, Volume 142, Issue 3, pp 253–263 | Cite as

Breeding tomato for pollen tolerance to low temperatures by gametophytic selection

  • Eva Domínguez
  • Jesús Cuartero
  • Rafael Fernández-Muñoz


Haploid selection for traits related to pollen cold tolerance in tomato was performed in segregating populations derived from a Lycopersicon esculentum × L. pennellii hybrid. BC1 populations were obtained by combining normal and low temperature treatments on two stages of pollen development: pollen formation, and germination and pollen tube growth. F1 hybrids were cultivated under low and normal temperatures and their pollen was used to pollinate L. esculentum plants at low and normal temperatures. The four BC1 populations obtained were tested for the quality and quantity of pollen produced at low temperatures. The population obtained by cold treatment at both stages had a significantly improved pollen germination ability at low temperatures. The two other coldselected BC1 populations showed no differences compared with the unselected BC population. A second cycle of pollen selection, corresponding to BC2, was applied in order to test its persistence in the subsequent generations and the possibility to further improve the character. This second cycle showed no improvement although some plants retained the high pollen germination ability at low temperatures that was observed in the first cycle. Hence, gametophytic selection of some characters related with tomato pollen performance may be feasible, at least for the first cycle of selection.


gametophytic selection in vitro germination L. pennellii Lycopersicon esculentum pollen cold tolerance pollen quantity 


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Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  • Eva Domínguez
    • 1
  • Jesús Cuartero
    • 1
  • Rafael Fernández-Muñoz
    • 1
  1. 1.Estación Experimental La MayoraConsejo Superior de Investigaciones CientíficasAlgarrobo-Costa (Málaga)Spain

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