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Metabolites from symbiotic bacteria of entomopathogenic nematodes have antimicrobial effects against Pythium myriotylum

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Abstract

Pythium myriotylum is a destructive soil-borne phytopathogen, causing yield losses in ginger and many other crops. Environmental and regulatory concerns drive the need to find biological alternatives to conventional pesticides used to manage P. myriotylum. Trans-cinnamic acid (TCA) alone, and fermentation broth from strains of symbiotic bacteria of eight species of entomopathogenic nematodes alone, and in combination with TCA, were tested for their effect on zoospore germination and mycelial growth of P. myriotylum. TCA significantly inhibited mycelial growth. Fermentation broths from seven of the eight strains of symbiotic bacteria directly inhibited mycelial growth, especially those isolated from Steinernema feltiae (strain SN) and S. riobrave (strain 7–12). Moreover, adding TCA significantly increased the inhibitory effect on mycelial growth of the fermentation broths of seven of the strains tested. All bacteria fermentation broths showed inhibitory effects on zoospore germination. However, TCA alone was not inhibitory to zoospore germination but was inhibitory to mycelial growth. Antimicrobial effects on mycelial growth and zoospore germination were proportional to the concentration of symbiotic bacteria isolated from S. feltiae (strain SN). These results show that TCA and symbiotic bacteria of entomopathogenic nematodes may have potential to provide biorational control of P. myriotylum.

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Acknowledgments

This work was jointly supported by the National Key R&D Program of China (2017YFE0130400 and 2018YFD0201002) and the Natural Science Foundation of China (31470495 and 31170412), the China National Tobacco Corporation (110202001034-LS 03), the Yunnan Provincial Company of National Tobacco Corporation (2017YN15 and 2014YN21), the Fundamental Research Funds for the Central Universities to Dr. Ruan and the 111 project (B08011). We gratefully acknowledge Dr. Liu Aixin for providing the information on Pythium myriotylum identification. We also gratefully acknowledge anonymous reviewers for valuable comments on the manuscript.

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Authors and Affiliations

  1. College of Life Sciences, Nankai University, Tianjin, 300071, China

    Shaojie Shan, Yang Li, Cuiqing Huang, Bingjiao Sun, Chaoying Chen, Xianqin Wei, Guangshuang Shen & Weibin Ruan

  2. Department of Terrestrial Ecology, Netherlands Institute of Ecology (NIOO-KNAW), P. O. Box 50, 6700 AB, Wageningen, The Netherlands

    Haikun Ma

  3. Tobacco Company, Yuxi, 653100, Yunnan, China

    Xinghui Gu

  4. Linyi Kingfarm Agricultural Service Co., Ltd, 276700, Linyi, China

    Zhensheng Jiang

  5. USDA-ARS, SEFTNRL, Byron, GA, 31008, USA

    David Shapiro-Ilan

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  1. Shaojie Shan
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Correspondence to Weibin Ruan.

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Shan, S., Ma, H., Li, Y. et al. Metabolites from symbiotic bacteria of entomopathogenic nematodes have antimicrobial effects against Pythium myriotylum. Eur J Plant Pathol 158, 35–44 (2020). https://doi.org/10.1007/s10658-020-02053-2

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