Comparison of arsenic uptake ability of barnyard grass and rice species for arsenic phytoremediation

  • Razia Sultana
  • Katsuichiro Kobayashi
  • Ki-Hyun Kim


In this research, the relative performance in arsenic (As) remediation was evaluated among some barnyard grass and rice species under hydroponic conditions. To this end, four barnyard grass varieties and two rice species were selected and tested for their remediation potential of arsenic. The plants were grown for 2 weeks in As-rich solutions up to 10 mg As L−1 to measure their tolerance to As and their uptake capabilities. Among the varieties of plants tested in all treatment types, BR-29 rice absorbed the highest amount of As in the root, while Nipponbare translocated the maximum amount of As in the shoot. Himetainubie barnyard grass produced the highest biomass, irrespective of the quantity of As in the solution. In all As-treated solutions, the maximum uptake of As was found in BR-29 followed by Choto shama and Himetainubie. In contrast, while the bioaccumulation factor was found to be the highest in Nipponbare followed by BR-29 and Himetainubie. The results suggest that both Choto shama and Himetainubie barnyard grass varieties should exhibit a great potential for As removal, while BR-29 and Nipponbare rice species are the best option for arsenic phytoremediation.


Arsenic Phytoremediation Barnyard grass Rice Biotypes Bioconcentration factor 



The authors are grateful to Dr. Keiko Yamaji, associate professor, Graduate School of Life and Environmental Sciences, University of Tsukuba, for her valuable suggestions during the entire experiment and during the statistical analysis. The authors thankfully acknowledge the Ministry of Education, Sports, Culture, Science and Technology, Japan, for providing financial support in the form of Monbukagakusho (MEXT) Scholarship for the completion of this research. The third author acknowledges the partial support for his engagement in the data evaluation by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning (grant number 2013004624).


