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Histopathology of Phakopsora euvitis on Vitis vinifera

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Abstract

Rust (Phakopsora euvitis) is an important fungal disease in grapevines grown in tropical and subtropical regions. Epidemiological works have been conducted on plant disease resistance, however, little is known about defense mechanisms of resistance to the disease. Leaves of Vitis vinifera cv. Moscato Giallo were inoculated with P. euvitis and lesions were formed with pustules surrounded by water-soaked halo 17 days after inoculation. Foliar tissue of injured and sound material was fixed and submitted to histological techniques. Emergence of pustules from stomata was observed. In water-soaked halo, hyphae of fungus were not observed. In this region, foliar blade presented mesophyll modified by cell hypertrophy with reduction of intercellular spaces and accumulation of pectic compounds. Hypertrophied cells showed parietal thickenings in the cellulose and pectin layers. In the areas delimited by water-soaked halo in the pustule region, the fungus grew vigorously in intercellular spaces of chlorophyll parenchyma; however, vascular bundles also restricted the advance of fungus where sheath cells present parietal pectic thickenings. Therefore, although Vitis vinifera cv. Moscato showed rust symptoms on leaves, pathogen colonization was limited by the formation of water-soaked haloes and vascular bundles, which resulted in minor injuries along the foliar limbo.

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Acknowledgements

The authors gratefully acknowledge the financial support of the São Paulo Research Foundation (FAPESP Proc: 2013/24003-9). We thank the Center of Support in Electron Microscopy Applied to Agriculture, ESALQ, USP, at the laboratory of Plant Anatomy of ESALQ (LanVeg) for the use of equipment. We also thank Msc. Marli Kasue Missake Soares for helping in material preparation for the anatomical analysis. BLN thanks Dr. Antonio F. Nogueira Jr. for his comments to the manuscript.

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  1. Barbara Ludwig Navarro

    Present address: Department of Crop Science, Division Plant Pathology and Crop Protection, George-August-University Göttingen, Grisebachstraße 6, 37077, Göttingen, Germany

Authors and Affiliations

  1. Department of Plant Pathology and Nematology, University of São Paulo, São Paulo, Brazil

    Barbara Ludwig Navarro

  2. Laboratory of Nuclear Instrumentation, Center of Nuclear Energy to Agriculture, University of São Paulo, São Paulo, Brazil

    João Paulo Rodrigues Marques

  3. Department of Biological Science, University of São Paulo, São Paulo, Brazil

    Beatriz Appezzato-da- Glória & Marcel Bellato Spósito

  4. Department of Crop Science, University of São Paulo, São Paulo, Brazil

    Marcel Bellato Spósito

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  1. Barbara Ludwig Navarro
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  2. João Paulo Rodrigues Marques
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  3. Beatriz Appezzato-da- Glória
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  4. Marcel Bellato Spósito
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Correspondence to Marcel Bellato Spósito.

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Navarro, B.L., Marques, J.P.R., Appezzato-da- Glória, B. et al. Histopathology of Phakopsora euvitis on Vitis vinifera. Eur J Plant Pathol 154, 1185–1193 (2019). https://doi.org/10.1007/s10658-019-01719-w

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