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Genetic variability and virulence of Meloidogyne incognita populations from Brazil to resistant cotton genotypes

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Abstract

The root-knot nematode Meloidogyne incognita is widely distributed and a major pathogen of cotton (Gossypium spp.) worldwide. The objectives of this study were to assess the genetic variability and aggressiveness of Brazilian populations of M. incognita in cotton. Five populations of M. incognita and one isolate of M. enterolobii (outgroup) were used in the molecular analysis. Our results showed that only 2.7 % of the RAPD and AFLP fragments were polymorphic. Despite the existence of two races (races 3 and 4) and two esterase phenotypes (I1 and I2), a low genetic variability among populations was observed, which might be due to the mitotic parthenogenetic mode of reproduction of this pathogen. The aggressiveness/virulence among populations towards different cotton genotypes was also studied. None of the populations was virulent to the resistant cotton genotypes M-315 RNR, TX-25, CIR1343, Wild Mexican Jack Jones and CIR1348 (reproduction factor <1). Two populations of M. incognita from the states of Mato Grosso do Sul and Parana (Umuarama) (races 4 and 3, respectively) were highly aggressive to the susceptible control FM966 and virulent to the accessions LA-887 and Clevewilt-6 that showed moderate resistance to other populations tested.

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Acknowledgments

This work was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), EMBRAPA—Recursos Genéticos e Biotecnologia and EMBRAPA—Algodão. E.H. Silva and V.S. Mattos thank CNPq for their scholarships.

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Authors and Affiliations

  1. Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF, 70849-970, Brazil

    Esdras Henrique da Silva, Vanessa da Silva Mattos, Aldemiro Jorge-Junior, Valdir Ribeiro Correa & Regina Maria Dechechi Gomes Carneiro

  2. Departamento de Fitopatologia, Universidade de Brasília, Brasília, DF, 70910-900, Brazil

    Esdras Henrique da Silva, Vanessa da Silva Mattos, Cleber Furlaneto & Valdir Ribeiro Correa

  3. Núcleo Cerrado da Embrapa Algodão, Santo Antônio de Goiás, GO, 75375-000, Brazil

    Paulo Augusto Vianna Barroso

  4. Embrapa- Centro Nacional de Pesquisa em Hortaliças, Brasília, DF, 70359-970, Brazil

    Antônio Williams Moita

  5. Cirad, UMR AGAP, Avenue Agropolis, 34398, Montpellier, France

    Marc Giband

  6. INRA-UMR 1355, UNS, CNRS-UMR 7254, Institut Sophia Agrobiotech, 400 route des Chappes, BP167, 06903, Sophia Antipolis, France

    Philippe Castagnone-Sereno

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  1. Esdras Henrique da Silva
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  2. Vanessa da Silva Mattos
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  3. Cleber Furlaneto
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  4. Marc Giband
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  9. Philippe Castagnone-Sereno
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  10. Regina Maria Dechechi Gomes Carneiro
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Correspondence to Regina Maria Dechechi Gomes Carneiro.

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Esdras Henrique da Silva and Vanessa da Silva Mattos contributed equally to this work.

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da Silva, E.H., da Silva Mattos, V., Furlaneto, C. et al. Genetic variability and virulence of Meloidogyne incognita populations from Brazil to resistant cotton genotypes. Eur J Plant Pathol 139, 195–204 (2014). https://doi.org/10.1007/s10658-014-0381-1

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