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Comparative colonisation by virulent versus avirulent Pyricularia oryzae on wild Oryza australiensis

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Abstract

Pyricularia oryzae (rice blast) conidial development at pre-penetration stage determines success or otherwise of infection inside the rice host plants. Studies on conidial germination and growth on the leaf surface in commercial rice (Oryza sativa) report differently, dependent upon host type and level of blast resistance. Although wild rice (O. australiensis) is known to be an alternative host of blast, the interaction between P. oryzae conidia and wild O. australiensis on its leaf surface has not been previously studied. We found significant (P < 0.001) differences in conidial development between two blast isolates with different virulence in terms of conidial germination, germ tube growth and appressoria formation on both wild and cultivated rice. Conidial germination at 6 h post-inoculation (hpi) for the virulent isolate was significantly (P < 0.001) delayed. Germ tubes of the avirulent isolate conidia grew significantly (P < 0.001) faster and with significantly (P < 0.001) longer germ tubes than from virulent conidia. Appressoria development for the virulent isolate was significantly (P < 0.001) faster at its later growth stages of 12 and 18 hpi when approximately 100% of germ tubes formed appressoria. In contrast, formation rate of appressoria for the avirulent isolate was significantly (P < 0.001) slower and only reached 76% of germ tubes forming appressoria. Appressoria formation on O. australiensis was significantly (P < 0.001) greater than the formation on O. sativa for both virulent and avirulent P. oryzae at 12 hpi, a clear indication that host type influences the extent of appressoria formation.

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Acknowledgements

The first author gratefully acknowledges an Australian Government Endeavour Postgraduate Scholarship funding his PhD studies. We are very grateful to the University of Western Australia and the Rural Industries Research and Development Corporation (RIRDC project PRJ-008565 ‘Characterisation of rice blast races present in Australia’) for supporting this research and to Dr. Peta Clode and Ms. Lyn Kirilak, in the Centre for Microscopic, Characterization, and Analysis at the University of Western Australia for help with microscopy studies.

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Authors and Affiliations

  1. UWA School of Agriculture and Environment, Faculty of Science, The University of Western Australia, 35 Stirling Highway, Crawley, WA, 6009, USA

    Dolar Pak, Ming Pei You & Martin J. Barbetti

  2. The UWA Institute of Agriculture, Faculty of Science, The University of Western Australia, 35 Stirling Highway, Crawley, WA, 6009, USA

    Ming Pei You & Martin J. Barbetti

  3. Department of Agriculture and Food Western Australia, Baron-Hay Court, South Perth, WA, 6151, Australia

    Vincent Lanoiselet

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  1. Dolar Pak
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  2. Ming Pei You
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  3. Vincent Lanoiselet
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  4. Martin J. Barbetti
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Correspondence to Martin J. Barbetti.

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This research did not involve any animal and/or human participants. The authors declare that they have no conflict of interests.

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Pak, D., You, M.P., Lanoiselet, V. et al. Comparative colonisation by virulent versus avirulent Pyricularia oryzae on wild Oryza australiensis. Eur J Plant Pathol 151, 927–936 (2018). https://doi.org/10.1007/s10658-018-1428-5

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