Irrigation waters and pipe-based biofilms as sources for antibiotic-resistant bacteria
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The presence of antibiotic-resistant bacteria in environmental surface waters has gained recent attention. Wastewater and drinking water distribution systems are known to disseminate antibiotic-resistant bacteria, with the biofilms that form on the inner-surfaces of the pipeline as a hot spot for proliferation and gene exchange. Pipe-based irrigation systems that utilize surface waters may contribute to the dissemination of antibiotic-resistant bacteria in a similar manner. We conducted irrigation events at a perennial stream on a weekly basis for 1 month, and the concentrations of total heterotrophic bacteria, total coliforms, and fecal coliforms, as well as the concentrations of these bacterial groups that were resistant to ampicillin and tetracycline, were monitored at the intake water. Prior to each of the latter three events, residual pipe water was sampled and 6-in. sections of pipeline (coupons) were detached from the system, and biofilm from the inner-wall was removed and analyzed for total protein content and the above bacteria. Isolates of biofilm-associated bacteria were screened for resistance to a panel of seven antibiotics, representing five antibiotic classes. All of the monitored bacteria grew substantially in the residual water between irrigation events, and the biomass of the biofilm steadily increased from week to week. The percentages of biofilm-associated isolates that were resistant to antibiotics on the panel sometimes increased between events. Multiple-drug resistance was observed for all bacterial groups, most often for fecal coliforms, and the distributions of the numbers of antibiotics that the total coliforms and fecal coliforms were resistant to were subject to change from week to week. Results from this study highlight irrigation waters as a potential source for antibiotic-resistant bacteria, which can subsequently become incorporated into and proliferate within irrigation pipe-based biofilms.
KeywordsAntibiotic resistance Indicator bacteria Irrigation water Biofilm Pipes
- Armstrong, J. L., Shigeno, D. S., Calomiris, J. J., & Seidler, R. J. (1981). Antibiotic-resistant bacteria in drinking water. Applied and Environmental Microbiology, 42(2), 277–283.Google Scholar
- Brettar, I., & Hofle, M. G. (1992). Influence of ecosystematic factors on survival of Escherichia coli after large-scale release into lake water mesocosms. Applied and Environmental Microbiology, 58(7), 2201–2210.Google Scholar
- Czekalski, N., Berthold, T., Caucci, S., Egli, A., & Bürgmann, H. (2012). Increased levels of multiresistant bacteria and resistance genes after wastewater treatment and their dissemination into Lake Geneva, Switzerland. Frontiers in Microbiology, 3, 106. doi: 10.3389/fmicb.2012.00106.CrossRefGoogle Scholar
- De Lancy Pulcini, E. (2001). Bacterial biofilms: a review of current research. Néphrologie, 22(8), 439–441.Google Scholar
- Devarajan, N., Laffite, A., Graham, N. D., Meijer, M., Prabakar, K., Mubedi, J. I., Elongo, V., Mpiana, P. T., Ibelings, B. W., Wildi, W., & Pote, J. (2015). Accumulation of clinically relevant antibiotic resistant genes, bacterial load, and metals from freshwater lake sediments in Central Europe. Environmental Science and Technology, 49, 6528–6537.CrossRefGoogle Scholar
- Hammer, Ø., Harper, D. A. T., & Ryan, P. D. (2001). PAST: paleontological statistics soft-ware package for education and data analysis. Palaeontologia Electronica, 4(1), 9. http://palaeo-electronica.org/20011/past/issue101.htm.Google Scholar
- Hausner, M., & Wuertz, S. (1999). High rates of conjugation in bacterial biofilms as determined by quantitative in situ analysis. Applied and Environmental Microbiology, 65(8), 3710–3713.Google Scholar
- Lebkowska, M. (2009). Antibiotic resistant bacteria in drinking water. Ochrona Srodowiska, 31(2), 11–15.Google Scholar
- Li, X., Watanabe, N., Xiao, C., Harter, T., McCowan, B., Liu, Y., & Atwill, E.R. (2013). Antibiotic-resistant E. coli in surface water and groundwater in dairy operations in Northern California. Environmental Monit Assess. DOI 10.1007/s10661-013-3454-2.
- Martinez, J. L. (2009). Environmental pollution by antibiotics and by antibiotic resistance determinants. Environmental Pollution (Barking, Essex: 1987), 157(11), 2893–902.Google Scholar
- McCambridge, J., & McMeekin, T. A. (1980). Relative effects of bacterial and protozoan predators on survival of Escherichia coli in estuarine water samples. Applied and Environmental Microbiology, 40(5), 907–911.Google Scholar
- Pachepsky, Y., Shelton, D. R., Mclain, J. E. T., Patel, J., & Mandrell, R. E. (2011). Irrigation waters as a source of pathogenic microorganisms in produce: a review. Advances in agronomy (1st ed., Vol. 113, pp. 73-137). Elsevier Inc. doi: 10.1016/B978-0-12-386473-4.00007-5
- Rice, E. W., Messer, J. W., Johnson, C. H., & Reasoner, D. J. (1995). Occurrence of high-level aminoglycoside resistance in environmental isolates of enterococci. Applied and Environmental Microbiology, 61(1), 374–376.Google Scholar
- Sadovski, A. Y., Fattal, B., Goldberg, D., Katzenelson, E., & Shuval, H. I. (1978). High levels of microbial contamination of vegetables irrigated with wastewater by the drip method. Applied and Environmental Microbiology, 36, 824–830.Google Scholar
- USDA-ARS. (2014). NARMS—National Antimicrobial Resistance Monitoring System Animal Isolates. <http://www.ars.usda.gov/News/docs.htm?docid=6750&page=3>.
- Wellington, E. M. H., Boxall, A. B., Cross, P., Feil, E. J., Gaze, W. H., Hawkey, P. M., Johnson-Rollins, A. S., Jones, D. L., Lee, N. M., Otten, W., Thomas, C. M., & Williams, A. P. (2013). The role of the natural environment in the emergence of antibiotic resistance in gram-negative bacteria. Lancet Infectious Diseases, 13(2), 155–165. doi: 10.1016/S1473-3099(12)70317-1.CrossRefGoogle Scholar
- Yomoda, S., Okubo, T., Takahashi, A., Murakami, M., & Iyobe, S. (2003). Presence of Pseudomonas putida strains harboring plasmids bearing the Metallo-β-Lactamase gene bla IMP in a hospital in Japan. Journal of Clinical Microbiology, 41(9), 4246–4251. doi: 10.1128/JCM.41.9.4246.CrossRefGoogle Scholar