Assessment of pesticide residues in freshwater areas affected by rice paddy effluents in Southern Japan

  • Nathaniel Añasco
  • Seiichi Uno
  • Jiro Koyama
  • Tatsuro Matsuoka
  • Naoya Kuwahara


Pesticide residues in five freshwater areas that are directly affected by rice paddy effluents in southern Japan were measured to determine their maximum concentrations and temporal variations. Water samples were collected every week during the 2005 rice planting season in Kagoshima Prefecture and stations were established in Amori River, Sudo River, Nagaida River (that drains into the bigger Kotsuki River), rice paddy drainage canal, and wastewater reservoir (that collects effluents from rice paddy fields). Of the 14 target pesticides examined, a total of 11 were detected in all stations. Mefenacet, fenobucarb, and flutolanil were the three pesticides with the highest maximum concentrations and were also detected frequently. Analysis of temporal variations of pesticides showed that herbicides had relatively higher concentrations in the earlier stages of the rice planting season, while insecticides and fungicides had relatively higher concentrations at the later stages. There was no significant difference among stations with regards to the temporal patterns of the top three pesticides. The calculated toxic units were less than 1 in all stations, implying low or negligible environmental risk of pesticides detected to freshwater organisms.


Pesticides Rice paddy canal Rivers Wastewater reservoir Concentrations Temporal variation 


