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Environmental Monitoring and Assessment

, Volume 185, Issue 8, pp 6553–6562 | Cite as

Assessment of oxidative stress markers and concentrations of selected elements in the leaves of Cassia occidentalis growing wild on a coal fly ash basin

  • Amit Love
  • B. D. Banerjee
  • C. R. Babu
Article

Abstract

Assessment of oxidative stress levels and tissue concentrations of elements in plants growing wild on fly ash basins is critical for realistic hazard identification of fly ash disposal areas. Hitherto, levels of oxidative stress markers in plants growing wild on fly ash basins have not been adequately investigated. We report here concentrations of selected metal and metalloid elements and levels of oxidative stress markers in leaves of Cassia occidentalis growing wild on a fly ash basin (Badarpur Thermal Power Station site) and a reference site (Garhi Mandu Van site). Plants growing on the fly ash basin had significantly high foliar concentration of As, Ni, Pb and Se and low foliar concentration of Mn and Fe compared to the plants growing on the reference site. The plants inhabiting the fly ash basin showed signs of oxidative stress and had elevated levels of lipid peroxidation, electrolyte leakage from cells and low levels of chlorophyll a and total carotenoids compared to plants growing at the reference site. The levels of both protein thiols and nonprotein thiols were elevated in plants growing on the fly ash basin compared to plants growing on the reference site. However, no differences were observed in the levels of cysteine, reduced glutathione and oxidized glutathione in plants growing at both the sites. Our study suggests that: (1) fly ash triggers oxidative stress responses in plants growing wild on fly ash basin, and (2) elevated levels of protein thiols and nonprotein thiols may have a role in protecting the plants from environmental stress.

Keywords

Fly ash Cassia occidentalis Metal and metalloid elements Lipid peroxidation Thiols 

Notes

Acknowledgments

A.L. had Junior and Senior Research Fellowship from the Council for Scientific and Industrial Research, India. Financial support of the Ministry of Environment and Forests, Government of India, is acknowledged. We acknowledge the support of Shiv Shankar Prasad Roy in undertaking field work. The views expressed by the corresponding author are his personal and not endorsed by the Ministry of Environment and Forests, Government of India.

