Abstract
Toxicological evaluation of Clarias gariepinus from bitumen-polluted River Oluwa, Nigeria, was carried out in furtherance of studies on the environmental impacts of the bitumen exploration in Ondo State, Nigeria. Samples were taken from three different (two polluted and one as control) sites. The effect of changes in monthly seasonal flow rate was assessed for the sites of study. Blood plasma clinical–chemical parameters (BCCPs) and histological changes/lesions in various organs were evaluated as markers of pollution in the fish blood using standard methods. The result of the physicochemical properties of water from the sampling points revealed some of the values conforming to approved standards while others showed deviation. Significant differences were found in the blood and histological endpoints between the control and the polluted sites as well as between the two seasons evaluated across the sites. The public health implications of consuming this fish are fully discussed.
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References
Adeyemo, O. K. (2005). Haematological and histopathological effects of cassava mill effluent in Clarias gariepinus. African Journal of Biomedical Research, 8, 179–83.
Atamanalp, M., & Yanik, T. (2003). Alterations in hematological parameters of rainbow trout (Oncorhynchus mykiss) exposed to mancozeb. Turkish Journal of Veterinary and Animal Science, 27, 1213–1217.
Ates, B., Orun, I., Talas, Z. S., Durmaz, G., & Yilmaz, I. (2008). Effects of sodium selenite on some bio- chemical and hematological parameters of rainbow trout (Oncorhynchus mykiss Walbaum, 1792) exposed to Pb2+ and Cu2+. Fish Physiology and Biochemistry, 34, 53–9.
Ayandiran, T. A., Ayandele, A. A., Dahunsi, S. O., & Ajala, O. O. (2014). Microbial assessment and prevalence of antibiotic resistance in polluted Oluwa River, Nigeria. Egyptian Journal of Aquatic Research, 40, 291–299.
Bedii, C., & Kenan, E. (2005). The effects of cadmium on levels of glucose in serum and glycogen reserves in the liver and muscle tissues of Cyprinus carpio (L., 1758). Turkish Journal of Veterinary and Animal Science, 29, 113–117.
Camargo, M. M., & Martinez, C. B. (2007). Histopathology of gills, kidney and liver of a Neotropical fish caged in an urban stream. Ichthyology, 5, 327–336.
Chapman, D. (1996). Water quality assessment: a guide to the use of Biota, sediments and water in environmental monitoring (2nd ed.) London, UK: Chapman and Hall.
Chen, Q., Zhang, R., Lia, W., Niu, Y., Guo, H., Liuc, X., Hou, Y., & Zhao, L. (2013). The protective effect of grape seed procyanidin extract against cadmium-induced renal oxidative damage in mice. Environmental Toxicology and Pharmacology, 36, 759–768.
Dacie, J. V., & Lewis, S. M. (1975). Measurement and calculation of size of red cells, practical haematology (5th ed., pp. 40–43). London: Churchill Livingstone.
Dahunsi, S. O., Oranusi, S. U., & Ishola, R. O. (2011). Biochemical profile of Clarias gariepinus exposed to sub-lethal concentrations of chemical additives effluent. International Journal of Research in Environmental Science and Technology, 1(4), 52–58.
Dahunsi, S. O., Oranusi, S. U., & Ishola, R. O. (2012). Differential bioaccumulation of heavy metals in selected biomarkers of Clarias gariepinus (Burchell, 1822) exposed to chemical additives effluent. Journal of Research in Environmental Science and Toxicology, 1(5), 100–106.
Dahunsi, S. O., Owamah, H. I., Ayandiran, T. A., & Oranusi, U. S. (2014). Drinking water quality and public health of selected communities in South Western Nigeria. Water Quality Exposure and Health, 6, 143–153.
Das, B. K., & Mukherjee, S. C. (2000). Chronic toxic effect of quinalphos on some biochemical parameters in Labeo robita (Ham). Toxicology Letters, 114, 11–18.
