Evaluation of Gram-positive rhizosphere and endophytic bacteria for biological control of fungal rice (Oryzia sativa L.) pathogens
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Gram-positive bacteria isolated from the rhizosphere and inside the roots of rice were characterized for plant growth promoting (PGP) traits and antifungal activity against some rice plant pathogenic fungi of rice. The results showed the endophytic and rhizosphere isolates had different PGP traits and antifungal activity. Only one rhizosphere isolate and one endophytic isolate showed highly inhibitory effects against the mycelial growth of all fungal rice pathogens tested in this study. The best bacterial isolates, based on multiple PGP traits and inhibitory effects against the mycelial growth of all fungal rice pathogens, were identified. Based on biochemical tests and by comparison of 16S rDNA sequences, the endophytic isolate REN3 and the rhizosphere isolate REN4 were closely related to Bacillus cereus and Bacillus mojavensis respectively. The broad-spectrum antifungal strains, the REN3 and REN4 isolates analyzed here, exert multiple PGP and antagonistic activity and represent an excellent option to be used as either potent bio-promoting or bio-control agents in rice under in vitro conditions. This application may help to minimize dependence on pesticides, which have adverse effects on the environment, finally leading to have sustainable environments. In conclusion, the results of antifungal activity showed rice harbors bacteria with a good potential in biocontrol of rice fungal pathogens.
KeywordsRhizosphere and endophytic bacteria Rice PGPR Multiple PGP traits Antifungal activity
We thank the Center of Excellence for Soil Quality Improvement for Balanced Plant Nutrition, Department of Soil Science, Faculty of Agricultural Engineering and Technology, University of Tehran, for funding a part of this research.
- Etesami, H., & Alikhani, H. A. (2016b). Suppression of the fungal pathogen Magnaporthe grisea by Stenotrophomonas maltophilia, a seed-borne rice (Oryza sativa L.) endophytic bacterium. Archives of Agronomy and Soil Science (just-accepted).Google Scholar
- Etesami, H., Hosseini, H. M., & Alikhani, H. A. (2014a). Bacterial biosynthesis of 1-aminocyclopropane-1-caboxylate (ACC) deaminase, a useful trait to elongation and endophytic colonization of the roots of rice under constant flooded conditions. Physiology and Molecular Biology of Plants, 20(4), 425–434.CrossRefPubMedPubMedCentralGoogle Scholar
- Etesami, H., Hosseini, H. M., Alikhani, H. A., & Mohammadi, L. (2014b). Bacterial biosynthesis of 1-aminocyclopropane-1-carboxylate (ACC) deaminase and indole-3-acetic acid (IAA) as endophytic preferential selection traits by rice plant seedlings. Journal of Plant Growth Regulation, 33(3), 654–670.CrossRefGoogle Scholar
- Kobayashi, D., & Palumbo, J. (2000). Bacterial endophytes and their effects on plants and uses in agriculture. Microbial endophytes, 199–233.Google Scholar
- Kuarabachew, H., Assefa, F., & Hiskias, Y. (2007). Evaluation of Ethiopian isolates of Pseudomonas fluorescens as biocontrol agent against potato bacterial wilt caused by Ralstonia (Pseudomonas) solanacearum. Acta Agriculturae Solvenica, 90(2), 125–135.Google Scholar
- Li, X., Hu, B., Xu, Z., & Mew, T. (2003). Threshold population sizes of Bacillus Subtilis B5423-R to suppress the occurrence of rice sheath blight. China Journal Rice Science, 17(4), 360–364.Google Scholar
- Maheshwari, D., Aeron, A., Dubey, R., Agarwal, M., Dheeman, S., & Shukla, S. (2014). Multifaceted beneficial associations with pseudomonas and rhizobia on growth promotion of Mucuna pruriens L. Journal Pure Applied Microbiology, 8(6), 4657–4667.Google Scholar
- Mendes, R., Pizzirani-Kleiner, A. A., Araujo, W. L., & Raaijmakers, J. M. (2007). Diversity of cultivated endophytic bacteria from sugarcane: genetic and biochemical characterization of Burkholderia cepacia complex isolates. Applied and Environmental Microbiology, 73(22), 7259–7267.CrossRefPubMedPubMedCentralGoogle Scholar
- Pikovskaya, R. I. (1948). Mobilization of phosphorus in soil connection with the vital activity of some microbial species. Microbiologiya, 17, 362–370.Google Scholar
- Zinniel, D. K., Lambrecht, P., Harris, N. B., Feng, Z., Kuczmarski, D., Higley, P., et al. (2002). Isolation and characterization of endophytic colonizing bacteria from agronomic crops and prairie plants. Applied and Environmental Microbiology, 68(5), 2198–2208.CrossRefPubMedPubMedCentralGoogle Scholar