Advertisement

The Environmentalist

, Volume 32, Issue 2, pp 236–241 | Cite as

Magnetically treated water at 4 Hz and 2.5 mT as a modulator of cisplatin effect on cell hydration and ouabain binding of sarcoma-180 tissue

  • L. R. Yeganyan
  • R. E. Muradyan
  • F. H. Arsenyan
  • G. K. Bazikyan
  • S. N. Ayrapetyan
Article
  • 62 Downloads

Abstract

There are many data about the extremely low-frequency electromagnetic fields (ELF-EMF) therapeutic use, especially in the field of oncology. Recent data suggest that 4 Hz EMF having dehydration effect on tissues has a pronounced antitumor activity on sarcoma-180 in mice. It was shown that 4 Hz EMF have pronounced effects on physicochemical properties of water and water solution. Therefore, the aim of the present work was the comparative study of the modulation effect of ELF-EMF on cisplatin-induced changes cell hydration and number of ouabain receptors in membrane of sarcoma-180 tumor tissues. Tissue hydration was measured as wet weight/dry weight and expressed as a water content of g/g in dry weight. The number of 3H-ouabain receptors in membrane was counted by isotope scintillation counter. In conclusion, ELF-EMF can be a possible tool for stimulation of cisPt antitumor effect.

Keywords

Electromagnetic fields Cancer Cisplatin 

Notes

Acknowledgments

We thank Anna Abrahamyan for help with English editing.

