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Genetic diversity and pathogenicity dynamics of Magnaporthe oryzae in the Wuling Mountain area of China

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Abstract

Rice blast, caused by Magnaporthe oryzae (M. oryzae), is one of the most destructive diseases in cultivated rice. The Wuling Mountain Area is marked as the key area for the national prevention and control of rice blast disease in China and is the ideal area for M. oryzae research according to its unique natural condition. In this study, a simple, low-cost and effective marker system including RAPD, REMAP, rep-PCR and Avr genes was developed for genetic diversity analysis of 108 M. oryzae isolates collected from the Wuling Mountain Area during 2012–2014. The results showed significant changes in the genetic structures of M. oryzae populations over the 3 years. However, there was no remarkably simple relationship between the genetic lineages and pathotypes (physiological races) of M. oryzae. The main mechanisms of M. oryzae to overcome host genes in the natural conditions of the Wuling Mountain Area might be point mutations or small deletions of gene sequences.

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Acknowledgements

We gratefully acknowledge Dr. Xiaoyuan Zhu (the Plant Protection Research Institute of the Guangdong Academy of Agricultural Sciences, China) for the help of disease evaluation.

Funding

We are grateful for grants from the Science Funds for the Creative Research Groups of Hubei Province, China (No. 2015CFA015) and the Fundamental Research Funds for the Central Universities, South-Central University for Nationalities, China (No. CZP17029 & CZP17089).

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Author notes

  1. Xin Xu and Wu Yang contributed equally to this work.

Authors and Affiliations

  1. Hubei Provincial Key Laboratory for Protection and Application of Special Plant Germplasm in Wuling Area of China, Key Laboratory of State Ethnic Affairs Commission for Biological Technology, College of Life Sciences, South-Central University for Nationalities, Wuhan, 430074, China

    Xin Xu, Wu Yang, Ke Tian, Jie Zheng, Xinqiong Liu, Kai Li, Wei Lu, Yanping Tan, Yonghua Qin & Chuntai Wang

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  1. Xin Xu
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Correspondence to Chuntai Wang.

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Figure S1A

Amplification frequencies of Avr genes in 108 M. oryzae isolates collected in Wuling Mountain Area from 2012 to 2014. Frequency of the number of Avr genes carried (JPG 119 kb)

Figure S1A

Amplification frequencies of Avr genes in 108 M. oryzae isolates collected in Wuling Mountain Area from 2012 to 2014. Frequency of a single Avr gene (JPG 52.1 kb)

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Xu, X., Yang, W., Tian, K. et al. Genetic diversity and pathogenicity dynamics of Magnaporthe oryzae in the Wuling Mountain area of China. Eur J Plant Pathol 153, 731–742 (2019). https://doi.org/10.1007/s10658-018-1587-4

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