Abstract
Thiabendazole and thiophanate-methyl (methyl benzimidazole carbamate group — MBC) are fungicides commonly used in mango orchards in the Brazilian Northeast, although they are not registered to control dieback and stem-end rot. However, populations of Botryosphaeriaceae are exposed to these fungicides, and resistance may evolve. Therefore, the sensitivity of 154 Botryosphaeriaceae isolates was evaluated by analyzing the effective concentration required to inhibit 50% of mycelial growth (EC50). Ten isolates with the lowest (sensitive—S) and highest (less sensitive—LS) EC50 values for thiabendazole and thiophanate-methyl were evaluated for the stability of the sensitivity, the molecular mechanisms behind low sensitivity, the effectiveness of MBCs to control disease in mango fruits and the components of fitness. Isolates exhibited EC50 values ranging from 0.03 to 6.86 μg ml−1 and from 0.0001 to 10.70 μg ml−1 for thiabendazole and thiophanate-methyl, respectively. A significant and positive correlation (P ≤ 0.01; r = 0.82) was detected between the sensitivity of the isolates to both fungicides. The LS isolates were able to maintain this characteristic after 10 generations and did not have reduced fitness compared with the S isolates. Nevertheless, the most common mutation associated with a low sensitivity to MBCs (at codon 198 of the β-tubulin gene) was not found. Both fungicides were able to control the infection caused by S and LS isolates similarly. Thus, thiabendazole and thiophanate-methyl can be used to control dieback and stem-end rot in mango orchards in the Brazilian Northeast.
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Acknowledgements
The first author thanks the Conselho Nacional de Desenvolvimento Científico e Tecnológico – CNPq for a master scholarship and S. J. Michereff and M. P. S. Câmara are thankful for their research fellowship.
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dos Santos, K.M., Tsuji, S.S., Câmara, M.P.S. et al. Sensitivity to methyl benzimidazole carbamate fungicides of Botryosphaeriaceae species from mango orchards in the Northeast of Brazil. Eur J Plant Pathol 153, 209–222 (2019). https://doi.org/10.1007/s10658-018-1556-y
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DOI: https://doi.org/10.1007/s10658-018-1556-y