Abstract
Although high concentrations of trace organic pollutants were recorded along the Egyptian Mediterranean Coast and its corresponding coastal wetlands, no published data are available for the levels of phenolic compounds. Thus, this work aimed to investigate the levels of phenolic compounds in sediments of a heavily polluted coastal wetland (Lake Maryut, Egypt). For that purpose, a method was optimized for the extraction and detection of chlorophenols, methylphenols, and nitrophenols in sediments using GC-MS. Sediments were extracted with 0.1 M NaOH/methanol by sonication. Cleanup of sediment extracts using liquid–liquid extraction and SPE was found important to remove most of the interfering co-extracts. The proposed analytical methodology was validated by analysis of matrix spikes. Detection limits were 0.063–0.694 μg/kg dw for sediments. Good recoveries (70–110%) and precision values (RSD < 20%) were obtained from the fortification experiments at the parts per billion level in sediments. The method was applied to investigate the level of contamination with phenols in 19 sediment samples from Lake Maryut. Results revealed that higher concentrations were observed in the main basin (MB) of Lake Maryut affected by the discharge of effluents from a primary wastewater treatment plant, direct discharge of industrial effluents, domestic wastes, and agricultural effluents from Qalaa Drain (QD). Chlorophenols (CPs) were the major group detected in the lake sediments followed by methylphenols (MPs) and nitrophenols (NPs). CPs were dominated by 2-, 4-, and 3-chlorophenols. Concentrations of CPs were higher at the north and northwestern parts of the MB indicating the influence of industrial effluents discharged into the lake. On the other hand, higher concentrations of NPs were observed at the south and southwestern parts of the MB, which is subjected to the discharge of agricultural and domestic effluents via QD. Results of the risk assessment revealed that phenol, cresols, 2,4-dinitrophenol, 4-NP, 2-CP, 2,3,4,6-tetrachlorophenol and 2,4-dimethylphenol are contaminants of concern and that adverse ecological effects could possibly occur to benthic species from the exposure to these pollutants in Lake Maryut and thus phenols should be included in monitoring and pollution prevention programs in the Egyptian aquatic environment affected by anthropogenic activities.
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Abbass, M. M., Abd-Allah, A. M., El-Gindy, K., Ali, H., Tantawy, G., & El-Sebae, A. E. (1992). Residue levels of hexachlorobenzene HCB and polychlorinated hydrocarbons in northern off-Delta Mediterranean Sea water and bottom sediment. Bulletin of the National Institute of Oceanography and Fisheries, 17, 71–77.
Abbassy, M. S. (2000). Pesticides and polychlorinated biphenyls drained into north coast of the Mediterranean Sea, Egypt. Bulletin of Environmental Contamination and Toxicology, 64, 508–517.
Abdel-Moati, M. A. R., & El-Sammak, A. A. (1997). Man-made impacts on the geochemistry of Nile Delta Lakes. A study of metal concentrations in sediments. Water Air and Soil Pollution, 97, 413–429.
Abd-Allah, A. M. (1992). Determination of DDTs and PCBs residues in Abu-Quir and El-Max Bays, Alexandria, Egypt. Toxicology and Environmental Chemistry, 36, 89–97.
Abd-Allah, A. M., & Abbas, M. M. (1994). Residue levels of organochlorine pollutants in the Alexandria Region, Egypt. Toxicology and Environmental Chemistry, 41, 239–247.
Abd-Allah, A. M., & Ali, H. A. (1994). Residue levels of chlorinated hydrocarbons compounds in fish from El-Max Bay and Maryut Lake, Alexandria, Egypt. Toxicology and Environmental Chemistry, 42, 107–114.
Abd-Allah, M. A., & EL-Sebae, A. K. (1995). Hydrocarbon contamination of the Egyptian Mediterranean Coast. Toxicology and Environmental Chemistry, 47, 15–22.
Abd-Allah, A. M., Abbas, M., El-Gindy, K., Ali, H., Tantawy, G., & El Sebae, A. H. (1992). Residue level of organochlorine pesticides in sediments from northern off Nile Delta Mediterranean Sea coast. Toxicology and Environmental Chemistry, 37, 43–47.
Abdel-Mallek, A. Y., Abdel-Kader, M. I. A., & Omar, S. A. (1994). Effect of the herbicide fluazifop-butyl on fungal populations and activity in soil. Water Air Soil Pollut., 86, 151–157.
Abo-Elamayem, M., Saad, M. A., & EL-Sebae, A. H. (1979). Water pollution with organochlorine pesticides in Egyptian Lakes. Proceedings of the International Egyptian-German Seminar on Environmental Protection from Hazards of Pesticides, Alexandria, Egypt, 94–108.
Abu Elamayem, M. M., EL-Sebae, A. H., & Sharaf, I. F. (1982). Occurrence and distribution of chemical pollutants in Lake Maryut, Egypt. Water Air and Soil Pollution, 17, 245–252.