  1. Anderson, B. S., Hunt, J. W., Phillips, B. M., Nicely, P. A., de Vlaming, V., Connor, V., et al. (2003). Integrated assessment of the impacts of agricultural drainwater in the Salinas River (California, USA). Environmental Pollution, 124, 523–532. doi:10.1016/S0269-7491(03)00012-5.CrossRefGoogle Scholar
  2. Belfroid, A. C., van Drunen, M., Beek, M. A., Schrap, S. M., VanGestel, C. A. M., & Van Hattum, B. (1998). Relative risks of transformation products of pesticides for aquatic ecosystems. The Science of the Total Environment, 222, 167–183. doi:10.1016/S0048-9697(98)00298-8.CrossRefGoogle Scholar
  3. Cedergreen, N., & Streibig, J. C. (2005). The toxicity of herbicides to non-target aquatic plants and algae: Assessment of predictive factors and hazard. Pest Management Science, 61, 1152–1160. doi:10.1002/ps.1117.CrossRefGoogle Scholar
  4. Comoretto, L., Arfib, B., & Chiron, S. (2007). Pesticides in the Rhone river delta (France): Basic data for a field-based exposure assessment. The Science of the Total Environment, 380, 124–132. doi:10.1016/j.scitotenv.2006.11.046.CrossRefGoogle Scholar
  5. Derbalah, A. S. H., Nakatani, N., & Sakugawa, H. (2003). Distribution, seasonal pattern, flux and contamination source of pesticides and nonylphenol residues in Kurose River water, Higashi-Hiroshima, Japan. Geochemical Journal, 37, 217–232.Google Scholar
  6. Fushiwaki, Y., Tase, N., Kotoda, K., & Urano, K. (1994). Behavior of fungicide pentachloronitrobenzene and intermediates in an intensive farming area in Japan. Japanese Journal of Toxicology and Environmental Health, 40, 39–48.Google Scholar
  7. GeNii National Institute of Informatics Scholarly and Academic Information Portal (2007).
  8. Gil, Y., & Sinfort, C. (2005). Emission of pesticides to the air during sprayer application: A bibliographic review. Atmospheric Environment, 39, 5183–5193. doi:10.1016/j.atmosenv.2005.05.019.CrossRefGoogle Scholar
  9. Hatakeyama, S., & Shiraishi, H. (1998). Biomonitoring with shrimp to detect seasonal change in river water toxicity. Environmental Toxicology and Chemistry, 17, 687–694. doi:10.1897/1551-5028(1998)017<0687:BWSTDS>2.3.CO;2.CrossRefGoogle Scholar
  10. Hatakeyama, S., Fukushima, S., Kasai, F., & Shiraishi, H. (1994). Assessment of herbicide effects on algal production in the Kokai River (Japan) using a model stream and Selenastrum bioassay. Ecotoxicology (London, England), 3, 143–156. doi:10.1007/BF00143411.Google Scholar
  11. Hatakeyama, S., Inoue, T., Suzuki, K., Sugaya, Y., & Kasuga, S. (1999). Assessment of overall herbicide effects on growth of duckweed in a flow through aquarium carrying pesticide polluted river water. Japanese Journal of Environmental Toxicology, 2, 65–75.Google Scholar
  12. Inoue, T., Ebise, S., Numabe, A., Nagafuchi, O., & Matsui, Y. (2002). Runoff characteristics of particulate pesticides in a river from paddy fields. Water Science and Technology, 45, 121–126.Google Scholar
  13. Itagaki, N., Nagafuchi, O., Takimoto, K., & Okada, M. (2000). Fate of pesticides in a shallow reservoir. Water Science and Technology, 42, 217–222.Google Scholar
  14. Japan Statistical Bureau (2007).
  15. Kanazawa, J. (1994). Environmental science of pesticides [in Japanese] (pp. 141–156). Japan: Godo-Shuppan.Google Scholar
  16. Kawakami, T., Eun, H., Arao, T., Endo, S., Ueji, M., Tamura, K., et al. (2006). Concentration and loading of several pesticides in water, suspended solids and sediment during ordinary water discharge in Sugao Marsh, Ibaraki Prefecture, Japan. Journal of Pesticide Science, 31, 6–13. doi:10.1584/jpestics.31.6.CrossRefGoogle Scholar
  17. Kawakami, T., Ishizaka, M., Ishii, Y., Eun, H., Miyazaki, J., Tamura, K., et al. (2005). Concentration and distribution of several pesticides applied to paddy fields in water and sediment, from Sugao Marsh, Japan. Bulletin of Environmental Contamination and Toxicology, 74, 954–961. doi:10.1007/s00128-005-0672-5.CrossRefGoogle Scholar
  18. Kegley, S. E., Hill, B. R., Orme, S., & Choi, A. H. (2008). PAN Pesticide Database, Pesticide Action Network, North America (San Francisco, CA).
  19. Kibe, K., Takahashi, M., Kameya, T., & Urano, K. (2000). Adsorption equilibriums of principal herbicides on paddy soils in Japan. The Science of the Total Environment, 263, 115–125. doi:10.1016/S0048-9697(00)00671-9.CrossRefGoogle Scholar
  20. Nakano, Y., Miyazaki, A., Yoshida, T., Ono, K., & Inoue, T. (2004). A study on pesticide runoff from paddy fields to a river in rural region—1: Field survey of pesticide runoff in the Kozakura River, Japan. Water Research, 38, 3017–3022. doi:10.1016/j.watres.2004.02.013.CrossRefGoogle Scholar
  21. Nohara, S., & Iwakuma, T. (1996a). Pesticide residues in water and an aquatic plant, Nelumbo nucifera, in a river mouth at Lake Kasumigaura, Japan. Chemosphere, 33, 1409–1416. doi:10.1016/0045-6535(96)00258-5.CrossRefGoogle Scholar
  22. Nohara, S., & Iwakuma, T. (1996b). Residual pesticides and their toxicity to freshwater shrimp in the littoral and pelagic zone of Lake Kasumigaura, Japan. Chemosphere, 33, 1417–1424. doi:10.1016/0045-6535(96)00259-7.CrossRefGoogle Scholar
  23. Ntiamoah, A., & Afrane, G. (2008). Environmental impacts of cocoa production and processing in Ghana: Life cycle assessment approach. Journal of Cleaner Production, 16, 1735–1740. doi:10.1016/j.jclepro.2007.11.004.CrossRefGoogle Scholar