Fagbenro. O., Akinduyite, I., (2011). Acute toxicity of aqueous Morinda lucida leaf extracts to Nile tilapia, Oreochromis niloticus (Linnaeus 1857). Proceedings of the Ninth International Symposium on Tilapia in Aquaculture. Shanghai, China. April 22nd-24th, 2011. 52–59
Fanta, E., Rios, F.S., Roma˜o, S., Vianna, A.C.C., Freiberger, S., (2003). Histopathology of the fish Corydoras paleatus contaminated with sublethal levels of organophosphorus in water and food. Ecotoxicology and Environmental Safety, 54, 119–130
Honggang, Z., Baoshan, C., Rang, X., & Hui, Z. (2010). Heavy metals in water, soils and plants in riparian wetlands in the Pearl River Estuary, South China. Proceedings in Environmental Science, 2, 1344–1354.
Isitor, G.N., Rao, S., Nayak, S.B., Sundaram, V., (2012). Autofluorescent vesicular structures in hematoxylin and eosin stained duodenal mucosa of the domestic cat. West Indies Veterinary Journal, 9
Jastrzebska, E. B., & Protasowickiz, M. (2005). Effects of cadmium and nickel exposure on hematological parameters of common carp, Cyprinus carpio L. Acta Ichthyology Piscat, 35(1), 29–38.
Kavitha, C., Malarvizhi, A., Senthil, K. S., & Ramesh, M. (2010). Toxicological effects of arsenate exposure on haematological, biochemical and liver transaminase activity in an Indian major carp, Catla catla. Food and Chemical Toxicology, 48(28), 48–54.
Kavitha, C., Mathan, R., Satyanarayanan, S.K., (2011). Toxicity of Moringa oleifera seed extract on some haematological and biochemical profiles in a freshwater fish, Cyprinus carpio. Experimental and Toxicologic Pathology doi:10.1016/jetp.2011. 01.001
Keskin, Y., Raskaya, R., Ozyaral, O., Yurdun, T., Luleci, N. E., & Hayran, O. (2007). Cadmium, lead, mercury and copper in fish from the Marmara Sea, Turkey. Bulletin of Environmental Contamination and Toxicology, 78, 258–261.
Kreutz, L. C., Barcellos, L. J. G., de Faria Valle, S., de Oliveira Silva, T., Anziliero, D., dos Santos, E. D., Pivato, M., & Zanatta, R. (2011). Altered hematological and immunological parameters in silver catfish (Rhamdia quelen) following short term exposure to sublethal concentration of glyphosate. Fish and Shellfish Immunology, 30, 51–57.
Kubrak, O. I., Atamaniuk, T. M., Storey, K. B., & Lushchak, V. I. (2013). Goldfish can recover after short-term exposure to 2,4-dichlorophenoxyacetate: use of blood parameters as vital biomarkers. Comparative Biochemistry and Physiology Part C, 157, 259–265.
Lezcano, N., Sedan, D., Lucotti, I., Giannuzzi, L., Vittone, L., Andrinolo, D., & Mundiña-Weilenmann, C. (2012). Subchronic microcystin-LR exposure increased hepatic apoptosis and induced compensatory mechanisms in mice. Journal of Biochemistry and Molecular Toxicology, 26(4), 131–138.
Li, Z. H., Velisek, J., Zlabek, V., Grabic, R., Machova, J., Kolarova, J., & Randak, T. (2010). Hepatic antioxidant status and hematological parameters in rainbow trout, Oncorhynchus mykiss, after chronic exposure to carbamazepine. Chemico-Biological Interactions, 183, 98–104.
Li, Z. H., Velisek, J., Grabic, R., Li, P., Kolarova, J., & Randak, T. (2011a). Use of hematological and plasma biochemical parameters to assess the chronic effects of a fungicide propiconazole on a freshwater teleost. Chemosphere, 83, 572–578.
Li, Z. H., Velisek, J., Zlabek, V., Grabic, R., Machova, J., Kolarova, J., Ping, L., & Randak, T. (2011b). Chronic toxicity of verapamil on juvenile rainbow trout (Oncorhynchus mykiss): effects on morphological indices, hematological parameters and antioxidant responses. Journal of Hazardous Materials, 185, 870–880.
Nigerian Industrial Standards (2007). Nigerian Standards for drinking water quality. NIS 554: 2007, ICS 13.060.20. Approved by the Standard Organization of Nigeria (SON) Governing Council, pp 14–18
Olajire, A. A., Alade, A. O., Adeniyi, A. A., & Olabemiwo, O. M. J. (2007). Distribution of polycyclic aromatic hydrocarbons in surface soils and water from the vicinity of Agbabu bitumen field of South Western Nigeria. Environmental Science and Health Part A, 42, 1043.
