Advertisement

Determination and analysis of the dissipation and residue of cyprodinil and fludioxonil in grape and soil using a modified QuEChERS method

  • Wen Zhang
  • Hongyu Chen
  • Xinwei Han
  • Zaihui Yang
  • Mingming Tang
  • Jing Zhang
  • Song Zeng
  • Deyu Hu
  • Kankan Zhang
Article

Abstract

A simple and accurate method coupled with a gas chromatography-nitrogen phosphorus detector was developed to detect cyprodinil and fludioxonil in grape and soil. The accuracy and precision of the method in detecting the two fungicides were evaluated by conducting intra- and inter-day recovery experiments. The limits of detection were 0.017 mg/kg for cyprodinil and 0.030 mg/kg for fludioxonil. The limits of quantitation were 0.05 mg/kg for cyprodinil and 0.10 mg/kg for fludioxonil in grape and soil. The recoveries of the fungicides in grape and soil were investigated at three spiked levels and were found to range from 85.81 to 102.94 % for cyprodinil and from 92.00 to 106.86 % for fludioxonil, with relative standard deviations below 7 %. Field experiments were conducted in two experimental locations in China. The half-lives of cyprodinil were 9.6–20.8 days in grape and 5.8–15.6 day in soil, and the half-lives of fludioxonil were 6.2–7.2 days in grape and 6.0–12.1 days in soil. When the cyprodinil and fludioxonil 62 % water-dispersible granule formulation was sprayed at a low dosage three times, terminal residues of cyprodinil and fludioxonil were below 1.0 mg/kg in grape 14 days after harvest. This work may serve as a reference to establish the maximum residue limits for cyprodinil and fludioxonil in grape and promote the proper and safe use of these two fungicides.

Keywords

Cyprodinil Fludioxonil Grape Soil Residue Dissipation 

Notes

Acknowledgments

The authors thank the Special Fund for Agro-scientific Research in the Public Interest of China (No. 201203022), the Major Science and Technology Program of Guizhou Province (No. 20136024), the National Natural Science Foundation of China (No. 21402034), and the Science and Technology Program of Guizhou Province (No. 20147664).

References

  1. Al-Rahman, S. H. A., Alnaz, M. M., & Ahmed, N. S. (2012). Dissipation of fungicides, insecticides, and acaricide in tomato using HPLC-DAD and QuEChERS methodology. Food Analytical Methods, 5, 564−570.Google Scholar
  2. Arias, M., Torrente, A. C., Lpez, E., Soto, B., & Simal-Gandara, J. (2005). Adsorption-desorption dynamics of cyprodinil and fludioxonil in vineyard soils. Journal of Agricultural and Food Chemistry, 53, 5675−5681.Google Scholar
  3. Čuš, F., Česnik, H. B., Bolta, Š. V., & Gregorčič, A. (2010). Pesticide residues in grapes and during vinification process. Food Control, 21, 1512−1518.Google Scholar
  4. González, C. F., Otero, R. R., Grande, B. C., & Gándara, J. S. (2003). Determination of fungicide residues in white grapes for winemaking by gas chromatography with mass spectrometric detection and assessment of matrix effects. Journal of AOAC International, 86, 1008−1014.Google Scholar
  5. González-Rodríguez, R. M., Noguerol-Pato, R., González-Barreiro, C., Cancho-Grande, B., & Simal-Gándara, J. (2011). Application of new fungicides under good agricultural practices and their effects on the volatile profile of white wines. Food Research International, 44, 397−403.Google Scholar
  6. Huan, Z. B., Xu, Z., Lv, D. Z., Xie, D. F., & Luo, J. H. (2013). Dissipation and residues of difenoconazole and azoxystrobin in bananas and soil in two agro−climatic zones of China. Bulletin of Environmental Contamination and Toxicology, 91, 734−738.Google Scholar
  7. Lehotay, S. J., Son, K. A., Kwon, H., Koesukwiwat, U., Fu, W. S., Mastovska, K., Hoh, E., & Leepipatpiboon, N. (2010). Comparison of QuEChERS sample preparation methods for the analysis of pesticide residues in fruits and vegetables. Journal of Chromatography A, 1217, 2548−2560.Google Scholar
  8. Marín, A., Oliva, J., Garcia, C., Navarro, S., & Barba, A. (2003). Dissipation rates of cyprodinil and fludioxonil in lettuce and table grape in the field and under cold storage conditions. Journal of Agricultural and Food Chemistry, 51, 4708−4711.Google Scholar
  9. Melo, A., Aguiar, A., Mansilha, C., Pinho, O., & Ferreira, I. M. P. L. V. O. (2012). Optimisation of a solid-phase microextraction/HPLC/diode array method for multiple pesticide screening in lettuce. Food Chemistry, 130, 1090−1097.Google Scholar
  10. Otero, R. R., Ruiz, C. Y., Grande, B. C., & Gándara, J. S. (2002). Solid-phase microextraction-gas chromatographic-mass spectrometric method for the determination of the fungicides cyprodinil and fludioxonil in white wine. Journal of Chromatography A, 942, 41−52.Google Scholar
  11. Payá, P., Anastassiades, M., Mack, D., Sigalova, I., Tasdelen, B., Oliva, J., & Barba, A. (2007). Analysis of pesticide residues using the Quick Easy Cheap Effective Rugged and Safe (QuEChERS) pesticide multiresidue method in combination with gas and liquid chromatography and tandem mass spectrometric detection. Analytical and Bioanalytical Chemistry, 389, 1697−1714.Google Scholar
  12. The statistics report of wine industry in world (2013) International organization of Vine & wine. http://finance.chinanews.com/wine/2013/06-17/4933464.shtml. Accessed June 2013.
  13. Vaquero-Fernández, L., Sáenz-Hernáez, A., Sanz-Asensio, J., Fernández-Zurbano, P., Sainz-Ramírez, M., Pons-Jubera, B., López-Alonso, M., Epifanio-Fernández, S I., & Martínez-Soria, M. T. (2008). Determination of cyprodinil and fludioxonil in the fermentative process of must by high-performance liquid chromatography-diode array detection. Journal of the Science of Food and Agriculture, 88, 1943−1948.Google Scholar
  14. Wilkowska, A., & Biziuk, M. (2011). Determination of pesticide residues in food matrices using the QuEChERS methodology. Food Chemistry, 125, 803−812.Google Scholar

Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • Wen Zhang
    • 1
  • Hongyu Chen
    • 1
  • Xinwei Han
    • 1
  • Zaihui Yang
    • 1
  • Mingming Tang
    • 1
  • Jing Zhang
    • 1
  • Song Zeng
    • 1
  • Deyu Hu
    • 1
  • Kankan Zhang
    • 1
  1. 1.State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of EducationGuizhou UniversityGuiyangPeople’s Republic of China

Personalised recommendations