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European Journal of Plant Pathology

, Volume 145, Issue 4, pp 743–754 | Cite as

Pseudomonas syringae pv. Actinidiae isolated from non-kiwifruit plant species in China

  • Pu Liu
  • Shizhou Xue
  • Rong He
  • Jiayong Hu
  • Xiaojie Wang
  • Bing Jia
  • Lorenzo Gallipoli
  • Angelo Mazzaglia
  • Giorgio M. Balestra
  • Liwu Zhu
Article
  • 694 Downloads

Abstract

Kiwifruit bacterial canker (KBC), incited by Pseudomonas syringae pv. actinidiae (Psa), may cause severe economic losses to kiwifruit (Actinidia deliciosa and A. chinensis). Many studies have been conducted to clarify relationships between this pathogen and Actinidia species. Conversely, little is known about the chance to colonize alternate hosts and the implications of this possibility on disease spread. In this study, 11 bacterial isolates have been isolated from three wild plant species, Setaria viridis (L.) Beauv., Alternanthera philoxeroides (Mart.) Griseb. and Paulownia tomentosa (Thunb.) Steud., found under or nearby kiwifruit plants affected by bacterial canker. All were identified as Psa based on biochemical tests, specific end-point PCR assays, sequence analysis of single effector genes and MLST. The strains tested positive for pathogenicity, both on wild hosts and on kiwifruit plants. To our knowledge, this is the first report on Psa isolated from non-kiwifruit plants. The proof that Psa can migrate to non-kiwifruit plants raises new issues about the means by which the disease can spread at local and international scales, and the measures to be taken to prevent and control effectively KBC disease.

Keywords

Actinidia spp. Pseudomonas syringae pv. Actinidiae (Psa) Interhost Pathogenicity test Multilocus sequence typing 

Notes

Acknowledgments

This project was supported by the Foundation of the National Natural Science Foundation of China (31401831), the Anhui Provincial Natural Science Foundation (1408085QC62) and Discipline Capacity Promotion at Anhui Agricultural University, China (XKTS 2013010). The authors thank Mr. Yang Bing (Tangsheng Kiwifruit Company Ltd., Jinzhai County, Anhui, China) and Mr. Zhang Chao (EF, AHAU, Hefei, China) for their helpful assistance in field sampling.

Supplementary material

10658_2016_863_MOESM1_ESM.docx (2.3 mb)
ESM 1 (DOCX 2331 kb)

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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2016

Authors and Affiliations

  • Pu Liu
    • 1
  • Shizhou Xue
    • 1
  • Rong He
    • 1
  • Jiayong Hu
    • 1
  • Xiaojie Wang
    • 1
  • Bing Jia
    • 1
  • Lorenzo Gallipoli
    • 2
  • Angelo Mazzaglia
    • 2
  • Giorgio M. Balestra
    • 2
  • Liwu Zhu
    • 1
  1. 1.The Key Lab of Pomology, School of HorticultureAnhui Agricultural UniversityHefeiPeoples Republic of China
  2. 2.Department of Science and Technologies for Agriculture, Forestry, Nature and Energy (DAFNE)University of TusciaViterboItaly

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