Outcome of sexual reproduction in the Phytophthora infestans population in Estonian potato fields
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In this study, the Estonian population of Phytophthora infestans was characterized with mating type, sensitivity to metalaxyl, virulence on 11 potato R-gene differentials and 12 SSR markers to show the outcome of potential sexual reproduction in the population. During the three years 2010–2012, 141 P. infestans isolates, collected from 23 potato fields, showed quite a high and stable frequency of the A2 mating type, 48% of the total population. In 87% of all sampled potato fields, both mating types were recorded, suggesting continuous sexual reproduction of P. infestans and possible oospore production. Metalaxyl-sensitive isolates prevailed in all three years (68 out of 99 isolates). Amongst the 95 isolates tested, 51 virulence races were found. The race structure was diverse, and most pathotypes were unique, appearing only once; the two most common pathotypes, 22.214.171.124.126.96.36.199 and 188.8.131.52.7.10.11, comprised 35% of the population. The P. infestans population was genetically highly diverse and most of the multilocus genotypes (MLGs) appeared only once. Furthermore, all of the MLGs appeared in only one of the three sampling years. Our results confirm that the high diversity in the Estonian P. infestans population is most likely the result of frequent sexual reproduction, which benefits the survival, adaptability and diversity of the pathogen in the climate of North-Eastern Europe.
KeywordsSSR markers Genetic diversity Mating type Virulence Metalaxyl resistance Late blight
Alice Aav, Gerit Dreyersdorff, Kätlin Jõgi, Liis Laane, Helina Nassar, Terje Tähtjärv and Grete Zahkna are thanked for technical support. We are grateful to Asko Hannukkala from the Natural Resources Institute Finland (Luke) for supplying tester isolates for mating type determination. Many thanks too to Dr. Eva Randall at the James Hutton Institute for technical assistance.
This study was supported by Estonian Foundation grant no 9432, Institutional research funding IUT36–2 of the Estonian Ministry of Education and Research, projects RESIST 3.2.0701.11–0003 and IPMBlight 2.0 8T150054PKTK. The Scottish Government is acknowledged for funding at the James Hutton Institute. The study visit to The James Hutton Institute was supported by the European Social Fund’s Doctoral Studies and Internationalisation Programme DoRa, which is carried out by Foundation Archimedes.
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Conflict of interest
The authors declare that they have no conflict of interest.
- Balloux, F., Lehmann, L., & De Meeus, T. (2003). The population genetics of clonal and partially clonal diploids. Genetics, 164, 1635–1644.Google Scholar
- Chowdappa, P., Nirmal Kumar, B. J., Madhura, S., Mohan Kumar, S. P., Myers, K. L., Fry, W. E., & Cooke, D. E. L. (2015). Severe outbreaks of late blight on potato and tomato in South India caused by recent changes in the Phytophthora infestans population. Plant Pathology, 64, 191–199.CrossRefGoogle Scholar
- Cooke, D. E. L., Cano, L. M., Raffaele, S., Bain, R. A., Cooke, L. R., Etherington, G. J., Deahl, K. L., Farrer, R. A., Gilroy, E. M., Goss, E. M., Grünwald, N. J., Hein, I., MacLean, D., McNicol, J. W., Randall, E., Oliva, R. F., Pel, M. A., Shaw, D. S., Squires, J. N., Taylor, M. C., Vleeshouwers, V. G. A. A., Birch, P. R. J., Lees, A. K., & Kamoun, S. (2012). Genome analyses of an aggressive and invasive lineage of the Irish potato famine pathogen. PLoS Pathogens, 8, e1002940.CrossRefPubMedPubMedCentralGoogle Scholar
- Cooke, L. R., Schepers, H. T. A. M., Hermansen, A., Bain, R. A., Bradshaw, N. J., Ritchie, F., Shaw, D. S., Evenhuis, A., Kessel, G. J. T., Wander, J. G. N., Andersson, B., Hansen, J. G., Hannukkala, A., Nærstad, R., & Nielsen, B. J. (2011). Epidemiology and integrated control of potato late blight in Europe. Potato Research, 54, 183–222.CrossRefGoogle Scholar
- Estonian Weather Service (2016). Climate normals. http://www.ilmateenistus.ee/kliima/kliimanormid/ohutemperatuur/?lang=en. Accessed 16 July 2016.
- EuroBlight (2017). A potato late blight network for Europe. euroblight.net. Accessed 1 March 2017.
- Flier, W. G., Kroon, L. P. N. M., Hermansen, A., van Raaij, H. M. G., Speiser, B., Tamm, L., Fuchs, J. G., Lambion, J., Razzaghian, J., Andrivon, D., Wilcockson, S., & Leifert, C. (2007). Genetic structure and pathogenicity of populations of Phytophthora infestans from organic potato crops in France, Norway, Switzerland and the United Kingdom. Plant Pathology, 56, 562–572.CrossRefGoogle Scholar
- Fry, W. E., McGrath, M. T., Seaman, A., Zitter, T. A., McLeod, A., Danies, G., Small, I. M., Myers, K., Everts, K., Gevens, A. J., Gugino, B. K., Johnson, S. B., Judelson, H., Ristaino, J., Roberts, P., Secor, G., Seebold Jr., K., Snover-Clift, K., Wyenandt, A., Grünwald, N. J., & Smart, C. D. (2013). The 2009 late blight pandemic in the eastern United States – causes and results. Plant Disease, 97, 296–306.CrossRefGoogle Scholar
- Hannukkala, A. O. (2012). History and consequences of migrations, changes in epidemiology and population structure of potato late blight, Phytophthora infestans, in Finland from 1845 to 2011. Doctoral Dissertation. MTT Science 18. MTT Agrifood Research Finland, Jokioinen, 136 p. Available at http://www.mtt.fi/mtttiede/pdf/mtttiede18.pdf.
