sativum L.) genotypes against rust disease (Uromyces fabae (Pers de) Bary). Indian J Agric Sci 74: 166–167.Google Scholar
- Chand, R., C.P. Srivastava, B.D. Singh & S.B. Sarode, in press. Identification and characterization of slow rusting components in pea (Pisum sativum L.). Genet Resour Crop Evol (in press).Google Scholar
- Correa, R.X., M.R. Costa, P.I. Good-God, V.A. Ragagnin, F.G. Faleiro, M.A. Moreira & E.G. de Barros, 2000. Sequence characterized amplified regions linked to rust resistance genes in the common bean. Crop Sci 40: 804–807.Google Scholar
- Edington, B.R., P.E. Shanakan, J. Levis & F.H.J. Rajkenberg, 1994. Inheritance of partial resistance to bean rust in the common bean (Phaseolus vulgaris L.) Ann Appl Biol 125: 601–603.Google Scholar
- Haldane, J.B.S., 1919. The combination of linkage values, and the calculation of distance between the loci of linked factors. J Genet 8: 299–309.Google Scholar
- Haley, S.D., P.N. Miklas, J.R. Stavely, J. Byrum & J.D. Kelly, 1993. Identification of RAPD markers linked to a major rust resistance gene block in common bean. Theor Appl Genet 86: 505–512.Google Scholar
- Katiyar, R.P. & R.S. Ram, 1987. Genetics of rust resistance in pea. Indian J Genet 47: 46–48.Google Scholar
- Kumar, R., S.K. Mishra & B. Sharma, 2001. Genetics of rust resistance in lentil. Indian J Genet 61: 238–241.Google Scholar
- Kumar, T.B.A., K.T. Rangaswamy & K. Ravi, 1994. Assessment of tall field pea genotypes for slow rusting resistance. Legume Res 17: 79–82.Google Scholar
- Lincoln, S.E., M.J. Daly & E.S. Lander, 1993. Constructing genetic linkage maps with MAPMAKER EXP V3.0: A tutorial and reference manual. A Whitehead Institute for Biomedical Research Technical Report. http://firstname.lastname@example.org.
- Pal, A.B., H.S. Sohi & R.D. Rawal, 1979. Studies on resistance to rust (Uromyces fabae) pers. De Bary) on peas. SABRAO J 11: 101–103.Google Scholar
- Sambrook, J., E.F. Fritsch & T. Maniatis, 1989. Molecular Cloning: A Laboratory Manual. Cold Spring Harbour Laboratory Press, USA.Google Scholar
- Sanger, R.B.S. & V.K. Singh, 1994. Effect of sowing dates and pea variety on severity of rust and powdery mildew. Indian J Pulses Res 7: 8–9.Google Scholar
- Singh, R. & H. Ram, 2001. Inheritance of days to flowering and rust resistance in peas. Res Crops 2: 414–418.Google Scholar
- Singh, R.M. & C.P. Srivastava, 1985. Evaluation, classification and usefulness of pea germplasm for quantitative characters. Legume Res 8: 68–72.Google Scholar
- Singh, V.K., R.B.S. Sanger & R.N. Singh, 1996. Effect of varieties and sowing dates on disease incidence and productivity of field pea (Pisum sativum L.). Indian J Agron 4: 451–453.Google Scholar
- Stavely, J.R., 1984. Genetics of resistance to Uromyces phaseoli in a Phaseolus vulgaris line resistant to most races of the pathogen. Phytopathology 74: 339–344.Google Scholar
- Williams, K.J., A. Lichon, P. Gianquitto, J.M. Kretschmer, A. Karakousis, S. Manning, P. Langridge & H. Wallwork, 1999. Identification and amplifying of a gene conferring resistance to the spot form of net blotch (Pyrenophora teres f. maculata) in barley. Theor Appl Genet 99: 323–327.CrossRefGoogle Scholar