References

  1. Ayrapetyan SN (1980) On the physiological significance of pump induced cell volume changes. Adv Physiolo Sci 23:67–82Google Scholar
  2. Ayrapetyan SN (1988) On the physiological significance of pump induced cell volume changes. Adv Physiolo Sci 23:67–82Google Scholar
  3. Ayrapetyan S, Akopyan K (2008) The method for inhibition of development and grow of cancer Patent No 2149 A2Google Scholar
  4. Ayrapetyan SN, Arvanov V (1979) On the mechanism of the electrogenic sodium pump dependence of membrane chemosensitivity. Comp Biochem Physiol 64a:601–604CrossRefGoogle Scholar
  5. Ayrapetyan SN, Suleymanyan MA, Saghian AA, Dadalyan SS (1984) Autoregulation of electrogenic sodium pump. Cell Mol Neurobiol 4:367–384CrossRefGoogle Scholar
  6. Ayrapetyan SN, Arvanov VL, Maginyan SB, Azatyan KV (1985) Further study of the correlation between Na-pump activity and membrane chemosensitivity. Cell Mol Neurobiol 5(3):231–243CrossRefGoogle Scholar
  7. Ayrapetyan SN, Rychkov GY, Suleymanyan MA (1988) Effect of water flow on transmembrane ionic currents in neurons of Helix pomatia and in Squid giant axon. Comp Biochem Physiol 89A:179–186CrossRefGoogle Scholar
  8. Ayrapetyan SN, Grigorian KV, Avanesian AS, Stamboltsian KV (1994) Magnetic fields alter electrical properties of solutions and their physiological effects. Bioelectromagnetics 5:133–143CrossRefGoogle Scholar
  9. Cameron IL, Sun LZ, Short N, Hardman WE, Williams CD (2005) Therapeutic electromagnetic field (TEMF) and gamma irradiation on human breast cancer xenograft growth, angiogenesis and metastasis. Cancer Cell Int 5:23CrossRefGoogle Scholar
  10. Chaplin M (2011) Water structure and science. At http://www.lsbu.ac.uk/water/
  11. Danielyan AA, Mirakyan MM, Grigoryan GY, Ayrapetian SN (1999) The static magnetic field on ouabain H3 binding by cancer tissue. Physiol Chem Phys Med NMR 31(2):139–144Google Scholar
  12. de Seze R, Tuffet S, Moreau JM, Veyret B (2000) Effects of 100mT time varying magnetic fields on the growth of tumors in mice. Bioelectromagnetics 21(2):107–111CrossRefGoogle Scholar
  13. Dvoretsky A, Shainskaya A, Ayrapetyan S (1990) Membrane structural reconstructions and ion transport system function at radiation pathology. In: Proceedings of 14th International Congress of Biochemistry, Prague, Czechoslovakia, TH:524Google Scholar
  14. Eastman A (1986) Reevaluation of interaction of cis-dichloro-(ethylenediammine) platinum(II) with DNA. Biochemistry 25:3912–3915CrossRefGoogle Scholar
  15. Fichtinger-Schepman AMJ, Van der Veer JL, Den Hartog JHJ, Lohman PHM, Reedijk J (1985) Adducts of the antitumor drug cis-diamminedichloroplatinum(II) with DNA: formation, identification, and quantitation. Biochemistry 24:707–713CrossRefGoogle Scholar
  16. Haussinger D (1996) The regulation of cell function by hydration. J Biochem 313:697–710Google Scholar
  17. Klassen VI (1982) Magnetized water systems. Chemistry 1:296–301 (in Russian)Google Scholar
  18. Lang F, Haussinger D (1993) Interaction of cell volume and cell function. Springer, HeidelbergGoogle Scholar
  19. Markov MS (1997) Clinical applications of magnetic and electromagnetic fields. In: Proceedings of IX international congress on stress montreux, Switzerland, p 56Google Scholar
  20. McManus ML and Churchwell KB (1994) In: Strange K (ed) Cellular and molecular physiology of cell volume regulation. CRC Press, Boca Raton, pp 63–77Google Scholar
  21. Mi Y, Sun C, Yao C, Li C, Mo D, Tang L, Liu H (2007) Effects of steep pulsed electric fields (SPEF) on mitochondrial transmembrane potential of human liver cancer cell. Conf Proc IEEE Eng Med Biol Soc 2007:5815–5818Google Scholar
  22. Parsegian VA (2002) Protein-water interactions. Int Rev Cytol 215:1–31. ISSN: 0074-7696Google Scholar
  23. Parsegyan VA (2002) Protein-water interaction. In: Thomas Z, Wilfred DS (eds) International review of cytology: a survey of cell biology; molecular mechanisms of water transport across biological membranes, vol 215. Academic Press, New York. pp 1–30Google Scholar
  24. Pérez RP (1998) Cellular and molecular determinants of cisplatin resistance. Eur J Cancer 34:1535–1544CrossRefGoogle Scholar
  25. Plooy ACM, Van Dijk M, Berends F, Lohman PHM (1985) Formation and repair of DNA interstrand cross-links in relation to citotoxicity and unscheduled DNA synthesis induced in control and mutant human cells treated with cis-diamminedichloroplatinum(II). Cancer Res 45:4178–4184Google Scholar
  26. Reed E, Yuspa SH, Zwelling LA, Ozols RF, Poirier MC (1986) Quantitation of cis-diamminedichloroplatinum II (cisplatin)-DNA-intrastrand adducts in testicular and ovarian cancer patients receiving cisplatin chemotherapy. J Clin Invest 77:545–550CrossRefGoogle Scholar
  27. Repacholi MH, Greenebaum B (1999) Interaction of static and extremely low frequency electric and magnetic fields with living systems: health effects and research needs. Bioelectromagnetics 20(3):133–160CrossRefGoogle Scholar
  28. Sagian A, Hunanian A, Ayrapetyan S (1997) Effect of locust poison on neuromembrane functional activity. Gen Pharmacol 29(4):587–590CrossRefGoogle Scholar
  29. Stepanyan RS, Alaverdyan ZR, Oganesyan HG, Markosyan LS, Ayrapetyan SN (2000) The effect of magnetic fields on lon mutant of escherichia coli K-12 drowth and division. Radiational Biol Radioekol 40(3):319–322Google Scholar
  30. Takahashi R, Aprison MH (1964) Acetylcholine content of discrete areas of the brain obtained by a near-freezing method. J Neurochem 11:887–898CrossRefGoogle Scholar
  31. Tofani S, Barone D, Cintorino M, de Santi MM, Ferrara A, Orlassino R, Ossola P, Peroglio F, Rolfo K, Ronchetto F (2001) Static and ELF magnetic fields induce tumor growth inhibition and apoptosis. Bioelectromagnetics 22(6):419–428CrossRefGoogle Scholar
  32. Tofani S, Cintorino M, Barone D, Berardelli M, De Santi MM, Ferrara A, Orlassino R, Ossola P, Rolfo K, Ronchetto F, Tripodi SA, Tosi P (2002) Increased mouse survival, tumor growth inhibition and decreased immunoreactive p53 after exposure to magnetic fields. Bioelectromagnetics 23:230–238CrossRefGoogle Scholar
  33. Zwelling LA, Michaels S, Schwartz H, Dobson PP, Kohn KW (1981) DNA cross-linking as an indicator of sensitivity and resistance of mouse L1210 leukemia to cis-diamminedichloroplatinum(II) and l-phenylalanine mustard. Cancer Res 41:640–649Google Scholar

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • L. R. Yeganyan
    • 1
  • R. E. Muradyan
    • 2
  • F. H. Arsenyan
    • 2
  • G. K. Bazikyan
    • 3
  • S. N. Ayrapetyan
    • 1
  1. 1.Life Sciences International Postgraduate Educational CenterYerevanArmenia
  2. 2.NAS RA the Scientific Technological Center of Organic and Pharmaceutical Chemistry SNPOYerevanArmenia
  3. 3.National Center of OncologyYerevanArmenia

Personalised recommendations