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European Journal of Plant Pathology

, Volume 139, Issue 4, pp 785–788 | Cite as

Occurrence and molecular variability of Potato spindle tuber viroid and Tomato apical stunt viroid in ornamental plants in Croatia

  • Jasna Milanović
  • Vesna Kajić
  • Snježana Mihaljević
Short Communications

Abstract

Viroids of the genus Pospiviroid are able to induce diseases in a wide range of host plants including important crop species. Although occasional disease outbreaks of Potato spindle tuber viroid (PSTVd) and closely related pospiviroids have been reported in potato and tomato, recent studies found an increase in number of latent infections in ornamental solanaceous species. In order to verify the presence of PSTVd and other pospiviroids in Croatia, a survey was conducted between 2009 and 2012. A total of 182 samples belonging to five ornamental species and two solanaceous crops were analyzed. Eight plants belonging to two different species (Solanum jasminodes and Lycianthes rantonnetii) were found infected by PSTVd and, in addition, one S. jasminoides plant infected by Tomato apical stunt viroid (TASVd). Viroid infection was confirmed by mechanical inoculation on tomato plants to observe symptom expression. Molecular characterization of the isolates was done and complete viroid sequences were submitted to the GenBank. This is the first evidence of the presence of PSTVd and TASVd and their variability in Croatia.

Keywords

Potato spindle tuber viroid Tomato apical stunt viroid Solanaceous ornamentals Sequences 

Notes

Acknowledgements

This research was funded by the Croatian Ministry of Agriculture.

