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Differential necrotic lesion formation in soybean cultivars in response to soybean mosaic virus

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Abstract

In this study, we examined the necrosis phenotype on leaves of two cultivars of soybean (ZheA8901 and Nannong1138-2) that show varying level of resistance to soybean mosaic virus (SMV). The necrotic symptoms seen on inoculated and systemic leaves of soybean cultivar ZheA8901 were reminiscent of programmed cell death (PCD). The cell death phenotypes were evaluated using the TUNEL method, quantification of hydrogen peroxide (H2O2) and salicylic acid, callose production, as well as by monitoring expression of defence genes GmPR-1 and GmNPR1. Our results show that SMV inoculation induced PCD on ZheA8901 is associated with rapid increase in H2O2, increased SA and callose accumulation and higher defence gene expression.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant No. 31171574, 31101164), the National Soybean Industrial Technology System of China (No. CARS-004) and the Fund of Transgenic Breeding for Soybean Resistance to Soybean Mosaic Virus (No.2008ZX08004-004), the 111project (B08025) and PAPD.

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Authors and Affiliations

  1. National Center for Soybean Improvement; National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China

    Kai Zhang, Ying Pei Song, Yu Wang, Kai Li, Le Gao, Yong Kun Zhong, A. Karthikeyan & Hai Jian Zhi

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  1. Kai Zhang
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  2. Ying Pei Song
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  3. Yu Wang
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  4. Kai Li
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  5. Le Gao
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  6. Yong Kun Zhong
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  7. A. Karthikeyan
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  8. Hai Jian Zhi
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Correspondence to Hai Jian Zhi.

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Zhang, K., Song, Y.P., Wang, Y. et al. Differential necrotic lesion formation in soybean cultivars in response to soybean mosaic virus. Eur J Plant Pathol 139, 525–534 (2014). https://doi.org/10.1007/s10658-014-0408-7

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