Skip to main content
SpringerLink
Log in

Complete genomic sequence of Noni mosaic virus (NoMV) associated with a mosaic disease in Morinda citrifolia L.

  • Published:
European Journal of Plant Pathology Aims and scope Submit manuscript

Abstract

An outbreak of a virus-like disease has caused severe damage to noni plants (Morinda citrifolia L.) in Xishuangbanna area of Yunnan province in southwest China since 2015. The diseased plants displayed typical mosaic symptom with light and dark green patches on leaves. Flexuous filamentous virus particles of about 800 nm in length were observed from the leaf saps by transmission electron microscope. Illuemina transcriptomic sequencing further revealed the presence of a potyvirus and its near complete genome was obtained from de novo assembly. The complete genome of 9659 nts was obtained by Sanger sequencing of eight amplicons generate by RT-PCR and 5′ and 3’ RACE. BLASTp analysis of the polyprotein sequence showed that the virus was most closely related to Tobacco vein banding mosaic virus (TVBMV), but these two viruses only shared 50.7% amino acid sequence similarity. Both phylogenetic analyses of the polyprotein and CP amino acid sequences indicated that this virus is a member of genus Potyvirus. However, the low sequence homology with all known potyviruses established this virus as a new species in the genus, tentatively named as Noni mosaic virus (NoMV). Our field surveys showed that 100% of the symptomatic samples and 28.57% of the asymptomatic samples were infected with this novel potyvirus. Aphids collected from diseased leaves were also detected carrying the virus and aphid transmission test confirmed it can transmit the NoMV. In summary, our data indicated that a novel species of potyvirus, NoMV, is prevalent in Yunnan, China and is associated with an emerging mosaic disease on M. citrifolia.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Adams, M. J., Antoniw, J. F., & Fauquet, C. M. (2005). Molecular criteria for genus and species discrimination within the family Potyviridae. Archives of Virology, 150, 459–479.

    Article  CAS  Google Scholar 

  • Ahmad, A. N., Daud, Z. A. M., & Ismail, A. (2016). Review on potential therapeutic effect of Morinda Citrifolia, L. Current Opinion in Food Science, 8, 62–67.

    Article  Google Scholar 

  • Anders, S., Pyl, P. T., & Huber, W. (2015). HTSeq--a Python framework to work with high-throughput sequencing data. Bioinformatics, 31(2), 166–169.

    Article  CAS  Google Scholar 

  • Cao, M., Zhang, S., Li, M., Liu, Y., Dong, P., Li, S., Kuang, M., Li, R., & Zhou, Y. (2019). Discovery of four novel viruses associated with flower yellowing disease of green Sichuan pepper (Zanthoxylum Armatum) by virome analysis. Viruses, 11(8), 696.

    Article  CAS  Google Scholar 

  • Cui, X., Yaghmaiean, H., Wu, G., Wu, X., Chen, X., Thorn, G., & Wang, A. (2017). The C-terminal region of the Turnip mosaic virus P3 protein is essential for viral infection via targeting P3 to the viral replication complex. Virology, 510, 147–155.

    Article  CAS  Google Scholar 

  • Diaz-Lara, A., Navarro, B., Di Serio, F., Stevens, K., Hwang, M. S., Kohl, J., Vu, S. T., Falk, B. W., Golino, D., & Al Rwahnih, M. (2019). Two novel negative-sense RNA viruses infecting grapevine are members of a newly proposed genus within the family Phenuiviridae. Viruses, 11(8), 685.

    Article  CAS  Google Scholar 

  • Gan, B. C., & He, M. J. (2004). The planting and use of Morinda citrifolia. Forest By-Product and Speciality in China, 2, 7–9.

    Google Scholar 

  • Gong, D., Wang, J. H., Lin, Z. S., Zhang, S. Y., Zhang, Y. L., Yu, N. T., Xiong, Z., & Liu, Z. X. (2011). Genomic sequencing and analysis of Chilli ringspot virus, a novel potyvirus. Virus Genes, 43(3), 439–444.

