Dissipation kinetics of emamectin benzoate and lufenuron residues in cabbage grown under field conditions
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Residue analysis of emamectin benzoate and lufenuron in cabbage matrices and soil was developed using a quick, easy, cheap, effective, rugged, and safe (QuEChERS) method and ultra high-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). The samples were extracted with 1 % acetic acid in acetonitrile (v/v) or 1 % acetic acid in acetonitrile/water (5:1, v/v) and cleaned up by dispersive solid-phase extraction. Mean recoveries and relative standard deviations (RSDs) in all samples ranged 87.8–100.0 % and 3.6–12.6 % for emamectin benzoate and 87.8–104.8 % and 6.2–11.5 % for lufenuron, respectively. The validated method was used to evaluate the dissipation rate of emamectin benzoate and lufenuron in cabbage and soil as well as the residual levels in harvested cabbage and soil at different preharvest intervals (PHI). The half-lives of emamectin benzoate and lufenuron were 1.08–2.70 and 1.74–5.04 days in cabbage, and 1.42–4.01 and 0.94–6.18 days in soil, respectively. The terminal residues were below the China maximum residue limits (MRLs) at 3 days for emamectin benzoate (0.1 mg kg−1) and European Union MRLs at 5 days for lufenuron (0.5 mg kg−1), which suggested that 5 days could be recommended as the PHI for the commercial formulation of emamectin benzoate and lufenuron application in the Chinese cabbage field.
KeywordsEmamectin benzoate Lufenuron Dissipation Residues Cabbage
The authors acknowledge the Syngenta (China) Investment Co., Ltd. for providing the necessary financial support to accomplish this project.
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Conflict of interest
The authors declare that they have no conflict of interest.
- Ahire, K. C., Arora, M. S., & Mukherjee, S. N. (2008). Development and application of a method for analysis of lufenuron in wheat flour by gas chromatography–mass spectrometry and confirmation of bio-efficacy against Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae). Journal of Chromatography B, 861(1), 16–21.CrossRefGoogle Scholar
- Ahmed, S., Qureshi, F. A., Islam, A., & Adnan, A. (2011). Emamectin benzoate residue analysis in ground and terrestrial field water in Lahore suburban area. African Journal of Pure & Applied Chemistry, 5(13), 457–462.Google Scholar
- Aiyalu, R., Thangadurai, B., Krishnadas, M., Thomas, S. J., & Sandeep, N. (2013). Analysis of emamectin benzoate residues in cauliflower using HPLC. Research Journal of Pharmacy & Technology, 6(10), 1157–1160.Google Scholar
- Bletsou, A. A., Hanafi, A. H., Dasenaki, M. E., & Thomaidis, N. S. (2013). Development of specific LC-ESI-MS/MS methods to determine bifenthrin, lufenuron, and iprodione residue levels in green beans, peas, and chili peppers under Egyptian field conditions. Food Analytical Methods, 6(4), 1099–1112.CrossRefGoogle Scholar
- Boomathi, N., Kumaran, N., Kumar, B. V., Kuttalam, S., & Gunasekaran, K. (2009). Harvest time residues of emamectin benzoate in cotton. Madras Agricultural Journal, 96, 213–216.Google Scholar
- Cheng, Y., Li, S. X., Zhang, J., Mu, W., & Liu, F. (2012). Optimized the impact factors of fluorescence derivatization reaction and the application in the determination of emamectin benzoate in soil. Journal of Agro Environment Science, 31(12), 2506–2512.Google Scholar
- European Commission (2005). Pesticide EU-MRLs Regulation (EC) No. 396/2005. http://ec.europa.eu/food/plant/pesticides/max_residue_levels/eu_rules_en.htm.
