Environmental Monitoring and Assessment

, Volume 136, Issue 1–3, pp 299–306 | Cite as

Effect of heavy metals on microbial biomass and activities in century old landfill soil

  • P. Bhattacharyya
  • A. Mitra
  • K. Chakrabarti
  • D. J. Chattopadhyay
  • A. Chakraborty
  • K. Kim


A study was conducted to determine the effect of metals on soil microbial biomass and activities in landfill soils as well as normal background soil. The microbial biomass and activities were consistently higher in the landfill soils than in the background soil. Significant positive correlations existed between the microbial parameters and soil organic carbon. The landfill soils contained higher concentrations of metals (iron, manganese, copper, cadmium, lead and zinc) than did the background soil. Microbial parameters were negatively correlated with the metals, with inhibition increasing with the bioavailability of the metals. It is suggested that the metals affected microbial biomass and activities by behaving synergistically or additively with each other. Although the landfill soils had higher microbial biomass and activities than the background soil, due to higher organic matter content, the ratios of microbial parameters/organic carbon indicated that inhibition of microbial growth and activities had occurred due to metal stress.


Heavy metal Microbial biomass Respiration Fluorescein diacetate activity Landfill soil 


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  1. Adriano, D. C. (1986). Trace elements in the terrestrial environment. Berlin Heidelberg New York: Springer.Google Scholar
  2. Alef, K. (1995). Soil respiration. In K. Alef & P. Nannipieri (Eds.), Methods in applied soil microbiology and biochemistry. London, UK: Academic.Google Scholar
  3. Anderson, J. P. E., & Domsch, K. H. (1978). A physiological method for the quantitative measurement of biomass in soils. Soil Biology and Biochemistry, 10, 215–221.CrossRefGoogle Scholar
  4. Baath, E. (1989). Effects of heavy metals in soil on microbial processes and populations. Water, Air and Soil Pollution, 7, 335–379.CrossRefGoogle Scholar
  5. Badgett, R. D., & Saggar, S. (1994). Effects of heavy metal contamination on the short-term decomposition of labeled [14C] glucose in a pasture soil. Soil Biology and Biochemistry, 26, 727–733.CrossRefGoogle Scholar
  6. Beckett, P. H. T., & Davis, R. D. (1978). The additivity of the toxic effects of Cu, Ni and Zn in young barley. New Phytolology, 81, 155–173.CrossRefGoogle Scholar
  7. Bhattacharyya, P., Chakrabarti, K., & Chakraborty, A. (2005). Microbial biomass and enzyme activities in submerged rice soil amended with municipal solid waste compost and decomposed cow manure. Chemosphere, 60, 310–318.CrossRefGoogle Scholar
  8. Bhattacharyya, P., Pal, R., Chakraborty, A., & Chakrabarti, K. (2001). Microbial biomass and its activities of a laterite soil amended with municipal solid waste compost. Journal of Agronomy and Crop Science, 187, 207–211.CrossRefGoogle Scholar
  9. Brookes, P. C. (1995). The use of microbial parameters in monitoring soil pollution. Biology and Fertility of Soils, 19, 269–279.CrossRefGoogle Scholar
  10. Brookes, P. C. (2000). Changes in soil microbial properties as indicators of adverse effects of heavy metals. Memorie di Scienze Fisiche e Naturali 118, 24, 205–227.Google Scholar
  11. Chander, K., Dyckmans, J., Joergensen, R. G., Meyer, B., & Raubuch, M. (2004). Different sources of heavy metals and their long-term effects on soil microbial properties. Biology and Fertility of Soils, 34, 241–247.CrossRefGoogle Scholar
  12. Cheng, W., Zhang, Q., Coleman, D. C., Carrol, C. R., & Hoffman, C. A. (1996). Is available carbon limiting microbial respiration in the rhizosphere? Soil Biology and Biochemistry, 28, 1283–1288.CrossRefGoogle Scholar
