European Journal of Plant Pathology

, Volume 148, Issue 3, pp 749–754 | Cite as

Fusarium verticillioides inoculum potential influences soybean seed quality



F. verticillioides (FVE) is an important Fusarium species that has been recovered from soybean seeds. In other crops, such as maize, it has the potential to decrease seed germination and vigor. The objective of this study was to evaluate the influence of seed inoculum potential (amount of inoculum present in seeds) of FVE on soybean seed quality, as measured by germination of artificially inoculated seeds. Seeds were inoculated with 2.5 × 106 FVE conidia/ml for one minute as the low inoculum potential seed treatment. For the high inoculum potential seed treatment, inoculated seeds were inoculated with the same conidia suspension, but osmoconditioned on potato dextrose agar +8% mannitol for 48 h. Two soybean genotypes, ‘AG3039’ and ‘KSU3406’ were tested. Analysis of seeds inoculated with the low inoculum potential treatment showed that none of the FVE isolates tested and only the positive controls were able to significantly reduce soybean seed germination (P < 0.001) when compared with the mock-inoculated control for both genotypes tested. Under the high inoculum potential treatment, all three FVE isolates were able to decrease seed germination when compared with the mock-inoculated control treatment for both genotypes. This study suggests that F. verticillioides has the potential to reduce soybean seed quality, depending on the amount of inoculum present in seeds (inoculum potential), which affects pathogenicity and negatively influences soybean seed germination as well as the establishment of a uniform and healthy stand in the field.


Seedborne fungi Fusarium verticillioides Soybean seed quality Inoculum potential 



This publication is Contribution No. 17-127-J from the Kansas Agricultural Experiment Station, Manhattan. Funding for this project was provided in part by the United Soybean Board (Project 1520-532-5667).

Supplementary material

10658_2016_1127_Fig4_ESM.gif (273 kb)
Supplementary Figure 1

Soybean seed inoculation methods. (a) Low inoculum potential treatment: Soybean seeds were imbibed in 25 ml of Fusarium inoculum suspension (2.5 × 105 conidia ml−1) for 1 min (a.1); Seeds were placed on germination paper after inoculation (a.2); After 7 days of incubation at 25 °C and 88% R.H., germination (%), and DSI were calculated based on symptoms observed (a.3). High inoculum potential treatment: Soybean seeds imbibed in 25 ml of Fusarium inoculum suspension for 1 min (b.1); Inoculated seeds were placed on PDA, osmotically modified with mannitol (PDA + M) for 48 h (b.2); After incubation of the seeds for 48 h at 25 °C, artificially inoculated seeds were dried for 2 days at room temperature (b.3); One hundred inoculated seeds were placed on germination paper after the inoculation procedure (b.4); After 7 days of incubation at 25 °C and 88% R.H., germination (%), and DSI were calculated (b.5). (GIF 273 kb)

10658_2016_1127_MOESM1_ESM.tif (19.6 mb)
High resolution image (TIFF 20057 kb)


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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2016

Authors and Affiliations

  1. 1.Department of Plant PathologyKansas State UniversityManhattanUSA

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