Olufayo, M. O. (2009). Hematological characteristics of Clarias gariepinus (Burchell 1822) juveniles exposed to Derris elliptica root powder. African Journal of Food, Agriculture and Nutritional Science, 9(3), 920–33.
Olurin, K. B., & Aderibigbe, K. (2006). Length-weight relationship and condition factor of pond reared juvenile Oreochromis niloticus. World Journal of Zoology, 1(21), 82–85.
Osman, A. G. M., Koutb, M., & Sayed, A. E. D. H. (2010). Use of hematological parameters to assess the efficiency of quince (Cydonia oblonga Miller) leaf extract in alleviation of the effect of ultraviolet—a radiation on African catfish Clarias gariepinus (Burchell, 1822). Journal of Photochemistry, Photobiology B Biology, 99, 1–8.
Pimpão, C. T., Zampronio, A. R., & Silva de Assis, H. C. (2007). Effects of deltamethrin on hematological parameters and enzymatic activity in Ancistrus multispinis (Pisces, Teleostei). Pesticides Biochemistry and Physiology, 88, 122–127.
Prusty, A. K., Kohli, M. P. S., Sahu, N. P., Pal, A. K., Saharan, N., Mohapatra, S., & Gupta, S. K. (2011). Effect of short term exposure of fenvalerate on biochemical and haematological responses in Labeo rohita (Hamilton) fingerlings. Pesticides Biochemistry and Physiology, 100, 124–129.
Ptashynski, M. D., Pedlar, R. M., Evans, R. E., Baron, C. L., & Klaverkamp, J. F. (2002). Toxicology of rats chronically treated with an organophosphate. Ecotoxicology and Environmental Safety, 66, 447–450.
Renugadevi, J., & Prabu, S. M. (2010). Cadmium-induced hepatotoxicity in rats and the protective effect of naringenin. Experimental Toxicology and Pathology, 62(2), 171–181.
Richards, M. P., & Proszkowiec-Weglarz, M. (2007). Mechanisms regulating feed intake, energy expenditure, and body weight in poultry. Poultry Science, 86, 1478–1490.
Saravanan, M., Karthika, S., Malarvizhi, A., & Ramesh, M. (2011). Ecotoxicological impacts of clofibric acid and diclofenac in common carp (Cyprinus carpio) fingerlings: hematological, biochemical, ionoregulatory and enzymological responses. Journal of Hazardous Materials, 195, 188–194.
Simonato, J. D., Guedes, C. L. B., & Martinez, C. B. R. (2008). Biochemical, physiological, and histological changes in the neotropical fish Prochilodus lineatus exposed to diesel oil. Ecotoxicology and Environmental Safety, 69, 112–120.
Sobecka, E. (2001). Changes in the iron level in the organs and tissues of wels catfish, Silurus glanis L. caused by nickel. Acta Ichthyology Piscat, 31(2), 127–43.
Sonne, C., Dietz, R., Kirkegaard, M., Letcher, R. J., Shahmiri, S., Andersen, S., Møller, P., Olsen, A. K., & Jensen, A. L. (2008). Effects of organohalogen pollutants on haematological and urine clinical–chemical parameters in Greenland sledge dogs (Canis familiaris). Ecotoxicology and Environmental Safety, 69, 381–390.
Sonne, C., Bustnes, J. O., Herzke, D., Jaspers, V. L. B., Covaci, A., Halley, D. J., Minagawa, M., Moum, T., Eulaers, I., Eens, M., Ims, R. A., Hanssen, S. A., Erikstad, K. E., Johnsen, T., Schnug, L., & Jensen, A. L. (2010). Relationships between organohalogen contaminants and blood plasma clinical–chemical parameters in chicks of three raptor species from Northern Norway. Ecotoxicology and Environmental Safety, 73, 7–17.
