Environmental Monitoring and Assessment

, Volume 166, Issue 1–4, pp 573–580 | Cite as

Characterization of dissolved organic carbon at low levels in environmental waters by microfluidic-chip-based capillary gel electrophoresis with a laser-induced fluorescence detector

  • Shuanglong Shen
  • Yan Li
  • Shin-ichi Wakida


A microfluidic analytical system for characterization of dissolved organic carbon (DOC) in environmental waters, based on a capillary gel electrophoresis (CGE) device with a laser-induced fluorescence (LEF) detector, was developed. The applied voltage and the running buffer were investigated to control the simple floating injection and CGE separation for convenient cross-type microchips made from polymethylmethacylate. We obtained reproducible peaks for standard organic solutions and the determination time is less than 70 s. The values of the relative standard deviation (RSD) were 0.17–2.01% for repetitive injection (n = 12). We demonstrated high-throughput characterization of DOC in environmental water from the Biwa Lake and the Hino River using microfluidic chip and determined that the content of DOC in the Biwa Lake changed with the seasons.


Characterization Microfluidic chip Capillary gel electrophoresis (CGE) Laser-induced fluorescence (LEF) Dissolved organic carbon (DOC) 


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  1. Alberts, J. J., Takács, M., & Egeberg, P. K. (2002). Total luminescence spectral characteristics of natural organic matter (NOM) size fractions as defined by ultrafiltration and high performance size exclusion chromatography (HPSEC). Organic Geochemistry, 33, 817–828.CrossRefGoogle Scholar
  2. Allpike, B. P., Heitz, A., Joll, C. A., et al. (2005). Size exclusion chromatography to characterize DOC removal in drinking water treatment. Environmental Science & Technology, 39, 2334–2342.CrossRefGoogle Scholar
  3. Bikas, V., Steve, W. W., Shelley, J. C., et al. (1997). Reduction of chloride ion in chemical oxygen demand determinations using bismuth-based adsorbents. Analytica Chimica Acta, 357, 167–175.CrossRefGoogle Scholar
  4. Coble, P. G. (1996). Characterization of marine and terrestrial DOM in seawater using excitation-emission matrix spectroscopy. Marine Chemistry, 51, 325–346.CrossRefGoogle Scholar
  5. Drewes, J. E., Reinhard, M., & Fox, P. (2003). Comparing microfiltration-reverse osmosis and soil-aquifer treatment for indirect potable reuse of water. Water Research, 37, 3612–3621.CrossRefGoogle Scholar
  6. Dunkelog, R., Ruttinger, H. H., & Peisker, K. (1997). Comparative study for the separation of aquatic humic substances by electrophoresis. Journal of Chromatography A, 777, 355–362.CrossRefGoogle Scholar
  7. Elliott, S., Lead, J. R., & Baker, A. (2006). Thermal quenching of fluorescence of freshwater, planktonic bacteria. Analytica Chimica Acta, 564, 219–225.CrossRefGoogle Scholar
  8. Jardim, W. F., & Rohweder, J. (1989). Chemical oxygen demand (COD) using microwave digestion. Journal of Water Resources, 23, 1069–1071.Google Scholar
  9. Hautala, K., Peuravuori, J., & Pihlaja, K. (2000). Measurement of aquatic humus content by spectroscopic analysis. Water Research, 34, 246–258.CrossRefGoogle Scholar
  10. Huber, S. A., & Frimmel, F. H. (1994). Direct gel chromatographic characterization and quantification of marine dissolved organic carbon using high-sensitivity DOC detection. Environmental Science & Technology, 28, 1194–1197.CrossRefGoogle Scholar
  11. Jaffe, R., Boyer, J. N., Lu, X., et al. (2004). Source characterization of dissolved organic matter in a subtropical mangrove-dominated estuary by fluorescence analysis. Marine Chemistry, 84, 195–210.CrossRefGoogle Scholar
  12. Kutter, J. P. (2000). Current developments in electrophoretic and chromatographic separation methods on microfabricated devices. Trends in Analytic Chemistry, 19, 352–363.CrossRefGoogle Scholar
  13. Schmitt-Kopplin, P., Freitag, D., Kettrup, A., et al. (1999). Capillary zone electrophoretic studies on Norwegian surface water natural organic matter. Environment International, 25, 259–274.CrossRefGoogle Scholar
  14. Specht, C., & Frimmel, F. H. (2000). Specific interactions of organic substances in size-exclusion chromatography. Environmental Science & Technology, 34, 2361–2366.CrossRefGoogle Scholar
  15. Tian, D. M., Deng, G. C., Zhang, Y. Y., et al. (2002). Removal chloride ion by adsorption and microwave digestion in the determination of chemical oxygen demand. Chinese Journal of Analytical Chemistry, 30, 522–526.Google Scholar
  16. Wakida, S., Chiba, A., Matsuda, T., et al. (2001). High-throughput characterization for organic pollutants in environmental waters using a capillary electrophoresis chip. Electrophoresis, 22, 3505–3508.CrossRefGoogle Scholar
  17. Wakida, S., Takeda, S., Miyazaki, S., et al. (1997). Capillary gel electrophoresis for characterization of dissolved organic substances in environmental waters. Bunseki Kagaku, 46, 483–490.Google Scholar
  18. Zeng, B. W. (1979). Environmental analytical chemistry (pp. 67–78). Changsha: Hunan Science and Technology Press.Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  1. 1.Department of ChemistryChangzhi CollegeChangzhiChina
  2. 2.National Institute of Advanced Industrial Science and TechnologyOsakaJapan

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