Germplasm screening of Musa spp. for resistance to Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4)

  • Cunwu Zuo
  • Guiming Deng
  • Bin Li
  • Heqiang Huo
  • Chunyu Li
  • Chunhua Hu
  • Ruibin Kuang
  • Qiaosong Yang
  • Tao Dong
  • Ou Sheng
  • Ganjun Yi
Article
  • 86 Downloads

Abstract

Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4) has seriously threatened global banana production. Recently, identification of Foc TR4 in the Caribbean and Mozambique has aroused widespread concern because it may be directly related to food security for millions of people in Africa and Latin America. To identify a resistant or highly resistant germplasm, 129 accessions were evaluated for Foc TR4 resistance in greenhouses and 100 of them were also assessed in the field. In total, 10 accessions were highly resistant (HR) to Foc TR4; these include four from the AA group, two from the BB group, one of the East African highland bananas (EAHBs), two plantains, and one from wild relatives of banana. Of these, Pahang (AA), Calcutta 4 (AA), and Musa itinerans exhibited the highest degree of resistance with an index of disease (ID2) less than 10, and Foc TR4 could not infect any of them in the field. Furthermore, 31 accessions from AA, AB, AAB, AAAB, and ABB groups and wild relatives were identified as a resistant (R) germplasms. All tested EAHBs and plantains exhibited resistance or high resistance to Foc TR4 infection. Our results provide valuable genetic resources for banana breeding and for studying the mechanisms underlying Fusarium wilt resistance. Furthermore, evaluation of EAHBs and plantains provided a rational for local banana producers and researchers to grow EAHBS and plantains.

Keywords

Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4) Resistance evaluation Genome type East African highland bananas (EAHBs) Plantains 

Abbreviations

CARBAP

Ctr Africain Rech Bananiers & Plantains

EAHBs

East African Highland Bananas

Foc

Fusarium oxysporum f. sp. cubense

Foc TR4

Foc tropical race 4

Foc STR4

Foc subtropical race 4

HR

Highly resistant

HS

Highly susceptible

I

Immune

ID1

Index of disease

ID2

Incidence of disease

IITA

International institute of tropical agriculture

ITC

International Transit Center

MR

Moderately resistant

PDA

Potato dextrose agar

PDB

Potato dextrose broth

R

Resistant

S

Susceptible

Notes

Acknowledgements

We thank Dr. Zhongjian Chen (Argo-biological Gene Research Center, Guangdong Academy of Agricultural Sciences, Guangzhou, China) to revise and edit the final version of the manuscript.

Compliance with ethical standards

Competing interests

The authors declare that they have no competing interests.

Consent for publication

Not applicable.

Ethics approval and consent to participate

Not applicable.

Supplementary material

10658_2017_1406_Fig2_ESM.gif (603 kb)
Fig. S1

Foc TR4 detection using specific primers. For each sample, different genomic DNA was used as a substrate for PCR amplification. C-: non-infected banana pseudostem; C+: Foc TR4 strain GD-13; 1 to 10: Foc strains isolated from infected banana pseudostem after inoculation. (GIF 603 kb)

10658_2017_1406_MOESM1_ESM.tif (314 kb)
High Resolution Image (TIFF 313 kb)
10658_2017_1406_MOESM2_ESM.docx (26 kb)
Table S1 (DOCX 25 kb)

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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2018

Authors and Affiliations

  • Cunwu Zuo
    • 1
    • 2
  • Guiming Deng
    • 1
    • 3
  • Bin Li
    • 4
  • Heqiang Huo
    • 5
  • Chunyu Li
    • 1
    • 3
  • Chunhua Hu
    • 1
    • 3
  • Ruibin Kuang
    • 1
    • 3
  • Qiaosong Yang
    • 1
    • 3
  • Tao Dong
    • 1
    • 3
  • Ou Sheng
    • 1
    • 3
  • Ganjun Yi
    • 1
    • 3
  1. 1.Institute of Fruit Tree ResearchGuangdong Academy of Agricultural Sciences (IFTR-GDAAS)GuangzhouChina
  2. 2.College of HorticultureGansu Agricultural UniversityLanzhouChina
  3. 3.Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of AgricultureGuangzhouChina
  4. 4.Collage of Life ScienceSouth China Agricultural UniversityGuangzhouChina
  5. 5.Mid-Research and Education CenterUniversity of FloridaApopkaUSA

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