Enumeration of Legionella pneumophila in cooling tower water systems



Legionella pneumophila, the causative agent of Legionnaires’ disease, is known to colonise and frequently grow in cooling tower waters. Disease is acquired by inhaling aerosol contaminated by legionellae. Determination of the count of Legionella pneumophila in cooling tower waters may, therefore, be useful for risk assessment. In our survey, 103 water samples from 50 cooling towers were examined over a five-year period to indicate the seasonal distribution and the ecology of L. pneumophila, as regards temperature and pH. L. pneumophila serogroup 1 was found in 44% of the isolated strains, which is primarily responsible for the majority of Legionnaires’ disease. The large majority of examined towers had levels of L. pneumophila in the high-risk category. These cooling towers have been linked to many outbreaks of Legionnaires’ disease.


aerosol cooling tower Legionnaires’ disease Legionella pneumophila risk assessment 


  1. Atlas, R. M. 1999From environmental habitats to disease pathology, detection and controlEnviron. Microbiol.1283293Google Scholar
  2. Benson, R. F., Fields, B. S. 1998Classification of the genus LegionellaSem. Respiratory Infect.139099Google Scholar
  3. Bentham, R. H. 2000Routine sampling and the control of Legionella spp. in cooling tower water systemsCurr. Microbiol.41271275Google Scholar
  4. Bhopal, R. S, Fallon, R. J., Buist, E. C., Black, R. J., Urquhart, J. D. 1991Proximity of the home to a cooling tower and risk of non-outbreak legionnaires’ diseaseBr. Med. J.302378383CrossRefGoogle Scholar
  5. Bopp, C. A., Sumner, J. W., Morris, G. K., Wells, J. G. 1981Isolation of Legionella ssp. from environmental water samples by low-pH treatment and use of a selective mediumJ. Clin. Bacteriol.13714719Google Scholar
  6. Bosch, X. 2001Legionnaires’ outbreak in Spanish town may be largest everLancet358220221Google Scholar
  7. Broadbent, C. R.: 1993, ‘Legionella in cooling towers: practical research, design, treatment, and control guidelines. Legionella: Current status and emerging perspectives’, in J. M. Barbaree, R. F., Breiman, and A. P. Dufour (eds), Am. Society for Microbiol., Washington, 217–221.Google Scholar
  8. Dennis, P. J. L. 1988Isolation of Legionellae from Environmental SpecimensHarrison, T. G.Taylor, A. G. eds. A Laboratory Manual for LegionellaJ. Wiley & SonsChichester3144Google Scholar
  9. Garbe, L. P., Davis, J. B., Weisfeld, J. S., Markowitz, L., Miner, P., Garrity, F., Barbaree, J. M., Reingold, A. L. 1985Nosocomial legionnaires’ disease: epidemiologic demonstration of cooling towers as a sourceJAMA254521524Google Scholar
  10. Ishimatsu, S., Miyamoto, H., Hori, H., Tanaka, I., Yoshida, S. 2001Sampling and detection of Legionella pneumophila aerosols generated from an industrial cooling towerAnn. Occu. Hyg.6421427Google Scholar
  11. Lin, Y. E., Vidic, R. D., Stout, J. E., Yu, V.L. 1998Legionella in water distribution systemsAWWA90112121Google Scholar
  12. Martinelli, F., Caruso, A., Moschini, L., Turano, A., Scarcella, C., Speziani, F. 2000A comparison of Legionella pneumophila in hot water tanks and instantaneous devices in domestic, nosocomial, and community environmentsCurr. Microbiol.41374376Google Scholar
  13. Miller, R. D. and Kenepp, K. A.: 1993, ‘Risk Assessments for Legionnaires’ Disease Based on Routine Surveillance of Cooling Towers of Legionellae. Legionella: Current Status and Emerging Perspectives’, in J. M. Barbaree, R. F. Breiman and A. P. Dufour (eds), American Society for Microbiology, Washington, 40–43.Google Scholar
  14. Sabria, M., Yu, V. L. 2002Hospital-acquired legionellosis: solutions for a preventable infectionLancet2368373CrossRefGoogle Scholar
  15. Sedgwick, A. K., Tilton, R. C. 1982Identification of Legionella pneumophila by latex agglutinationJ. Clin. Microbiol.22365369Google Scholar
  16. Yamamoto, H., Sugiura, M., Kusunoki, S., Ezaki, T., Ikedo, M., Yabuuchi, E. 1992Factors stimulating propagation of Legionellae in cooling tower waterAppl. Environ. Microbiol.5813941397Google Scholar
  17. Yu, V. L. 1993Could aspiration be the major mode of transmission for Legionella Am. J. Med.951315Google Scholar

Copyright information

© Springer 2005

Authors and Affiliations

  • Irfan Türetgen
    • 1
  • Esra Ilhan Sungur
    • 1
  • Aysin Cotuk
    • 1
  1. 1.Department of Biology, Faculty of ScienceIstanbul UniversityIstanbulTurkey

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