References

  1. Adriano, D. C. (2001). Trace elements in terrestrial environments: biogeochemistry, bioavailability and risks of metals (2nd ed.). New York: Springer.CrossRefGoogle Scholar
  2. Baszynski, T., Tukendorf, M., Ruszkowska, M., Skorzynska, E., & Maksymiec, W. (1988). Characteristics of the photosynthetic apparatus of copper non-tolerant spinach exposed to excess copper. Journal of Plant Physiology, 132, 708–713.CrossRefGoogle Scholar
  3. Bidar, G., Garcon, G., Pruvot, C., Dewaele, D., Cazier, F., Douay, F., & Shirali, P. (2007). Behavior of Trifolium repens and Lolium perenne growing in a heavy metal contaminated field: plant metal concentration and phytotoxicity. Environmental Pollution, 147, 546–553.CrossRefGoogle Scholar
  4. Brake, S. S., Jensen, R. R., & Mattox, J. M. (2004). Effects of coal fly ash amended soils on trace element uptake in plants. Environmental Geology, 45, 680–689.CrossRefGoogle Scholar
  5. Carlson, C. L., & Adriano, D. C. (1993). Environmental impacts of coal combustion residues. Journal of Environmental Quality, 22, 227–247.CrossRefGoogle Scholar
  6. Cobbett, C. S. (2000). Phytochelatins and their roles in heavy metal detoxification. Plant Physiology, 123, 825–832.CrossRefGoogle Scholar
  7. Dazy, M., Jung, V., Ferard, J.-F., & Masfaraud, J.-F. (2008). Ecological recovery of vegetation on a coke-factory soil: role of plant antioxidant enzymes and possible implications in site restoration. Chemosphere, 74, 57–63.CrossRefGoogle Scholar
  8. Devi, S. R., & Prasad, M. N. V. (1998). Copper toxicity in Ceratophyllum demersum L. (Coontail), a free floating macrophyte: response of antioxidant enzymes and antioxidants. Plant Science, 138, 157–165.CrossRefGoogle Scholar
  9. Dellantonio, A., Fitz, W. J., Custovic, H., Repmann, F., Schneider, B. U., Grunewald, H., Guber, V., Zgorelec, Z., Zerem, N., Carter, C., Markovic, M., Puschenreiter, M., & Wenzel, W. (2008). Environmental risks of farmed and barren alkaline coal ash landfills in Tuzla, Bosnia and Herzegovina. Environmental Pollution, 153, 677–686.CrossRefGoogle Scholar
  10. Dominguez-Solis, J. R., Lopez-Martin, M. C., Ager, F. J., Ynsa, M. D., Romero, L. C., & Gotor, C. (2004). Increased cysteine availability is essential for cadmium tolerance and accumulation in Arabidopsis thaliana. Plant Biotechnology Journal, 2, 469–476.CrossRefGoogle Scholar
  11. Djurdjevic, L., Mitrovic, M., Pavlovic, P., Gajic, G., & Kostic, O. (2006). Phenolic acids as bioindicators of fly ash deposit revegetation. Archives of Environmental Contamination and Toxicology, 50, 488–495.CrossRefGoogle Scholar
  12. Dwivedi, S., et al. (2007). Growth performance and biochemical responses of three rice (Oryza sativa L.) cultivars grown in fly-ash amended soil. Chemosphere, 67, 140–151.CrossRefGoogle Scholar
  13. Gaitonde, M. K. (1967). A spectrophotometric method for the direct determination of cysteine in the presence of other naturally occurring amino acids. Biochemical Journal, 104, 627–633.Google Scholar
  14. Gillman, G. P., & Sumpter, E. A. (1986). Modification to compulsive exchange method for measuring characteristics of soils. Australian Journal of Soil Research, 24, 61–66.CrossRefGoogle Scholar
  15. Gonnelli, C., Galardi, F., & Gabbrielli, R. (2001). Nickel and copper tolerance and toxicity in three Tuscan populations of Silene paradoxa. Physiologia Plantarum, 113, 507–514.CrossRefGoogle Scholar
  16. Gupta, D. K., Rai, U. N., Tripathi, R. D., & Inouhe, M. (2002). Impacts of fly ash on soil and plant responses. Journal of Plant Research, 115, 401–409.CrossRefGoogle Scholar
  17. Gupta, D. K., et al. (2007). Growth and biochemical parameters of Cicer arietinum L. grown on amended fly ash. Environmental Monitoring and Assessment, 134, 479–487.CrossRefGoogle Scholar
  18. Hartley-Whitaker, J., Ainsworth, G., & Meharg, A. A. (2001). Copper- and arsenate-induced oxidative stress in Holcus lanatus L. clones with differential sensitivity. Plant Cell & Environment, 24, 713–722.CrossRefGoogle Scholar
  19. Haynes, R. J. (2009). Reclamation and revegetation of fly ash disposal sites—challenges and research needs. Journal of Environmental Management, 90, 43–53.CrossRefGoogle Scholar
  20. Heath, R. L., & Packer, L. (1968). Photoperoxidation in isolated chloroplast I. Kinetic and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics, 125, 189–198.CrossRefGoogle Scholar
  21. India Meteorological Department (2008). Mean maximum/minimum temperatures and monthly rainfall over Delhi (Palam), http://121.241.116.157/climatology/plm.htm. Accessed on 15 August 2008
  22. Jackson, M. L. (1973). Soil chemical analysis. New Delhi: Prentice Hall of India Ltd.Google Scholar
  23. Jankowski, J., Ward, C. R., French, D., & Groves, S. (2006). Mobility of trace elements from selected Australian fly ashes and its potential impact on aquatic ecosystems. Fuel, 85, 243–256.CrossRefGoogle Scholar
  24. Kumar, A., et al. (2002). Biochemical responses of Cassia siamea Lamk. grown on coal combustion residue (fly-ash). Bulletin of Environmental Contamination and Toxicology, 68, 675–683.CrossRefGoogle Scholar
  25. Kuzmick, D. M., Mitchelmore, C. L., Hopkins, W. A., & Rowe, C. L. (2007). Effects of coal combustion residues on survival, antioxidant potential, and genotoxicity resulting from full-lifecycle exposure of grass shrimp (Palaemonetes pugio Holthius). Science of the Total Environment, 373, 420–430.CrossRefGoogle Scholar
