European Journal of Plant Pathology

, Volume 146, Issue 1, pp 219–224 | Cite as

Colletotrichum gloeosporioides associated with anthracnose symptoms on citrus, a new report for Tunisia

  • Azza Rhaiem
  • Paul W. J. Taylor


Colletotrichum gloeosporioides causing anthracnose disease of citrus is reported for the first time in Tunisia. Since early 2009, wither-tip symptoms of twigs, resembling disease caused by anthracnose were observed on cultivated citrus trees in Tunisia. Cultural and morphological features of the identified causal agent were consistent and resembled those described for C. gloeosporioides. These results were confirmed by sequencing of the internal spacer rDNA (ITS), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), glutamine synthetase (GS) and the ApMat locus regions and by multilocus phylogenetic analyses. Pathogenicity tests revealed that C. gloeosporioides isolated from withered twigs caused typical anthracnose symptoms on citrus fruits and leaves.

Key words

Colletotrichum gloeosporioides Citrus Tunisia Morphology Phylogeny 



Part of this work was financially supported by the Crawford Fund Australia. We thank Pf. Mohamed Cherif (National Agronomic Institute of Tunisia/Citrus Technical Center, Tunisia) for his kind support.

Supplementary material

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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2016

Authors and Affiliations

  1. 1.National Gene Bank (Banque Nationale de Gènes)Boulevard du leader Yassar ArafatTunisTunisia
  2. 2.Faculty of Veterinary and Agricultural SciencesThe University of MelbourneParkvilleAustralia

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