Monitoring the effects of wastewater treatment strategies

  • J. A. de-la-Ossa-Carretero
  • Y. Del-Pilar-Ruso
  • F. Giménez-Casalduero
  • J. L. Sánchez-Lizaso


Wastewater disposal in coastal waters causes widespread environmental problems. Secondary treatment is expected to reduce the adverse effects of insufficiently treated wastewater. The environmental impact of sewage disposal via 18 wastewater treatment plants was analysed using the benthic opportunistic polychaetes and amphipods (BOPA) index. In previous studies this index proved to be an effective tool for monitoring sewage pollution. The impact of these discharges was highly related to treatment level, which ranged from pre-treatment to biological, as well as to flow rates and outfall position. Locations affected by pre-treated wastewater showed environmental degradation, especially marked near outfalls with higher flow rates. At most locations, biologically treated wastewater did not cause a significant impact and an improvement in ecological integrity was detected after this secondary treatment had been implemented. The impact of discharge was highly related to chemical oxygen demand (COD), suspended solids and nutrient concentrations, which are all lower in biologically treated wastewater. A ‘moderate’ ecological status was observed not only near sewage outfalls with high wastewater flow rates (>1,500,000 m3/month) with a COD over 200 mg/l but also near those with lower flow rates but with a COD over 400 mg/l. To reduce the impact of sewage disposal, it is necessary to carry out adequate treatment, have site outfalls deep enough, and implement water recycling.


Wastewater treatment Mediterranean Sewage Benthos BOPA index Pre-treatment Biological treatment 



We gratefully acknowledge CONSOMAR S.A. and Entitat de Sanejament d’Aigües for their financial contribution to this project. We also thank the staff of the Department of Marine Sciences and Applied Biology of University of Alicante, especially Y. Mugica, M. Diaz-Valdes and C. Celdrán for their invaluable collaboration. Thanks to Guido Jones for revising the English text.

Compliance with ethical standards

This article complies with editorial and publication policies as well as ethical standards of environmental monitoring and assessment. All procedures performed were in accordance with the ethical standards of the University of Alicante. Informed consent was obtained from all individual participants included in the study.

Conflict of interest

The authors declare that they have no conflicts of interest, and funding agencies are informed.