  24. Numabe, A., & Nagahora, S. (2006). Estimation of pesticide runoff from paddy fields to rural rivers. Water Science and Technology, 53, 139–146. doi:10.2166/wst.2006.047 .CrossRefGoogle Scholar
  25. Okamura, H., Omori, M., Luo, R., Aoyama, I., & Liu, D. (1999). Application of short-term bioassay guided chemical analysis for water quality of agricultural land run-off. The Science of the Total Environment, 234, 223–231. doi:10.1016/S0048-9697(99)00263-6.CrossRefGoogle Scholar
  26. Okamura, H., Piao, M., Aoyama, I., Sudo, M., Okubo, T., & Nakamura, M. (2002). Algal growth inhibition by river water pollutants in the agricultural area around Lake Biwa, Japan. Environmental Pollution, 117, 411–419. doi:10.1016/S0269-7491(01)00196-8.CrossRefGoogle Scholar
  27. Organization for Research and Communication on Environmental Risk of Chemicals (2007).
  28. Parveen, S., Kohguchi, T., Biswas, M., & Nakagoshi, N. (2005a). Predicting herbicide concentrations in paddy water and runoff to the river basin. Journal of Environmental Sciences (China), 17, 631–636.Google Scholar
  29. Parveen, S., Kohguchi, T., Shimazawa, H., & Nakagoshi, N. (2005b). Measuring of some selected herbicides in paddy surface waters in the Saijo Basin, Western Japan. Agronomy Sustainable and Development, 25, 55–61. doi:10.1051/agro:2004055.CrossRefGoogle Scholar
  30. Sakai, M. (2003). Investigation of pesticides in rainwater at Isogo Ward of Yokohama. Journal of Health Science, 49, 221–225. doi:10.1248/jhs.49.221.CrossRefGoogle Scholar
  31. Sato, H. (2001). The current state of paddy agriculture in Japan. Irrigation and Drainage, 50, 91–99. doi:10.1002/ird.10.CrossRefGoogle Scholar
  32. Scholz, N. L., & Hopkins, W. A. (2006). Ecotoxicology of anticholinesterase pesticides: Data gaps and research challenges. Environmental Toxicology and Chemistry, 25, 1185–1186. doi:10.1897/05-699.1.CrossRefGoogle Scholar
  33. Steen, R. J. C. A., van der Vaart, J., Hiep, M., VanHattum, B., Cofino, W. P., & Brinkman, U. A. T. (2001). Gross fluxes and estuarine behavior of pesticides in the Scheldt Estuary (1995–1997). Environmental Pollution, 115, 65–79. doi:10.1016/S0269-7491(01)00085-9 .CrossRefGoogle Scholar
  34. Sudo, M., Kawachi, T., Hida, Y., & Kunimatsu, T. (2004). Spatial distribution and seasonal changes of pesticides in Lake Biwa, Japan. Limnology, 5, 77–86. doi:10.1007/s10201-003-0115-0.CrossRefGoogle Scholar
  35. Sudo, M., Kunimatsu, T., & Okubo, T. (2002). Concentration and loading of pesticide residues in Lake Biwa basin (Japan). Water Research, 36, 315–329. doi:10.1016/S0043-1354(01)00196-8.CrossRefGoogle Scholar
  36. Sudo, M., Okubo, T., & Kaneki, R. (2005). Paddy herbicide inputs in the entire river inflow reaching Lake Biwa, Japan. Limnology, 6, 91–99. doi:10.1007/s10201-005-0145-x.CrossRefGoogle Scholar
  37. Sudo, M., Nishino, M., & Okubo, T. (2006). Decrease in herbicide concentrations and affected factors in lagoons located around Lake Biwa. Water Science and Technology, 53, 131–138. doi:10.2166/wst.2006.046.CrossRefGoogle Scholar
  38. Suzuki, S., Otani, T., Iwasaki, S., Ito, K., Omura, H., & Tanaka, Y. (2003). Monitoring of 15 pesticides in rainwater in Utsunomiya, eastern Japan, 1999–2000. Journal of Pesticide Science, 28, 1–7. doi:10.1584/jpestics.28.1.CrossRefGoogle Scholar
  39. Tada, M., & Hatakeyama, S. (2000). Chronic effects of an insecticide, fenobucarb, on the larvae of two mayflies, Epeorus latifolium and Baetis thermicus, in model streams. Ecotoxicology (London, England), 9, 187–195. doi:10.1023/A:1008990604166.Google Scholar
  40. Tanabe, A., Mitobe, H., Kawata, K., Yasuhara, A., & Shibamoto, T. (2001). Seasonal and spatial studies on pesticide residues in surface waters of the Shinano River in Japan. Journal of Agricultural and Food Chemistry, 49, 3847–3852. doi:10.1021/jf010025x.CrossRefGoogle Scholar
  41. Thomson Scientific ISI Web of Knowledge (2007).
  42. Ueji, M., & Inao, K. (2001). Rice paddy field herbicides and their effects on the environment and ecosystems. Weed Biology and Management, 1, 71–79. doi:10.1046/j.1445-6664.2001.00002.x.CrossRefGoogle Scholar
  43. Uno, S., Shiraishi, H., Hatakeyama, S., Otsuki, A., & Koyama, J. (2001). Accumulative characteristics of pesticide residues in organs of bivalves (Anodonta woodiana and Corbicula leana) under natural conditions. Archives of Environmental Contamination and Toxicology, 40, 35–47. doi:10.1007/s002440010146.CrossRefGoogle Scholar
  44. Yamaguchi, Y., Fukushima, M., Fujita, T., Yamamoto, T., & Yamada, A. (1992). Distribution and seasonal variation of pesticide residues in Yodo River basin, Japan. Water Science and Technology, 25, 61–68.Google Scholar
  45. Zhang, X. Y., Liu, X. M., Lou, Y. Z., & Zhang, M. H. (2008). Evaluation of water quality in an agricultural watershed as affected by almond pest management practices. Water Research, 42, 3685–3696. doi:10.1016/j.watres.2008.05.018.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Nathaniel Añasco
    • 1
  • Seiichi Uno
    • 2
  • Jiro Koyama
    • 2
  • Tatsuro Matsuoka
    • 2
  • Naoya Kuwahara
    • 2
  1. 1.United Graduate School of Agricultural SciencesKagoshima UniversityKagoshimaJapan
  2. 2.Education and Research Center for Marine Resources and EnvironmentKagoshima University Faculty of FisheriesKagoshima CityJapan

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