    Article  CAS  Google Scholar 

  • Haas, B. J., Papanicolaou, A., Yassour, M., Grabherr, M., Blood, P. D., Bowden, J., Couger, M. B., Eccles, D., Li, B., Lieber, M., MacManes, M. D., Ott, M., Orvis, J., Pochet, N., Strozzi, F., Weeks, N., Westerman, R., William, T., Dewey, C. N., Henschel, R., LeDuc, R. D., Friedman, N., & Regev, A. (2013). De novo transcript sequence reconstruction from RNA-seq using the trinity platform for reference generation and analysis. Nature Protocols, 8(8), 1494–1512.

    Article  CAS  Google Scholar 

  • Hall, T. A. (2013). BioEdit, version 7.2.5; software for biological sequence alignment. Carlsbad, CA, USA.: Ibis Biosciences.

    Google Scholar 

  • Hao, X., Zhang, W., Zhao, F., Liu, Y., Qian, W., Wang, Y., Wang, L., Zeng, J., Yang, Y., & Wang, X. (2018). Discovery of plant viruses from tea plant (Camellia sinensis (L.) O. Kuntze) by metagenomic sequencing. Front Microbiology, 9, 2175.

    Article  Google Scholar 

  • Huang, C. H., Tai, C. H., Lin, R. S., Chang, C. J., & Jan, F. J. (2019). Biological, pathological, and molecular characteristics of a new potyvirus, Dendrobium chlorotic mosaic virus, infecting Dendrobium orchid. Plant Disease, 103(7), 1605–1612.

    Article  Google Scholar 

  • López-Moya, J. J., Wang, R. Y., & Pirone, T. P. (1999). Context of the coat protein DAG motif affects potyvirus transmissibility by aphids. The Journal of General Virology, 80(Pt 12), 3281–3288.

    Article  Google Scholar 

  • Plisson, C., Drucker, M., Blanc, S., German-Retana, S., Le, G. O., Thomas, D., & Bron, P. (2003). Structural characterization of HC-pro, a plant virus multifunctional protein. The Journal of Biological Chemistry, 278(26), 23753–23761.

    Article  CAS  Google Scholar 

  • Qian, Y., Xu, Y., Zhou, Q., & Zhou, X. (2014). Application of next-generation sequencing technology for plant virus identification. Scientia Sinica Vitae, 44(4), 351–363.

    Article  Google Scholar 

  • Revers, F., & Garcã-A, J. A. (2015). Molecular biology of potyviruses. Advances in Virus Research, 92, 101–199.

    Article  CAS  Google Scholar 

  • Rott, M. E., Kesanakurti, P., Berwarth, C., Rast, H., Boyes, I., Phelan, J., & Jelkmann, W. (2018). Discovery of negative-sense RNA viruses in trees infected with apple rubbery wood disease by next-generation sequencing. Plant Disease, 102(7), 1254–1263.

    Article  CAS  Google Scholar 

  • Seo, J. K., Kang, S. H., Seo, B. Y., Jung, J. K., & Kim, K. H. (2010). Mutational analysis of interaction between coat protein and helper component-proteinase of Soybean mosaic virus involved in aphid transmission. Molecular Plant Pathology, 11(2), 265–276.

    Article  CAS  Google Scholar 

  • Shan, H., Pasin, F., Tzanetakis, I. E., Simón-Mateo, C., García, J. A., & Rodamilans, B. (2018). Truncation of a P1 leader proteinase facilitates potyvirus replication in a non-permissive host. Molecular Plant Pathology, 19(6), 1504–1510.

    Article  CAS  Google Scholar 

  • Sheveleva, A., Kudryavtseva, A., Speranskaya, A., Belenikin, M., Melnikova, N., & Chirkov, S. (2013). Complete genome sequence of a novel Plum pox virus strain W isolate determined by 454 pyrosequencing. Virus Genes, 47(2), 385–388.

    Article  CAS  Google Scholar 

  • Stenger, D. C., Hein, G. L., Gildow, F. E., Horken, K. M., & French, R. (2005). Plant virus HC-pro is a determinant of eriophyid mite transmission. Journal of Virology, 79(14), 9054–9061.