  13. Dick, R. P. (1994). Soil enzyme activities as indicators of soil quality. In J. W. Doran, D. C. Coleman, D. F. Bezdicek, & B. A. Stewart (Eds.), Defining soil quality for sustainable environment. Special Pub. 35. Madison, WI: Soil Science Society of America.Google Scholar
  14. Ghosh, A. K., Bhattacharyya, P., & Pal, R. (2004). Effect of arsenic contamination on microbial biomass and its activities in arsenic contaminated soils of Gangetic West Bengal, India. Environment International, 30, 491–499.CrossRefGoogle Scholar
  15. Hiroki, M. (1992). Effects of heavy metal contamination on soil microbial population. Soil Science & Plant Nutrition, 38, 141–147.Google Scholar
  16. Jenkinson, D. S., & Ladd, J. N. (1981). Microbial biomass in soil: Measurement and turnover. In E. A. Paul & J. N. Ladd (Eds.), Soil biochemistry, vol. 5. New York: Marcel Dekker.Google Scholar
  17. Lindsay, W. L., & Norvell, K. A. (1978). Development of a DTPA soil test for zinc, iron, manganese and copper. Soil Science Society of America Journal, 42, 421–428.CrossRefGoogle Scholar
  18. Ma, Y. B., & Uren, N. C. (1998). Transformation of heavy metals added to soil: Application of a new sequential extraction procedure. Geoderma, 84, 157–168.CrossRefGoogle Scholar
  19. Mitra, A., Bhattacharyya, P., Chakrabarti, K., Chattopadhyay, D. J., & Chakraborty, A. (2003). Physico-chemical properties, heavy metals and their relations in cultivated landfill soils dumped with municipal solid wastes. Archives of Agronomy and Soil Science, 49, 163–170.CrossRefGoogle Scholar
  20. Nannipieri, P., Gregos, S., & Ceccanti, B. (1990). Ecological significance of the biological activity in soil. In J. L. Smith & E. A. Paul (Eds.), Soil biochemistry, vol. 6. New York: Marcel Dekker.Google Scholar
  21. Nelson, D. W., & Sommers, L. E. (1982). Total carbon, organic carbon and organic matter. In A. L. Page, R. H. Miller, & D. R. Keeney (Eds.), Methods of soil analysis. Part 2. (2nd ed.). Madison, WI: Soil Science Society of America.Google Scholar
  22. Page, A. L., Miller, R. H., & Keeney, D. R. (1982). Methods of soil analysis. Part 2. (2nd ed.). Madison, WI: Soil Science Society of America.Google Scholar
  23. Pankhurst, C. E., Hawke, B. G., McDonald, H. J., Kirby, C. A., Buckerfield, J. C., Michelsen, P., et al. (1995). Evaluation of soil biological properties as potential bioindicators of soil health. Australian Journal of Experimental Agriculture, 35, 1015–1028.CrossRefGoogle Scholar
  24. Piper, C. S. (1966). Soil and plant analysis. Bombay, India: Maver.Google Scholar
  25. Schnurer, J., & Rosswall, T. (1982). Fluorescein diacetate hydrolysis as a measure of total microbial activity in soil and litter. Applied Environmental Microbiology, 43, 1256–1261.Google Scholar
  26. Stratton, M. L., Barker, A. V., & Rechcigl, J. E. (1995). Compost. In J. E. Rechcigl (Ed.), Soil amendments and environmental quality. New York: Lewis.Google Scholar
  27. Vance, E. D., Brookes, P. C., & Jenkinson, D. S. (1987). An extraction method for measuring soil microbial biomass C. Soil Biology and Biochemistry, 19, 703–707.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • P. Bhattacharyya
    • 1
  • A. Mitra
    • 2
  • K. Chakrabarti
    • 2
  • D. J. Chattopadhyay
    • 3
  • A. Chakraborty
    • 4
  • K. Kim
    • 1
  1. 1.BK21 Advanced Geo-Environment Research Team, School of Environmental EngineeringKunsan National UniversityKunsanSouth Korea
  2. 2.Institute Agricultural ScienceCalcutta UniversityCalcuttaIndia
  3. 3.B.C. Guha Centre for Genetic Engineering and BiotechnologyCalcutta UniversityCalcuttaIndia
  4. 4.Department of AgronomyBidhan Chandra Krishi ViswavidyalayaNadiaIndia

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