Differential necrotic lesion formation in soybean cultivars in response to soybean mosaic virus
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In this study, we examined the necrosis phenotype on leaves of two cultivars of soybean (ZheA8901 and Nannong1138-2) that show varying level of resistance to soybean mosaic virus (SMV). The necrotic symptoms seen on inoculated and systemic leaves of soybean cultivar ZheA8901 were reminiscent of programmed cell death (PCD). The cell death phenotypes were evaluated using the TUNEL method, quantification of hydrogen peroxide (H2O2) and salicylic acid, callose production, as well as by monitoring expression of defence genes GmPR-1 and GmNPR1. Our results show that SMV inoculation induced PCD on ZheA8901 is associated with rapid increase in H2O2, increased SA and callose accumulation and higher defence gene expression.
KeywordsNecrosis Programmed cell death Salicylic acid SMV
This work was supported by the National Natural Science Foundation of China (Grant No. 31171574, 31101164), the National Soybean Industrial Technology System of China (No. CARS-004) and the Fund of Transgenic Breeding for Soybean Resistance to Soybean Mosaic Virus (No.2008ZX08004-004), the 111project (B08025) and PAPD.
- Kranthi, K. M., & Karen-Beth, G. S. (2013). Plant immune responses against viruses: how does a virus cause disease? Plant Cell, online.Google Scholar
- Miklós, P., Julia, K., Ingrid, H., Erich, F. E., & Balázs, B. (2004). Juvenility of tobacco induced by cytokinin gene introduction decreases susceptibility to Tobacco necrosis virus and confers tolerance to oxidative stress. Physiological and Molecular Plant Pathology, 65(2004), 39–47.Google Scholar
- Niderman, T., Genetet, I., Bruyère, T., Gees, R., Stintzi, A., Legrand, M., et al. (1995). Pathogenesis-related PR-1 proteins are antifungal. Isolation and characterization of three 14-kilodalton proteins of tomato and of a basic PR-1 of tobacco with inhibitory activity against Phytophthora infestans. Plant Physiology, 108(1), 17–27.PubMedCentralPubMedCrossRefGoogle Scholar
- Padder, B. A. (2014). Plant disease resistance genes: from perception to signal transduction. Plant Signaling: Understanding the Molecular Crosstalk, 20, 345–354.Google Scholar
- Seo, Y. S., Rojas, M. R., Lee, J. Y., Lee, S. W., Jeon, J. S., Ronald, P., et al. (2006). A viral resistance gene from common bean functions across plant families and is up-regulated in a non-virus-specific manner. Proceedings of the National Academy of Sciences of the United States of America, 103, 11856–11861.PubMedCentralPubMedCrossRefGoogle Scholar