- Li, Y., van der Lee, T. A. J., Evenhuis, A., van den Bosch, G. B. M., van Bekkum, P. J., Förch, M. G., et al. (2012). Population dynamics of Phytophthora infestans in the Netherlands reveals expansion and spread of dominant clonal lineages and virulence in sexual offspring. G3. Genes Genomes Genetics, 2, 1529–1540.PubMedGoogle Scholar
- Li, Y., van der Lee, T., Zhu, J. H., Jin, G. H., Lan, C. Z., Zhu, S. X., Zhang, R. F., Liu, B. W., Zhao, Z. J., Kessel, G., Huang, S. W., & Jacobsen, E. (2013b). Population structure of Phytophthora infestans in China – Geographic clusters and presence of the EU genotype Blue_13. Plant Pathology, 62, 932–942.CrossRefGoogle Scholar
- Mayton, H., Smart, C. D., Moravec, B. C., Mizubuti, E. S. G., Muldoon, A. E., & Fry, W. E. (2000). Oospore survival and pathogenicity of single oospore recombinant progeny from 23 a cross involving US-17 and US-8 genotypes of Phytophthora infestans. Plant Disease, 84, 1190–1196.CrossRefGoogle Scholar
- Montarry, J., Andrivon, D., Glais, I., Corbiere, R., Mialdea, G., & Delmotte, F. (2010). Microsatellite markers reveal two admixed genetic groups and an ongoing displacement within the French population of the invasive plant pathogen Phytophthora infestans. Molecular Ecology, 19, 1965–1977.CrossRefPubMedGoogle Scholar
- Pobedinskaya, M. A., Elansky, S. N., Statsyuk, N. V., & Plyakhnevich, M. P. (2011). Fungicide resistance of Russian Phytophthora infestans strains. PPO – Special Report, 15, 243–248.Google Scholar
- Runno-Paurson, E., Fry, W. E., Remmel, T., Mänd, M., & Myers, K. L. (2010). Phenotypic and genotypic characterisation of Estonian isolates of Phytophthora infestans in 2004-2007. Journal of Plant Pathology, 92, 375–384.Google Scholar
- Runno-Paurson, E., Hannukkala, A., Williams, I., Koppel, M., & Mänd, M. (2012). The structure of mating type, virulence, metalaxyl resistance of Phytophthora infestans in a long-term phenotypic study in distinct location in eastern Estonia. Journal of Plant Diseases and Protection, 119, 45–52.CrossRefGoogle Scholar
- Runno-Paurson, E., Williams, I. H., Metspalu, L., Kaart, T., & Mänd, M. (2013a). Current potato varieties are too susceptible to late blight to be grown without chemical control under north-east European conditions. Acta Agriculturae Scandinavica, Section B – Soil & Plant Science, 63, 80–88.Google Scholar
- Runno-Paurson, E., Hannukkala, A., Kotkas, K., Koppel, M., Williams, I. H., & Mänd, M. (2014). Population changes and phenotypic diversity of Phytophthora infestans isolates from Estonia and Finland. Journal of Plant Pathology, 96, 85–95.Google Scholar
- Śliwka, J., Sobkowiak, S., Lebecka, R., Avendańo Córcoles, J., & Zimnoch-Guzowska, E. (2006). Mating type, virulence, aggressiveness and metalaxyl resistance of isolates of Phytophthora infestans in Poland. Potato Research, 49, 155–166.Google Scholar
- Statsyuk, N. V., Kozlovskaya, I. N., Koslovsky, B. E., Ulanova, T. I., Morozova, E. V., & Kuznetsova, M. (2013). Changes in phenotypic characteristics of the Moscow Phytophthora infestans population in the period of 2000–2011. Proceedings of the 4th International Symposium “Agrosym 2013” (pp. 607–613). Istočno Sarajevo: Jahorina.Google Scholar
- Statsyuk, N. V., Semina, Y. V., Perez, F. G. M., Larsen, M. M., Kuznetsova, M. A., Kozlovskaya, I. N., et al. (2014). Characterization of Russian Phytophthora infestans populations: DNA fingerprinting and SSR analysis. PPO – Special report, 16, 255–266.Google Scholar
- USAblight (2017). A national project on tomato & potato late blight in the United States. https://usablight.org/. Accessed 1 March 2017.
- Vorobyeva, Y. V., Gridnev, V. V., Bashaeva, E. G., Pospelova, L. A., Kvasnyuk, N. Y., Kuznetsova, L. N., et al. (1991). On the occurrence of the A2 mating type isolates of Phytophthora infestans (Mont.) de Bary in the USSR. Mikologija i fitopatologija, 62–67.Google Scholar