Alonso, M. C., Puig, D., Silgoner, I., Grasserbauer, M., & Barcelo, D. (1998). Determination of priority phenolic compounds in soil samples by various extraction methods followed by liquid chromatography-atmospheric pressure chemical ionization mass spectrometry. Journal of Chromatography A, 823, 231–239.
Bagheri, H., & Saraji, M. (2001). New polymeric sorbent for the solid-phase extraction of chlorophenols from water samples followed by gas chromatography-electron-capture detection. Journal of Chromatography A, 910, 87–93.
Barakat, A. O. (2001). PAHs and petroleum markers in the atmospheric environment of Alexandria City, Egypt. Water Air and Soil Pollution, 189, 289–310.
Barakat, A. O. (2004). Assessment of persistent toxic substances in the environment of Egypt. Environment International, 30, 309–322.
Barakat, A. O., Moonkoo, K., Yoarong, Q., & Wade, T. L. (2002). Organochlorine pesticides and PCB residues in sediments of Alexandria Harbour, Egypt. Marine Pollution Bulletin, 44, 1421–1434.
Barakat, A. O., Mostafa, A. R., Wade, T. L., Sweet, T., & Badr, N. (2010). Spatial distribution and temporal trends of polycyclic aromatic hydrocarbons in sediments from Lake Maryut, Alexandria, Egypt. Water Air and Soil Pollution, 218, 63–80.
Bartak, P., & Cap, L. (1997). Determination of phenols by solid phase microextraction. Journal of Chromatography A, 767, 171–175.
Bartels, P., Ebeling, E., Kramer, B., Kruse, H., Osius, N., Vowinkel, K., et al. (1999). Determination of chlorophenols in urine of children and suggestion of reference values. Fresenius Journal of Analytical Chemistry, 365, 458–464.
Crespin, M. A., Gallego, M., & Valcarcel, M. (2002). Solid-phase extraction method for the determination of free and conjugated phenol compounds in human urine. Journal of Chromatography B, 773, 89–96.
Czaplicka, M. (2001). Determination of phenols and chlorophenols in bottom sediments. Chromatographia Supplement, 53, S470–S473.
Czaplicka, M. (2004). Sources and transformations of chlorophenols in the natural environment. Science of the Total Environment, 322, 21–39.
Dmitruk, U., Piascik, M., Taboryska, B., & Dojlido, L. (2008). Persistent organic pollutants (POPs) in bottom sediments of Vistula River, Poland. Clean- Soil Air Water, 36, 222–229.
EL-Rayis, O. A. (2005). Impact of man's activities on a closed fishing-lake, Lake Maryout in Egypt, as a case study. Mitigation and Adaptation Strategies for Global Change, 10, 145–157.
Gao, J., Liu, L., Liu, X., Zhou, H., Huang, S., & Wang, Z. (2008). Levels and spatial distribution of chlorophenols 2,4-dichlorophenol, 2,4,6-trichlorophenol and pentachlorophenol in surface water of China. Chemosphere, 71, 1181–1187.
Jauregui, O., Moyano, E., & Galceran, M. T. (1997). Liquid chromatography-atmospheric pressure ionization mass spectrometry for the determination o f chloro- and nitrophenolic compounds in tap water and sea water. Journal of Chromatography A, 787, 79–89.
Johnson, L. D., Midgett, M. R., James, R. H., Thomason, M. M., & Manier, M. L. (1989). Screening approach for principal organic hazardous constituents and products of incomplete combustion. Journal of Air Pollution Control Association, 39, 709–713.
Juteau, P., Beaudet, R., McSween, G., Lepine, F., Milot, S., & Bisaillon, J. G. (1995). Anaerobic biodegradation of pentachlorophenol by a methanogenic consortium. Applied Microbiology and Biotechnology, 44, 218–224.
Khairy, M. A., Kolb, M., Mostafa, A. R., EL-Fiky, A., & Bahadir, M. (2009). Risk assessment of polycyclic aromatic hydrocarbons in a Mediterranean semi-enclosed basin affected by human activities. Journal of Hazardous Materials, 170, 389–397.
Kishk, F. M., El-Essawi, T., Abdel-Ghafar, S., & Abou-Donia, M. B. (1976). Hydrolysis of methyl-parathion in soils. Journal of Agriculture and Food Chemistry, 24, 305–307.
Kovacs, A., Kende, A., Mortl, M., Volk, G., Rikker, T., & Torkos, K. (2008). Determination of phenols and chlorophenols as trimethylsilyl derivatives using gas chromatography–mass spectrometry. Journal of Chromatography A, 1194, 139–142.
Leuenberger, Ch, Czuczwa, J., Tremp, J., & Giger, W. (1988). Nitrated phenols in rain: atmospheric occurrence of phytotoxic pollutants. Chemosphere, 17, 511–515.
Long, G. L., & Winefordner, J. D. (1983). Limit of detection—a closer look at the IUPAC definition. Analytical Chemistry, 55, 712A–724A.
Lyytikainen, M., Sormunen, A., Peraniemi, S., & Kukkonen, J. V. K. (2001). Environmental fate and bioavailability of wood preservatives in freshwater sediments near and old sawmill site. Chemosphere, 44, 341–350.