References

  1. Altschul, S. F., Madden, T. L., Schäffer, A. A., Zhang, J., Zhang, Z., Miller, W., et al. (1997). Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Research, 25, 3389–3402.PubMedCentralPubMedCrossRefGoogle Scholar
  2. Corpet, F. (1988). Multiple sequence alignment with hierarchical clustering. Nucleic Acids Research, 16, 10881–10890.PubMedCentralPubMedCrossRefGoogle Scholar
  3. Costantini, M., Luigi, M., Luison, D., Mangiaracina, P., Albanese, G., Tomassoli, L., et al. (2011). Diagnosis, characterization and distribution of pospiviroids in ornamental plants in Italy. Journal of Plant Pathology, 93, S4.14.Google Scholar
  4. Di Serio, F. (2007). Identification and characterization of Potato spindle tuber viroid infecting Solanum jasminodes and S. rantonnetii in Italy. Journal of Plant Pathology, 89, 297–300.Google Scholar
  5. Ding, B., & Itaya, A. (2007). Viroid: A useful model for studying the basic principles of infection and RNA biology. Molecular Plant-Microbe Interactions, 20, 7–20.PubMedCrossRefGoogle Scholar
  6. EPPO (2006a). Further finding of Potato spindle tuber viroid on ornamental Solanaceae in the Netherlands. OEPP/EPPO Reporting Service, 2006/229.Google Scholar
  7. EPPO (2006b). Isolated finding of Potato spindle tuber viroid on Solanum jasminoides in Germany. OEPP/EPPO Reporting Service, 2006/159.Google Scholar
  8. Hadidi, A., Flores, R., Randles, J. W., & Semancik, J. S. (2003). Viroids. Collingwood: CSIRO Publishing.Google Scholar
  9. Luigi, M., Luison, D., Tomassoli, L., & Faggioli, F. (2011). Natural spread and molecular analysis of pospiviroids infecting ornamentals in Italy. Journal of Plant Pathology, 93, 1–5.Google Scholar
  10. Mahfouze, S. A., El-Shamy, M. M. M., & El-Dougdoug, K. A. (2009). Molecular variability of tomato cultivars to Potato spindle tuber viroid infection. Australian Journal of Basic and Applied Sciences, 3, 3321–3329.Google Scholar
  11. Malandraki, I., Papachristopoulou, M., & Vassilakos, N. (2010). First report of Potato spindle tuber viroid (PSTVd) in ornamental plants in Greece. New Disease Reports, 21, 9.CrossRefGoogle Scholar
  12. Matoušek, J., Schubert, J., Beneš, M. J., & Píša, S. (1991). An immunochemical testing of pathophysiological reactions of several PSTVd-infected tomato (Lycopersicon esculentum L.) cultivars. Biologia Plantarum, 33, 358–365.CrossRefGoogle Scholar
  13. Mertelik, J., Kloudova, K., Cervena, G., Necekalova, J., Mikulkova, H., Levkanicova, Z., et al. (2010). First report of Potato spindle tuber viroid (PSTVd) in Brugmansia spp., Solanum jasminoides, Solanum muricatum and Petunia spp. in the Czech Republic. Plant Pathology, 59, 392–392.Google Scholar
  14. Ostroški, L. (2012). Statistical yearbook of the Republic of Croatia 2012. Zagreb: Croatian Bureau of Statistics.Google Scholar
  15. Owens, R. A., Flores, R., Di Serio, F., Li, S. F., Pallás, V., Randles, J. W., et al. (2012). Viroids. In M. Q. K. Andrew, J. A. Michael, B. C. Eric, & J. L. Elliot (Eds.), Virus Taxonomy: Ninth Report of the International Committee on Taxonomy of Viruses (pp. 1221–1234). London: Elsevier Academic Press.Google Scholar
  16. Singh, R. P. (1973). Experimental host range of the potato spindle tuber “virus”. American Potato Journal, 50, 111–123.CrossRefGoogle Scholar
  17. Stark-Lorenzen, P., Guitton, M. C., Werner, R., & Mühlbach, H. P. (1997). Detection and tissue distribution of Potato spindle tuber viroid in infected tomato plants by tissue print hybridization. Archives of Virology, 142, 1289–1296.Google Scholar
  18. Verhoeven, J. Th. J., Jansen, C. C. C., & Roenhorst, J. W. (2008). First report of pospiviroids infecting ornamentals in the Netherlands: Citrus exocortis viroid in Verbena sp., Potato spindle tuber viroid in Brugmansia suaveolens and Solanum jasminoides, and Tomato apical stunt viroid in Cestrum sp. Plant Pathology, 57, 399.Google Scholar
  19. Verhoeven, J. Th. J., Jansen, C. C. C., Roenhorst, J. W., Flores, R., & De la Peña, M. (2009). Pepper chat fruit viroid: biological and molecular properties of a proposed new species of the genus Pospiviroid. Virus Research, 144, 209–214.Google Scholar
  20. Verhoeven, J. Th. J., Jansen, C. C. C., Willemen, T. M., Kox, L. F. F., Owens, R. A., & Roenhorst, J. W. (2004). Natural infections of tomato by Citrus exocortis viroid, Columnea latent viroid, Potato spindle tuber viroid and Tomato chlorotic dwarf viroid. European Journal of Plant Pathology, 110, 823–831.Google Scholar
  21. Verhoeven, J. Th. J., Hüner, L., Viršček Marn, M., Mavrič Pleško, I., & Roenhorst, J. W. (2010). Mechanical transmission of Potato spindle tuber viroid between plants of Brugmansia suaveoles, Solanum jasminoides and potatoes and tomatoes. European Journal of Plant Pathology, 128, 417–421.Google Scholar
  22. Viršček Marn, M., Mavrič Pleško, I., & Gerič Stare, B. (2013). Variability of potato spindle tuber viroid isolates from ornamental hosts in Slovenia. Journal of Plant Pathology, 1, 411–415.Google Scholar

Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2014

Authors and Affiliations

  • Jasna Milanović
    • 1
  • Vesna Kajić
    • 1
  • Snježana Mihaljević
    • 2
  1. 1.Croatian Centre for Agriculture, Food and Rural AffairsInstitute for Plant ProtectionZagrebCroatia
  2. 2.Institute Ruđer BoškovićZagrebCroatia

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