Environmental Monitoring and Assessment

, Volume 185, Issue 3, pp 2755–2765 | Cite as

Indirect prediction of surface ozone concentration by plant growth responses in East Asia using mini-open top chambers

  • Yoshihisa Kohno
  • Hideyuki Matsumura
  • Makoto Miwa
  • Tetsushi Yonekura
  • Keiji Aihara
  • Chanin Umponstira
  • Vo Thanh Le
  • Nguyen Thuy Ngoc
  • Phanm Hung Viet
  • Ma Wei


We developed small and mobile open top chambers (mini-OTC) measuring 0.6 m (W) × 0.6 m (D) × 1.2 m (H) with an air duct of 0.6 m (W) × 0.23 m (D) × 1.2 m (H). The air duct can be filled with activated charcoal to blow charcoal filtered air (CF) into the chamber, as opposed to non-filtered ambient air (NF). Ozone sensitive radish Raphanus sativus cv. Red Chime and rosette pakchoi Brassica campestris var. rosularis cv. ATU171 were exposed to NF and CF in mini-OTCs at different locations in East Asia. A total of 29 exposure experiments were conducted at nine locations, Shanghai, China, Ha Noi, Vietnam, Lampang, Phitsanulok and Pathumtani, Thailand, and Hiratsuka, Kisai, Abiko and Akagi, Japan. Although no significant relationships between the mean concentrations of ambient O3 during the experimental period and the growth responses were observed for either species, multiple linear regression analysis suggested a good relationship between the biomass responses in each species and the O3 concentration, temperature, and relative humidity. The cumulative daily mean O3 (ppb/day) could be indirectly predicted by NF/CF based on the dry weight ratio of biomass, mean air temperature, and relative air humidity.


Indicator plant Dose–response relationship Multiple linear regression analysis East Asia 



Akaike information criteria


Cumulative daily mean ozone (ppb/day)


Charcoal filtered air


Dry weight


Non-filtered air




Open-top chamber


Parts per billion (nL/L)


Relative humidity (%)


Temperature in Celsius


Vapor pressure deficit (kPa)



This research was conducted with financial support from the Global Environmental Research Fund (C-7), Ministry of the Environment, Japan. We greatly appreciate the collaboration and arrangements with Dr. Tran Thi Ngoc Lan, University of Natural Sciences, Ho Chih Minh City, Vietnam, and Dr. Yasuaki Maeda, JICA Expert, Ministry of the Natural Resources and Environment, Ha Noi, Vietnam. We also appreciate Mr. Ideta, Techno Systems Co. Ltd., Tokyo, Japan for his chemical analysis of air quality samples. Additionally, we thank the students and staff of the universities and CERES Inc. at Akagi Testing Center, CRIEPI for their support with the experiments. Finally, we thank Dr. M. Frei, University of Bonn, for his critical review and editorial suggestions regarding this manuscript.


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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Yoshihisa Kohno
    • 1
  • Hideyuki Matsumura
    • 1
  • Makoto Miwa
    • 2
  • Tetsushi Yonekura
    • 2
  • Keiji Aihara
    • 3
  • Chanin Umponstira
    • 4
  • Vo Thanh Le
    • 5
  • Nguyen Thuy Ngoc
    • 5
  • Phanm Hung Viet
    • 5
  • Ma Wei
    • 6
  1. 1.Central Research Institute of Electric Power Industry (CRIEPI)AbikoJapan
  2. 2.Center for Environmental Science in Saitama (CESS)KazoJapan
  3. 3.Kanagawa Environmental Research Center (KERC)HiratsukaJapan
  4. 4.Department of Natural Resources and EnvironmentNaresuan University (NU)PhitsanulokThailand
  5. 5.Center for Environment and Technology for Sustainable Development (CETASD)Ha Noi University of ScienceHa NoiVietnam
  6. 6.School of Life Science and TechnologyShanghai Jiao Tong University (SJTU)ShanghaiPeople’s Republic of China

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