  1. Abedin, M. J., & Meharg, A. A. (2002). Relative toxicity of arsenite and arsenate on germination and early seedling growth of rice (Oryza sativa L.). Plant and Soil, 243, 57–66.CrossRefGoogle Scholar
  2. Ahmed, K. M. (2000). Groundwater arsenic contamination in Bangladesh: an overview. In: Bhattacharya, P., & Welch, A. H. (Eds.). Arsenic in groundwater of sedimentary aquifers. Proceeding of 31st International Geological Congress, Rio de Janerio, Brazil. 3–11.Google Scholar
  3. Alam, M. D., & Sattar, M. A. (2000). Assessment of arsenic contamination in soils and waters in some areas of Bangladesh. Water Science and Technology, 42, 185–192.Google Scholar
  4. Ali, H., Naseer, M., & Sajad, M. A. (2012). Phytoremediation of heavy metals by Trifolium alexandrinum. International Journal of Environmental Science, 2, 1459–1469.Google Scholar
  5. Baker, A. J. M. (1981). Accumulators and excluders—strategies in the response of plants to heavy metals. Journal of Plant Nutrition, 3, 1–4.CrossRefGoogle Scholar
  6. Cox, M. S., Bell, P. F., & Kovar, J. L. (1996). Different tolerance of canola to arsenic when grown hydroponically or in soil. Journal of Plant Nutrition, 19, 1599–1610.CrossRefGoogle Scholar
  7. Banerjee, D. M. (2000). Some comments on the source of arsenic in the Bengal Deltaic sediments. In: Bhattacharya P. And Welch A. H. (Eds.). Arsenic in groundwater of sedimentary aquifers. Proceedings of 31st International geological congress, Rio de Janerio, Brazil. 15–17.Google Scholar
  8. Cai, Y., Georgiadis, M., & Fourqurean, J. W. (2000). Determination of arsenic in sea grass using inductively coupled plasma mass spectrometry. Spectrochimica Acta Part B, 55, 1411–1422.CrossRefGoogle Scholar
  9. Chakraborti, A. K., & Das, D. K. (1997). Arsenic pollution and its environmental significance. Journal of Interacademicia, 1, 262–276.Google Scholar
  10. Cunningham, S. D., & Lee, C. R. (1995). Phytoremediation: plant-based remediation of contaminated soils and sediments. Bioremediation: Science and Applications, (H. D. Skipper and R. F. Turco, Eds.), SSSA Special Publication No 43. SSSA, ASA and CSSA, Madison, WI, 145–156.Google Scholar
  11. Dhor, P. K., et al. (1997). Ground water arsenic calamities in Bangladesh. Current Science, 73, 48–59.Google Scholar
  12. Efroymson, R. A., Sample, B. E., & Suter, G. W. (2001). Uptake of inorganic chemicals from soil by plant leaves: regressions of field data. Environmental Toxicology and Chemistry, 20, 2561–2571.CrossRefGoogle Scholar
  13. Fazal, M. A., Kawachi, T., & Ichio, E. (2001). Validity of the latest research findings on causes of groundwater arsenic contamination in Bangladesh. Water International, 26, 380–389.CrossRefGoogle Scholar
  14. Hopenhayn, C. (2006). Arsenic in drinking water: impact on human health. Elements, 2, 103–107.CrossRefGoogle Scholar
  15. Lombi, E., Zhao, F. J., Dunham, S. J., & Mcgrath, S. P. (2000). Cadmium accumulation in populations of Thlaspi caerulescens and Thlaspi goesingense. New Phytologist, 145, 11–20.CrossRefGoogle Scholar
  16. Lombi, E., Zhao, F. J., Dunham, S. J., & Mcgrath, S. P. (2001). Phytoremediation of heavy metal-contaminated soils: natural hyperaccumulation versus chemically enhanced phytoextraction. Journal of Environmental Quality, 30, 1919–1926.CrossRefGoogle Scholar
  17. Ma, L. Q., Kommar, K. M. M., Tu, C., Zhang, W., Cai, Y., & Kennely, E. (2001). A fern that hyperaccumulates arsenic. Nature, 409, 579.CrossRefGoogle Scholar
  18. Malik, R. N., Husain, S. Z., & Nazir, I. (2010). Heavy metal contamination and accumulation in soil and wild plant species from industrial area of Islamabad, Pakistan. Pakistan Journal of Botany, 42, 291–301.Google Scholar
  19. Marin, A. R., Masscheleyn, P. H., & Patrick, W. H. (1992). The influence of chemical form and concentration of arsenic on rice growth and tissue arsenic concentration. Plant and Soil, 139, 175–183.CrossRefGoogle Scholar
  20. McGrath, S. P., Zhao, F. J., & Lombi, E. (2002). Phytoremediation of metals, metalloids and radionuclides. Advances in Agronomy, 75, 1–56.CrossRefGoogle Scholar
  21. Meharg, A. A., & Rahman, M. M. (2003). Arsenic contamination of Bangladesh paddy field soils: implications for rice contribution to arsenic consumption. Environmental Science and Technology, 37, 229–234.CrossRefGoogle Scholar
  22. Pence, N. S., Larsen, P. B., Ebbs, S. D., Letham, D. L. D., Lasat, M. M., Garvin, D. F., Eide, D., & Kochian, L. V. (2000). The molecular physiology of heavy metal transport in the Zn/Cd hyperaccumulator Thlaspi caerulescens. Proceedings of the National Academy of Sciences USA, 97, 4956–4960.CrossRefGoogle Scholar
  23. Rahman, M. A., Hasegawa, H., Ueda, K., Maki, T., & Rahman, M. M. (2008). Influence of phosphate and iron ions in selective uptake of arsenic species by water fern (Salvinia natans L.). Chemical Engineering Journal, 145, 179–184.CrossRefGoogle Scholar
