Environmental Monitoring and Assessment

, Volume 185, Issue 10, pp 7979–7991 | Cite as

Speciation of As(III) and As(V) in water and sediment using reverse-phase ion-pair high-performance liquid chromatography-neutron activation analysis (HPLC-NAA)

  • Delali Tulasi
  • Dennis Adotey
  • Andrews Affum
  • Derick Carboo
  • Yaw Serfor-Armah


Total As content and the As species distribution in water and sediments from the Kwabrafo stream, a major water body draining the Obuasi gold mining community in southwestern Ghana, have been investigated. Total As content was determined by instrumental neutron activation analysis (INAA). Ion-pair reverse phase high-performance liquid chromatography-neutron activation analysis (HPLC-NAA) was used for speciation of As species. Solid phase extraction with phosphate buffer was used to extract soluble As species from lyophilized sediment. The mass balance after phosphate extraction of soluble As species in sediment varied from 89 to 96 %. Compositionally appropriate reference material International Atomic Energy Agency (IAEA)-Lake Sediment (SL)-1 was used to check the validity of INAA method for total As determination. The measured values are in good agreement with the IAEA recommended value and also within the 95 % confidence interval. The accuracy of the measurement in terms of relative deviation from the IAEA recommended value was ±0.83 %. “In-house” prepared As(III) and As(V) standards were used to validate the HPLC-INAA method used for the As species determination. Total As concentration in the water samples ranged from 1.15 to 9.20 mg/L. As(III) species in water varied from 0.13 to 0.7 mg/L, while As(V) species varied from 0.79 to 3.85 mg/L. Total As content in sediment ranged from 2,134 to 3,596 mg/kg dry mass. The levels of As(III) and As(V) species in the sediment ranges from 138 to 506 mg/kg dry mass and 156 to 385 mg/kg dry mass, respectively.


Arsenic Speciation Ghana HPLC-NAA Sediment Water 



The authors would like to thankfully acknowledge Messers Ekow Quagraine and Nick Opata, the technologists at the National Nuclear Research Institute of Ghana for their contribution during preparation, irradiations and counting of samples. We also thank Mr. Khalid Ahmed for his assistance during the sampling.


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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Delali Tulasi
    • 1
  • Dennis Adotey
    • 1
    • 3
  • Andrews Affum
    • 3
  • Derick Carboo
    • 2
  • Yaw Serfor-Armah
    • 1
    • 3
  1. 1.School of Nuclear and Allied SciencesUniversity of GhanaLegonGhana
  2. 2.Department of ChemistryUniversity of GhanaLegonGhana
  3. 3.Ghana Atomic Energy CommissionNational Nuclear Research InstituteLegonGhana

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