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Euphytica

, Volume 145, Issue 1–2, pp 57–66 | Cite as

New self-incompatibility alleles in apricot (Prunus armeniaca L.) revealed by stylar ribonuclease assay and S-PCR analysis

  • Júlia Halász
  • Attila Hegedüs
  • Rita Hermán
  • Éva Stefanovits-Bányai
  • Andrzej Pedryc
Article

Abstract

Apricot (Prunus armeniaca L.) shows gametophytic self-incompatibility controlled by a single locus with several allelic variants. An allele for self-compatibility (S C ) and seven alleles for self-incompatibility (S1S7) were described previously. Our experiments were carried out to ascertain whether the number of allelic variants of apricot S-locus was indeed so small. Twenty-seven apricot accessions were analysed for stylar ribonucleases by non-equilibrium pH gradient electrofocusing (NEpHGE) to determine their S-genotype. To validate the results of electrofocusing, the applicability of the S-gene-specific consensus PCR primers designed from sweet cherry sequences was tested. NEpHGE revealed 12 bands associated with distinct S-alleles in newly genotyped cultivars. Cherry consensus primers amplified 11 alleles out from 16 ones, which indicated that these primers could also recognize most of the S-RNase sequences in apricot, and provided an efficient tool to confirm or reject NEpHGE results. By combining the protein and DNA-based methods, complete or partial S-genotyping was achieved for 23 apricot accessions and nine putatively new alleles (provisionally labelled S8S16) were found. Their identity needs to be confirmed by pollination tests or S-allele sequencing. This study provides evidence that similarly to other Prunus species, the S-locus of apricot is more variable than previously believed.

Keywords

apricot consensus primers non-equilibrium pH gradient electrofocusing Prunus armeniaca L. self-incompatibility S-ribonuclease 

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Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  • Júlia Halász
    • 1
  • Attila Hegedüs
    • 2
  • Rita Hermán
    • 1
  • Éva Stefanovits-Bányai
    • 2
  • Andrzej Pedryc
    • 1
  1. 1.Department of Genetics and Plant BreedingCorvinus University of BudapestBudapestHungary
  2. 2.Department of Applied ChemistryCorvinus University of BudapestBudapestHungary

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