Sonne, C., Bustnes, J. O., Herzke, D., Jaspers, V. L. B., Covaci, A., Eulaers, I., Halley, D. J., Moum, T., Ballesteros, M., Eens, M., Ims, R. A., Hanssen, S. A., Erikstad, K. E., Johnsen, T. V., Rige´t, F. F., Jensen, A. L., & Kjelgaard-Hansen, M. (2012). Blood plasma clinical–chemical parameters as biomarker endpoints for organohalogen contaminant exposure in Norwegian raptor nestlings. Ecotoxicology and Environmental Safety, 80, 76–83.
Sonne, C., Rige´t, F. F., Leat, E. H. K., Bourgeon, S., Borg, K., Strøm, H., Hanssen, S. A., Gabrielsen, G. W., Petersen, A., Olafsdottir, K., Magnusdottir, E., Bustnes, J. O., Furness, R. W., & Kjelgaard-Hansen, M. (2013). Organohalogen contaminants and blood plasma clinical–chemical parameters in three colonies of North Atlantic Great skua (Stercorarius skua). Ecotoxicology and Environmental Safety, 92, 245–251.
Soufy, M. K., Soliman, E. M., El-manakhly, A. U., & Gafar, U. (2007). Some biochemical and pathological investigations on monosex tilapia following chronic exposure to carbofuran pesticide. Global Veterinary Journal, 1, 45–52.
Thophon, S., Kruatrachue, M., Upatham, E. S., Pokethitiyook, P., Sahaphong, S., & Jaritkhuan, S. (2003). Histopathological alterations of white seabass, Lates calcarifer, in acute and subchronic cadmium exposure. Environmental Pollution, 121, 307–320.
van Dyk, J. C., Pieterse, G. M., & van Vuren, J. H. J. (2007). Histological changes in the liver of Oreochromis mossambicus (Cichlidae) after exposure to cadmium and zinc. Ecotoxicology and Environmental Safety, 66, 432–440.
Vani, T., Saharan, N., Mukherjee, S. C., Ranjan, R., Kumar, R., & Brahmchari, R. K. (2011). Deltamethrin induced alterations of hematological and biochemical parameters in fingerlings of Catla catla (Ham.) and their amelioration by dietary supplement of vitamin C. Pesticides Biochemistry and Physiology, 101, 16–20.
Vasylkiv, O. Y., Kubrak, O. I., Storey, K. B., & Lushchak, V. I. (2010). Cytotoxicity of chromium ions may be connected with induction of oxidative stress. Chemosphere, 80, 1044–1049.
Vasylkiv, O. Y., Kubrak, O. I., Storey, K. B., & Lushchak, V. I. (2011). Catalase activity as a potential vital biomarker of fish intoxication by the herbicide aminotriazole. Pesticides Biochemistry and Physiology, 101, 1–5.
Wepener, V., Van Vuren, J. H. J., & Du Preez, H. H. (1992a). The effect of iron and manganese at an acidic pH on the hematology of the banded tilapia (Tilapia sparrmanii, Smith). Bulletin of Environmental Contamination and Toxicology, 49, 613–619.
Wepener, V., Van Vuren, J. H., & Preez, H. H. (1992b). The effect of hexavalent chromium at different pH values on the haematology of Tilapia sparmani (Cichlidae). Comparative Biochemistry and Physiology, 101, 375–381.
Wintrobe, M. M. (1978). Clinical haematology (p. 488). London: Henry Kimpton.
World Health Organization (2011). Guidelines on drinking water quality, 4th Edition. NLM Classification: WA 675 (pp 307–433). Geneva, Switzerland: World Health Organization.
Yoon S, Batt SD, Lee W, Jeong SY, Baeg JO, Lee CW (2009). Separation and characterization of bitumen from Athabaca oil sand. Korean Journal of Chemical Engineering, 26, 64–71.
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The authors appreciate the efforts of the faculty and staff of the Department of Pure and Applied Biology, LAUTECH, Nigeria, for making this work a success. Special thanks to our mentor, Professor Fawole OO for guiding us well.
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Ayandiran, T.A., Dahunsi, S.O. Toxicological assessment of fish (Clarias gariepinus) from bitumen-polluted River Oluwa, Nigeria. Environ Monit Assess 188, 71 (2016). https://doi.org/10.1007/s10661-015-5003-7
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DOI: https://doi.org/10.1007/s10661-015-5003-7