  26. Lamothe, P. J., Fries, T. L., & Consul, J. J. (1986). Evaluation of a microwave oven system for the dissolution of geologic samples. Analytical Chemistry, 58, 1881–1886.CrossRefGoogle Scholar
  27. Love, A., Tandon, R., Banerjee, B. D., & Babu, C. R. (2009). Comparative study on elemental composition and DNA damage in leaves of a weedy plant species, Cassia occidentalis, growing wild on weathered fly ash and soil. Ecotoxicology, 18, 791–801.CrossRefGoogle Scholar
  28. Madejon, P., Maranon, T., Murillo, J. M., & Robinson, B. (2004). White poplar (Populus alba) as a biomonitor of trace elements in contaminated riparian forests. Environmental Pollution, 132, 145–155.CrossRefGoogle Scholar
  29. Maleva, M. G., Nekrasova, G. F., Malec, P., Prasad, M. N. V., & Strzalka, K. (2009). Ecophysiological tolerance of Elodea canadensis to nickel exposure. Chemosphere, 77, 392–398.CrossRefGoogle Scholar
  30. Marquez-Garcia, B., Horemans, N., Cuypers, A., Guisez, Y., & Cordoba, F. (2011). Antioxidants in Erica andevalensis: a comparative study between wild plants and cadmium-exposed plants under controlled conditions. Plant Physiology and Biochemistry, 49, 110–115.CrossRefGoogle Scholar
  31. Meers, E., Ruttens, A., Geebelen, W., Vangronsveld, J., Samson, R., Vanbroekhoven, K., Vandegehuchte, M., Diels, L., & Tack, F. M. G. (2005). Potential use of the plant antioxidant network for environmental exposure assessment of heavy metals in soils. Environmental Monitoring and Assessment, 120, 243–267.CrossRefGoogle Scholar
  32. Mishra, L. C., & Shukla, K. N. (1986). Effects of fly-ash deposition on growth, metabolic and dry matter production of maize and soyabean. Environmental Pollution, 42, 1–13.Google Scholar
  33. MoEF (2005). Annual report. Ministry of Environment and Forests, Government of IndiaGoogle Scholar
  34. Murphy, A., & Taiz, L. (1995). Comparison of mettlothionein gene expression and nonprotein thiols in ten Arabidopsis ecotypes: correlation with copper tolerance. Plant Physiology, 109, 945–954.CrossRefGoogle Scholar
  35. Kabata-Pendias, A., & Pendias, H. (2001). Trace elements in soils and plants (3rd ed.). Boca Raton: CRC Press.Google Scholar
  36. Petaloti, C., Triantafyllou, A., Kouimtzis, T., & Samara, C. (2006). Trace elements in atmospheric particulate matter over a coal burning production area of western Macedonia, Greece. Chemosphere, 65, 2233–2243.CrossRefGoogle Scholar
  37. Pichtel, J., & Hayes, J. M. (1990). Influence of fly ash on soil microbial activity and populations. Journal of Environmental Quality, 19, 593–597.CrossRefGoogle Scholar
  38. Polle, A., Schwanz, P., & Rudolf, C. (2001). Developmental and seasonal changes of stress responsiveness in beech leaves (Fagus sylvatica L.). Plant Cell & Environment, 24, 812–829.Google Scholar
  39. Qian, M., Li, X., & Shen, Z. (2005). Adaptive copper tolerance in Elsholzia haichowensis involves production of Cu-induced thiol peptides. Plant Growth Regulation, 47, 65–73.CrossRefGoogle Scholar
  40. Reash, R. J., Lohner, T. W., & Wood, K. V. (2006). Selenium and other trace metals in fish inhabiting a fly ash stream: implications for regulatory tissue thresholds. Environmental Pollution, 142, 397–408.CrossRefGoogle Scholar
  41. Rees, D. G. (1985). Essential statistics. London: Chapman and Hall.Google Scholar
  42. Schutzendubel, A., & Polle, A. (2002). Plant responses to abiotic stresses: heavy metal-induced oxidative stress and protection by mycorrhization. Journal of Experimental Botany, 53, 1351–1365.CrossRefGoogle Scholar
  43. Seth, C. S., Chaturvedi, P. K., & Misra, V. (2008). The role of phytochelatins and antioxidants in tolerance to Cd accumulation in Brassica juncea L. Ecotoxicology and Environmental Safety, 71, 76–85.CrossRefGoogle Scholar
  44. Sinha, S., Rai, U. N., Bhatt, K., Pandey, K., & Gupta, A. K. (2005). Fly-ash-induced oxidative stress and tolerance in Prosopis juliflora L. grown on different amended substrates. Environmental Monitoring and Assessment, 102, 447–457.CrossRefGoogle Scholar
  45. Smirnoff, N. (1998). Plant resistance to environmental stress. Current Opinions in Biotechnology, 9, 214–219.CrossRefGoogle Scholar
  46. Soco, E., & Kalembkiewicz, J. (2007). Investigations of sequential leaching behaviour of Cu and Zn from coal fly ash and their mobility in environmental conditions. Journal of Hazardous Materials, 145, 482–487.CrossRefGoogle Scholar
  47. Tausz, M., Sircelj, H., & Grill, D. (2004). The glutathione system as a stress marker in plant ecophysiology: is a stress-response concept valid? Journal of Experimental Botany, 55, 1955–1962.CrossRefGoogle Scholar
  48. Zhang, J., & Kirkham, M. B. (1996). Antioxidant responses to drought in sunflower and sorghum seedlings. New Phytologist, 132, 361–373.CrossRefGoogle Scholar
  49. Zhang, W., Cai, Y., Downum, K. R., & Ma, L. Q. (2004). Thiol synthesis and arsenic hyperaccumulation in Pteris vittata (Chinese brake fern). Environmental Pollution, 131, 337–345.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  1. 1.Centre for Environmental Management of Degraded Ecosystems (CEMDE), School of Environmental StudiesUniversity of DelhiDelhiIndia
  2. 2.Environmental Biochemistry Laboratory, Department of Biochemistry, University College of Medical SciencesUniversity of DelhiDelhiIndia
  3. 3.Ministry of Environment and ForestsGovernment of IndiaNew DelhiIndia

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