  24. Raskin, I., Smith, R. D., & Salt, D. E. (1997). Phytoremediation of metals: using plants to remove pollutants from the environment. Current Opinion in Biotechnology, 8, 221–226.CrossRefGoogle Scholar
  25. Robinson, B. H., Green, S., & Mills, T. (2003b). Assessment of phytoremediation as best management practice for degraded environments. In L. D Currie, R. B Stewart, and C. W. N Anderson (Eds.) Environmental Management using Soil–Plant Systems, Occasional Report No. 16. (pp.39-49), Fertilizer and Lime Research Centre, Massey University, Palmerston North, New Zealand.Google Scholar
  26. Schwitzguebel. (2001). Hype or hope: the potential of phytoremediation as an emerging green technology. Remediation: The Journal of Environmental Cleanup, Costs, Technologies and Techniques, 11, 63–77.CrossRefGoogle Scholar
  27. Smith, A. H., Lingas, E. O., & Rahman, M. (2000). Contamination of drinking-water by arsenic in Bangladesh: a public health emergency. Bulletin of World Health Organization, 78, 1093.Google Scholar
  28. Sultana, R., & Kobayashi, K. (2011a). Potentiality of barnyard grass and rice for arsenic contaminated soil. Weed Biology and Management, 11, 12–17.CrossRefGoogle Scholar
  29. Sultana, R., & Kobayashi, K. (2011b). Arsenic uptake by barnyard grass and rice in two types of soils. Bangladesh Journal of Crop Science, 21(1), 159–166.Google Scholar
  30. Sultana, R., & Zaman, M. W. (2008). Phytoremediation potential of some naturally grown weeds for arsenic contaminated soils of Bangladesh. Annals of Bangladesh Agriculture, 12, 99–105.Google Scholar
  31. Sultana, R., Zaman, M. W., Chowdhury, M. A. K., & Islam, S. M. N. (2005). Effect of arsenic on growth and biomass production of arsenic hyperaccumulating weeds. Bangladesh Journal of Crop Science, 16(2), 375–380.Google Scholar
  32. Ullah, S.M. (1998). Arsenic contamination of groundwater and irrigated soils in Bangladesh. Proc. Of the International conference on arsenic pollution of groundwater in Bangladesh: causes, effects and remedies, 8–12 February 1998, Dhaka Community Hospital, Dhaka, Bangladesh. 133.Google Scholar
  33. UNICEF. (2009). Bangladesh National Drinking Water Quality Survey of 2009. Bangladesh Bureau of Statistics.Google Scholar
  34. Visoottiviseth, P., Francesconi, K., & Sridokchan, W. (2002). The potential of Thai indigenous plant species for the phytoremediation of arsenic contaminated land. Environmental Pollution, 118, 453–461.CrossRefGoogle Scholar
  35. Walsh, L. M., & Keeney, D. R. (1975). Behavior and phytotoxicity of inorganic arsenicals in soils. In E.A. Woolson (Ed.), Arsenical pesticides (pp. 35–52). American Chemical Society Symposium series7, American Chemical Society, Washington DC.Google Scholar
  36. Yoshiba, M. (1990). Experimental methods for plant nutrition. In the editorial committee of Experimental Methods for Plant Nutrition (Ed.), Plant cultivation (pp. 3–4). Hakuyusha Ltd. Tokyo (in Japanese).Google Scholar
  37. Yoon, J., Cao, X., Zhou, Q., & Ma, L. Q. (2006). Accumulation of Pb, Cu, and Zn in native plants growing on a contaminated Florida site. Science of the Total Environment, 368, 456–464.CrossRefGoogle Scholar
  38. Zaman, M. W., Mitra, N., & Sultana, R. (2006). Phytoremediation of arsenic contaminated soils with naturally grown weeds. Bangladesh Journal of Agricultural science, 33(1), 73–79.Google Scholar
  39. Zaman, M.W. (2002). Environmental impacts of groundwater abstraction in Barind Area. Component-B: Water quality and agro-ecology. Annual workshop of ARMP contract Research, BARC, 3 April, Dhaka, Bangladesh.Google Scholar
  40. Zaman, M.W., Mollah, M.O.G., Rahman, M.M., & Nizamuddin, M. (2005). Identification of arsenic hyperaccumulating weeds for the remediation of arsenic contaminated soil. Proceedings of the 9th international symposium on soil and plant analysis, Cancun, Mexico, 14.Google Scholar
  41. Zhang, W., Cai, Y., Tu, C., & Ma, L. Q. (2002). Arsenic speciation and distribution in an arsenic hyperaccumulating plants. Science of the Total Environment, 300, 167–177.CrossRefGoogle Scholar
  42. Zhao, F. J., Lombi, E., & McGrath, S. P. (2003). Assessing the potential for zinc and cadmium phytoremediation with the hyperaccumulator Thlaspi caerulescens. Plant and Soil, 249, 37–43.CrossRefGoogle Scholar

Copyright information

© Springer International Publishing Switzerland 2014

Authors and Affiliations

  • Razia Sultana
    • 1
    • 2
  • Katsuichiro Kobayashi
    • 1
  • Ki-Hyun Kim
    • 3
  1. 1.Graduate School of Life and Environmental SciencesUniversity of TsukubaTsukubaJapan
  2. 2.Department of Agricultural ChemistryBangladesh Agricultural UniversityMymensinghBangladesh
  3. 3.Department of Civil and Environmental EngineeringHanyang UniversitySeoulSouth Korea

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