Environmental Monitoring and Assessment

, Volume 185, Issue 1, pp 573–580 | Cite as

Fast simultaneous determination of traces of Cu(II) and Co(II) in soils and sediments with the luminol/perborate chemiluminescent system

  • José A. Murillo Pulgarín
  • Luisa F. García Bermejo
  • Armando Carrasquero Durán


A flow injection analysis method based on ion chromatography and luminol chemiluminescence detection was used for the simultaneous determination of copper (II) and cobalt (II) trace levels in soils and sediments following microwave-assisted acid digestion. Detection was based on chemiluminescence (CL) of the luminol–perborate system in an alkaline medium, which is catalyzed by both transition metals. The concentration and pH of the eluent (oxalic acid) was found to affect CL intensities and retention times, both of which were inversely proportional to the oxalic acid concentration. The calibration curves for both metal ions were linear and allowed a limit of detection of 0.003 μg l−1 for cobalt (II) and 0.014 μg l−1 for cooper (II) to be calculated. The proposed method was successfully used to determine both metal ions in certified reference materials of stream and river sediments and soil samples. Based on the results, the determination is free of interferences from the usual concomitant ions.


Cobalt Copper Chemiluminescence Ion chromatography Microwave digestion Environmental samples 



The authors gratefully acknowledge financial support from the “Consejería de Educación y Ciencia, Junta de Comunidades de Castilla-La Mancha” (project no. PCI-08-0120).


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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • José A. Murillo Pulgarín
    • 1
  • Luisa F. García Bermejo
    • 1
  • Armando Carrasquero Durán
    • 2
  1. 1.Department of Analytical Chemistry and Food TechnologyUniversity of Castilla–La ManchaCiudad RealSpain
  2. 2.Department of ChemistryUniversidad Pedagógica Experimental LibertadorMaracayVenezuela

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