  1. Borja, A., Muxika, I., & Franco, J. (2006). Long-term recovery of soft-bottom benthos following urban and industrial sewage treatment in the Nervión estuary (southern Bay of Biscay). Marine Ecology Progress Series, 313, 43–55.CrossRefGoogle Scholar
  2. Cardell, M. J., Sardá, R., & Romero, J. (1999). Spatial changes in sublittoral soft-bottom polychaete assemblages due to river inputs and sewage discharges. Acta Oecologica, 20, 343–351.CrossRefGoogle Scholar
  3. Chainho, P., Costa, J. L., Chaves, M. L., Dauer, D. M., & Costa, M. J. (2007). Influence of seasonal variability in benthic invertebrate community structure on the use of biotic indices to assess the ecological status of a Portuguese estuary. Marine Pollution Bulletin, 54, 1586–1597.CrossRefGoogle Scholar
  4. Cloern, J. E. (2001). Our evolving conceptual model of the coastal eutrophication problem. Marine Ecology Progress Series, 210, 223–253.CrossRefGoogle Scholar
  5. Dauvin, J. C., & Ruellet, T. (2007). Polychaete/amphipod ratio revisited. Marine Pollution Bulletin, 55, 215–224.CrossRefGoogle Scholar
  6. Dauvin, J. C., Ruellet, T., Desroy, N., & Janson, A. L. (2007). The ecology quality status of the Bay of Seine and the Seine estuary: use of biotic indices. Marine Pollution Bulletin, 55, 241–257.CrossRefGoogle Scholar
  7. De-la-Ossa-Carretero, J. A., & Dauvin, J. C. (2010). A comparison of two biotic indices, AMBI and BOPA/BO2A, for assessing the ecological quality status (EcoQS) of benthic macro-invertebrates. Transitional Water Bulletin, 4, 12–24.Google Scholar
  8. De-la-Ossa-Carretero, J. A., Del-Pilar-Ruso, Y., Giménez-Casalduero, F., & Sánchez-Lizaso, J. L. (2009). Testing BOPA index in sewage affected soft-bottom communities in the north-western Mediterranean. Marine Pollution Bulletin, 58, 332–340.CrossRefGoogle Scholar
  9. De-la-Ossa-Carretero, J. A., Simboura, N., Del-Pilar-Ruso, Y., Pancucci-Papadopoulou, M. A., Giménez-Casalduero, F., & Sánchez-Lizaso, J. L. (2012). A methodology for applying taxonomic sufficiency and benthic biotic indices in two Mediterranean areas. Ecological Indicators, 23, 232–241.CrossRefGoogle Scholar
  10. Del-Pilar-Ruso, Y., de-la-Ossa-Carretero, J. A., Giménez-Casalduero, F., & Sánchez-Lizaso, J. L. (2010). Sewage treatment level and flow rates affect polychaete assemblages. Marine Pollution Bulletin, 60, 1930–1938.CrossRefGoogle Scholar
  11. Development Core Team, R. (2009). R: a language and environment for statistical computing. Vienna, Austria: R Foundation for Statistical Computing. Scholar
  12. Diaz, R. J., & Rosenberg, R. (1995). Spreading dead zones and consequences for marine ecosystems. Science, 321, 926–929.CrossRefGoogle Scholar
  13. Díaz, R. J., Solan, M., & Valente, R. M. (2004). A review of approaches for classifying benthic habitats and evaluating habitat quality. Journal of Environmental Management, 73, 165–181.CrossRefGoogle Scholar
  14. Echavarri-Erasun, B., Juanes, J. A., Puente, A., & Revilla, J. A. (2010). Coastal outfalls, a sustainable alternative for improving water quality in north-east Atlantic estuaries. Journal of Environmental Monitoring, 12, 1737–1746.CrossRefGoogle Scholar
  15. Ganesh, T., Rakhesh, M., Raman, A. V., Nanduri, S., Moore, S., & Rajanna, B. (2014). Macrobenthos response to sewage pollution in a tropical inshore area. Environmental Monitoring and Assessment, 186, 3553–3566.CrossRefGoogle Scholar
  16. Gray, J. S., Aschan, M., Carr, M. R., Clarke, K. R., Green, R. H., Pearson, T. H., Rosemberg, R., & Warwick, R. M. (1988). Analysis of community attributes of the benthic macrofauna of Frierfjord/Langesundfjord and in a mesocosm experiment. Marine Ecology Progress Series, 46, 151–165.CrossRefGoogle Scholar
  17. Gray, J. S., Wu, R. S., & Or, Y. Y. (2002). Effects of hypoxia and organic enrichment on the coastal marine environment. Marine Ecology Progress Series, 238, 249–279.CrossRefGoogle Scholar
  18. Holme, N. A., & McIntyre, A. D. (1984). Methods for the study of marine benthos (2nd ed.). London: Blackwell.Google Scholar
  19. Islam, M. S., & Tanaka, M. (2004). Impacts of pollution on coastal and marine ecosystems including coastal and marine fisheries and approach for management: a review and synthesis. Marine Pollution Bulletin, 48, 624–649.CrossRefGoogle Scholar
  20. Koop, K., & Hutchins, P. (1996). Disposal of sewage to the ocean: a sustainable solution? Marine Pollution Bulletin, 33, 121–123.CrossRefGoogle Scholar
  21. Liu, S., Lou, S., Kuang, C., Huang, W., Chen, W., Zhang, J., & Zhong, G. (2011). Water quality assessment by pollution-index method in the coastal waters of Hebei Province in western Bohai Sea, China. Marine Pollution Bulletin, 62, 2220–2229.CrossRefGoogle Scholar
  22. Moon, H. B., Yoon, S. P., Jung, R. H., & Choi, M. (2008). Wastewater treatment plants (WWTPs) as a source of sediment contamination by toxic organic pollutants and fecal sterols in a semi-enclosed bay in Korea. Chemosphere, 73, 880–889.CrossRefGoogle Scholar
  23. Muxika, I., Borja, A., & Bonne, W. (2005). The suitability of the marine biotic index (AMBI) to new impact sources along European coasts. Ecological Indicators, 5, 19–31.CrossRefGoogle Scholar
  24. Rakocinski, C. F., Brown, S. S., Gaston, G. R., Heard, R. W., Walker, W. W., & Summers, J. K. (1997). Macrobenthic responses to natural and contaminant-related gradients in northern Gulf of Mexico estuaries. Ecological Applications, 7, 1278–1298.CrossRefGoogle Scholar
  25. Sandrini-Neto, L. & Camargo, M. G. (2011). GAD: analysis of variance from general principles. R package version 1.1.1. Available at
  26. Shuai, X., Bailey-Brock, J. H., & Lin, D. T. (2014). Spatio-temporal changes in trophic categories of infaunal polychaetes near the four wastewater ocean outfalls on Oahu, Hawaii. Water Research, 58, 38–49.CrossRefGoogle Scholar
  27. Simboura, N., Zenetos, A., & Pancucci-Papadopoulou, M. A. (2014). Benthic community indicators over a long period of monitoring (2000–2012) of the Saronikos Gulf, Greece, Eastern Mediterranean. Environment Monitoring and Assessment, 186, 3809–3821.CrossRefGoogle Scholar
  28. Stamou, A. I., & Kamizoulis, G. (2009). Estimation of the effect of the degree of sewage treatment on the status of pollution along the coastline of the Mediterranean Sea using broad scale modelling. Journal of Environmental Management, 90, 931–936.CrossRefGoogle Scholar
  29. UWWTD. (1991). Council Directive 91/271/EEC of 21 May 1991 concerning urban wastewater treatment.Google Scholar
  30. Wilson, J. G., & Jeffrey, D. W. (1994). Benthic biological pollution indices in estuaries. In K. J. M. Kramer (Ed.), Biomonitoring of coastal water and estuaries (pp. 311–327). Baton Rouge, USA: CRC Press.Google Scholar
  31. Xu, J., Lee, J. H. W., Yin, K., Liu, H., & Harrison, P. J. (2011). Environmental response to sewage treatment strategies: Hong Kong’s experience in long term water quality monitoring. Marine Pollution Bulletin, 62, 2275–2287.CrossRefGoogle Scholar
  32. Zarzo Martínez, D. (2008). Desarrollo, implantación y tecnología de las EDARs. Madrid, Spain: Actas curso. Internacional Faculty for Executives. IFAES.Google Scholar

Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  • J. A. de-la-Ossa-Carretero
    • 1
  • Y. Del-Pilar-Ruso
    • 1
  • F. Giménez-Casalduero
    • 1
  • J. L. Sánchez-Lizaso
    • 1
  1. 1.Department of Marine Sciences and Applied BiologyUniversity of AlicanteAlicanteSpain

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