Martins, M. A., Ferreira, I. C., Santos, I. M., Queiroz, M. J., & Lima, N. (2001). Biodegradation of bioaccessible textile azo dyes by Phanerochaete Chrysosporium. Journal of Biotechnology, 89, 91–98.
Masoud, S. (2005). An approach for protection of Alexandria coastal zone from industrial pollution. Emergency plans for the protection of Egyptian Coasts from marine pollution, EEAA, Egypt. http://www.eeaa.gov.eg/reports.
Michałowicz, J., & Duda, W. (2007). Phenols—sources and toxicity. Polish Journal of Environmental Studies, 16, 347–362.
Moder, M., Schrader, S., Franck, U., & Popp, P. (1997). Determination of phenolic compounds in waste water by solid-phase micro extraction. Fresenius Journal of Analytical Chemistry, 357, 326–332.
Mostafa, A. R., Baraka, A. O., Qian, Y., & Wade, T. L. (2003). Composition, distribution and sources of polycyclic aromatic hydrocarbons in sediments of the Western Harbour of Alexandria, Egypt. Journal of Soils and Sediments, 3, 173–179.
Mostafa, A. R., Hegazi, A. H., EL-Gayar, M. Sh., & Andersson, J. T. (2009). Source characterization and the environmental impact of urban street dusts from Egypt based on hydrocarbon distributions. Fuel, 88, 95–104.
Mussmann, P., Levsen, K., & Radeek, W. (1994). Gas chromatographic determination of 483 phenols in aqueous samples after solid phase extraction. Fresenius Journal of Analytical Chemistry, 348, 654–659.
Oberg, T. (2004). Halogenated aromatics from steel production: results of pilot-scale investigation. Chemosphere, 56, 441–448.
Ohlenbusch, G., Kumke, M. U., & Frimmel, F. H. (2000). Sorption of phenols to dissolved organic matter investigated by solid phase microextraction. Science of the Total Environment, 253, 63–74.
Prenner, M. M., Ibrahim, H., Lewis, J. W., & Crane, M. (2006). Toxicity and trace metal concentrations of sediments from Lake Maryut, Alexandria, Egypt. Bulletin of Environmental Contamination and Toxicology, 77, 616–623.
Saad, M. A. H., Ezzat, A. A., EL-Rayis, O. A., & Hafez, H. (1981). Occurrence and distribution of chemical pollutants in Lake Maryut, Egypt. II: heavy metals. Water Air and Soil Pollution, 16, 401–407.
Santana, C. M., Ferrera, Z. S., Padron, M. E. T., & Rodrigues, J. J. S. (2009). Methodologies for the extraction of phenolic compounds from environmental samples: new approaches. Molecules, 14, 298–320.
Takasu, T., Iles, A., & Kiyoshi, H. (2002). Determination of alkylphenols and alkylphenol polyethoxylates by reversed-phase high-performance liquid chromatography and solid-phase extraction. Analytical and Bioanalytical Chemistry, 372, 554–564.
Tang, Z. W., Yang, Z. F., Shen, Z. Y., & Niu, J. F. (2007). Pentachlorophenol residues in suspended particulate matter and sediments from the Yangtze River Catchment of Wuhan, China. Bulletin of Environmental Contamination and Toxicology, 78, 158–162.
Turnes, M. I., Rodrıguez, I., Mejuto, M. C., & Cela, R. (1994). Determination of chlorophenols in drinking water samples at the subnanogram per millilitre level by gas chromatography with atomic emission detection. Journal of Chromatography A, 683, 21–29.
United States Environmental Protection Agency. (2003). Ecological screening levels of region V for phenolic compounds in sediments. (http://www.epa.gov/reg5rcra/ca/edql.htm).
van Leeuwen, J. A., Nicholson, B. C., Hayes, K. P., & Mulcahy, D. E. (1996). Persistence of chlorophenolic compounds from pulp and paper mill discharges in Lake Bonney, South-eastern South Australia. Marine Freshwater Research, 1996(47), 929–936.
Vidal, J. L. M., Vega, A. B., Frenich, A. G., Gonzalez, F. J. E., & Liebanas, F. J. A. (2004). Determination of fifteen priority phenolic compounds in environmental samples from Andalusia (Spain) by liquid chromatography-mass spectrometry. Analytical and Bioanalytical Chemistry, 379, 125–130.
Wang, Y. J., Ho, Y. S., Jeng, J. H., Su, H. J., & Lee, C. C. (2000). Different cell death mechanisms and gene expression in human cells induced by pentachlorophenol and its major metabolite tetrachlorohydroquinone. Chemistry–Biology Interaction, 128, 173–188.
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Khairy, M.A. Assessment of priority phenolic compounds in sediments from an extremely polluted coastal wetland (Lake Maryut, Egypt). Environ Monit Assess 185, 441–455 (2013). https://doi.org/10.1007/s10661-012-2566-4
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DOI: https://doi.org/10.1007/s10661-012-2566-4