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Pseudomonas syringae pv. Actinidiae isolated from non-kiwifruit plant species in China

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Abstract

Kiwifruit bacterial canker (KBC), incited by Pseudomonas syringae pv. actinidiae (Psa), may cause severe economic losses to kiwifruit (Actinidia deliciosa and A. chinensis). Many studies have been conducted to clarify relationships between this pathogen and Actinidia species. Conversely, little is known about the chance to colonize alternate hosts and the implications of this possibility on disease spread. In this study, 11 bacterial isolates have been isolated from three wild plant species, Setaria viridis (L.) Beauv., Alternanthera philoxeroides (Mart.) Griseb. and Paulownia tomentosa (Thunb.) Steud., found under or nearby kiwifruit plants affected by bacterial canker. All were identified as Psa based on biochemical tests, specific end-point PCR assays, sequence analysis of single effector genes and MLST. The strains tested positive for pathogenicity, both on wild hosts and on kiwifruit plants. To our knowledge, this is the first report on Psa isolated from non-kiwifruit plants. The proof that Psa can migrate to non-kiwifruit plants raises new issues about the means by which the disease can spread at local and international scales, and the measures to be taken to prevent and control effectively KBC disease.

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Acknowledgments

This project was supported by the Foundation of the National Natural Science Foundation of China (31401831), the Anhui Provincial Natural Science Foundation (1408085QC62) and Discipline Capacity Promotion at Anhui Agricultural University, China (XKTS 2013010). The authors thank Mr. Yang Bing (Tangsheng Kiwifruit Company Ltd., Jinzhai County, Anhui, China) and Mr. Zhang Chao (EF, AHAU, Hefei, China) for their helpful assistance in field sampling.

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Authors and Affiliations

  1. The Key Lab of Pomology, School of Horticulture, Anhui Agricultural University, Hefei, Anhui Province, Peoples Republic of China

    Pu Liu, Shizhou Xue, Rong He, Jiayong Hu, Xiaojie Wang, Bing Jia & Liwu Zhu

  2. Department of Science and Technologies for Agriculture, Forestry, Nature and Energy (DAFNE), University of Tuscia, Viterbo, Italy

    Lorenzo Gallipoli, Angelo Mazzaglia & Giorgio M. Balestra

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  1. Pu Liu
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  2. Shizhou Xue
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  3. Rong He
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  4. Jiayong Hu
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  10. Liwu Zhu
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Correspondence to Liwu Zhu.

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Pu Liu and Shizhou Xue contributed equally to this work.

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Liu, P., Xue, S., He, R. et al. Pseudomonas syringae pv. Actinidiae isolated from non-kiwifruit plant species in China. Eur J Plant Pathol 145, 743–754 (2016). https://doi.org/10.1007/s10658-016-0863-4

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