    Article  CAS  Google Scholar 

  • Tamura, K., Stecher, G., Peterson, D., Filipski, A., & Kumar, S. (2013). MEGA6: Molecular evolutionary genetics analysis version 6.0. Mol. Biol. Evol., 30(12), 2725–2729.

    CAS  Google Scholar 

  • Torres, M. A. O., Mondêgo-Oliveira, R., De-Sá, J. C., Rocha, A. L., & Abreu-Silva, A. L. (2017). One plant, many uses: A review of the pharmacological applications of morinda citrifolia. Phytotherapy Research, 31(2), 971–979.

    Article  Google Scholar 

  • Trapnell, C., Pachter, L., & Salzberg, S. L. (2009). TopHat: Discovering splice junctions with RNA-Seq. Bioinformatics, 25(9), 1105–1111.

    Article  CAS  Google Scholar 

  • Vozárová, Z., Glasa, M., & Šubr, Z. W. (2017). Tracking the potyviral P1 protein in Nicotiana benthamiana plants during plum pox virus infection. Acta Virologica, 61(4), 492–494.

    Article  Google Scholar 

  • Wang, Y. F., Liu, Y. X., & Cai, Z. Y. (2015). Biological characteristics of noni anthracnose. Guangdong Agricultural Sciences, 42(7), 60–63.

    CAS  Google Scholar 

  • Wylie, S. J., Adams, M., Chalam, C., Kreuze, J., López-Moya, J. J., Ohshima, K., Praveen, S., Rabenstein, F., Stenger, D., Wang, A., & Zerbini, F. M. (2017). ICTV report consortium, ICTV virus taxonomy profile: Potyviridae. The Journal of General Virology, 98, 352–354.

    Article  CAS  Google Scholar 

  • Yang, K., Shen, W. T., Li, Y., Li, Z. P., Miao, W. G., Wang, A. M., & Cui, H. G. (2019). Areca palm necrotic ringspot virus, classified within a recently proposed genus Arepavirus of the family Potyviridae, is associated with necrotic ringspot disease in areca palm. Phytopathology, 109(5), 887–894.

    Article  Google Scholar 

  • Yu, N. T., Xie, H. M., Zhang, Y. L., Wang, J. H., Xiong, Z., & Liu, Z. X. (2019). Independent modulation of individual genomic component transcription and a cis-acting element related to high transcriptional activity in a multipartite DNA virus. BMC Genomics, 20(1), 573.

    Article  Google Scholar 

Download references

Funding

This study was funded by the Major Science and Technology Program of Hainan Province (grant number ZDKJ2017003), Young Elite Scientists Sponsorship Program by CSTC (No. CSTC-QN201704) and Guangdong Key Laboratory of Tropical and Subtropical Fruit Tree Research, Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences (No. 2017B030314113).

Author information

Authors and Affiliations

  1. Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture and Rural Affairs, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, China

    Nai-Tong Yu & Zhi-Xin Liu

  2. Yunnan Institute of Tropical Crops, Jinhong, 666100, China

    Zhi-Ying Cai & Yan Yang

  3. School of Plant Sciences and BIO5 Institute, University of Arizona, Tucson, AZ, 85721, USA

    Zhongguo Xiong

Authors
  1. Nai-Tong Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Zhi-Ying Cai
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Zhongguo Xiong
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yan Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Zhi-Xin Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Nai-Tong Yu, Yan Yang or Zhi-Xin Liu.

Ethics declarations

Conflict of interest

The authors declare no conflict of interest.

Ethical approval

This manuscript did not involve any human participants, and/ or animals.

Electronic supplementary material

ESM 1

(DOCX 14 kb)

ESM 2

(DOCX 15 kb)

ESM 3

(DOCX 15 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Yu, NT., Cai, ZY., Xiong, Z. et al. Complete genomic sequence of Noni mosaic virus (NoMV) associated with a mosaic disease in Morinda citrifolia L.. Eur J Plant Pathol 156, 1005–1014 (2020). https://doi.org/10.1007/s10658-020-01948-4

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10658-020-01948-4

Keywords

Search

Navigation