- Hanafi, A., Garau, V. L., Caboni, P., Sarais, G., & Cabras, P. (2010). Minor crops for export: a case study of boscalid, pyraclostrobin, lufenuron and lambda-cyhalothrin residue levels on green beans and spring onions in Egypt. Journal of Environmental Science & Health Part B, 45(6), 493–500.CrossRefGoogle Scholar
- Hayes, J. M., & Farer, L. J. (2005). Comparison study of two procedures for the determination of emamectin benzoate in medicated fish feed. Journal of AOAC International, 88(2), 468–471.Google Scholar
- He, C. X., & Yu, X. C. (2012). An overview of the trend and future development of the world’s major vegetable production. Vegetables, 12, 1–6 (In Chinese).Google Scholar
- Hotz, R. P., Hassler, S., & Maurer, M. P. (2000). Determination of lufenuron in canine skin layers by radioluminography. Schweizer Archiv Fur Tierheilkunde, 142(4), 173–176.Google Scholar
- Janis, J., Mackichan, W., & Hink, F. (2006). High-performance liquid chromatographic determination of CGA-184699 (Lufenuron) in dog and cat blood. Journal of Liquid Chromatography, 16(12), 2595–2604.Google Scholar
- Khay, S., Choi, J. H., Abd El-Aty, A. M., Mamun, M. I., Park, B. J., Goudah, A., Shin, H. C., & Shim, J. H. (2008). Dissipation behavior of lufenuron, benzoylphenylurea insecticide, in/on Chinese cabbage applied by foliar spraying under greenhouse conditions. Bulletin of Environmental Contamination & Toxicology, 81(4), 369–372.CrossRefGoogle Scholar
- Kim-Kang, H., Crouch, L. S., Bova, A., Kono, T., Takemoto, T., Fujita, M., Saka, M., Iwasa, S., Ito, S., & Miyake, S. (2001). Determination of emamectin residues in the tissues of Atlantic salmon (Salmo Salar L.) using HPLC with fluorescence detection. Journal of Agricultural & Food Chemistry, 49(11), 5294–5302.CrossRefGoogle Scholar
- Kondo, M., Yamashita, H., Uchigashima, M., Kono, T., Takemoto, T., Fujita, M., Saka, M., Iwasa, S., Ito, S., & Miyake, S. (2009). Development of an enzyme-linked immunosorbent assay for residue analysis of the insecticide emamectin benzoate in agricultural products. Journal of Agricultural & Food Chemistry, 57, 359–364.CrossRefGoogle Scholar
- Kuo, J., Buday, C., Aggelen, G. V., Ikonomou, M. G., & Pasternak, J. (2010). Acute toxicity of emamectin benzoate and its desmethyl metabolite to Eohaustorius estuarius. Environmental Toxicology & Chemistry, 29(8), 1816–1820.Google Scholar
- Li, M., Liu, X., Dong, F., Xu, J., Kong, Z., Li, Y., & Zheng, Y. (2013b). Simultaneous determination of cyflumetofen and its main metabolite residues in samples of plant and animal origin using multi-walled carbon nanotubes in dispersive solid-phase extraction and ultrahigh performance liquid chromatography-tandem mass spectrometry. Journal of Chromatography A, 1300, 95–103.CrossRefGoogle Scholar
- Ministry of Agriculture, P.R. China and National Health, & Family Planning Commission, P.R. China. (2014). National Food Safety Standard -- Maximum Residue Limits for Pesticides in Food. GB 2763–2014.Google Scholar
- Senguttuvan, K., & Kuttalam, S. (2014). Dissipation dynamics of lufenuron 5.4 EC residues in cauliflower. Trends in Biosciences, 7(15), 1917–1920.Google Scholar
- Tan, H. H., Su, B. L., Zhang, C. L., Zeng, D. Q., & Wang, T. T. (2010). Dynamic analysis of lufehuron residues in/on tomato and bean. Agrochemicals, 49(6), 432–433 (In Chinese).Google Scholar
- The Ministry of Agriculture, P. R. China. (2004). Guideline on pesticide trials. Document No. NY/T788-2004.Google Scholar
- Vijayasree, V., Bai, H., Mathew, T. B., George, T., Xavier, G., Kumar, N. P., & Visalkumar, S. (2014). Dissipation kinetics and effect of different decontamination techniques on the residues of emamectin benzoate and spinosad in cowpea pods. Environmental Monitoring & Assessment, 186(7), 4499–4506.CrossRefGoogle Scholar
- Zhang, Y., Wu, Y., Hu, J., Wang, H., Pan, C., & Liu, F. (2008). Determination of emamectin benzoate residue in vegetables by high performance liquid chromatography with fluorescence detection. Chinese Journal of Chromatography, 26(1), 110–112.Google Scholar