Incidence of Bois Noir phytoplasma in different viticulture regions of Spain and Stolbur isolates distribution in plants and vectors
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The incidence of Bois Noir (BN) disease in grapevine plots, the population of the vector Hyalesthes obsoletus, and the distribution of stolbur phytoplasma isolates were studied over a 4 year period in five regions of Spain. BN incidence in affected plots ranged from 1 to 75 %. A study of the H. obsoletus population indicated that individuals of this insect vector were identified in most of the sampled plots with low populations, from 0.25–5 individuals per aspiration. The population peaks of H. obsoletus were reached at different dates between June 6th and July 24th, depending on the sampled zone and the year. The percentage of H. obsoletus individuals carrying the phytoplasma showed an average of 55 %. In Aragon, this percentage rose to 76 %. The strain tuf-b of stolbur phytoplasma was the most prevalent type in grapevine plants and H. obsoletus, except in grapevine plants from La Rioja where the strain tuf-a was detected in most plants. A study of the vmp1 gene revealed the presence of four different isolates in grapevine plants and H. obsoletus.
KeywordsStolbur Hyalesthes Phytoplasma strains Bois Noir Grapevine
We would like to thank J. Legorburu from Neiker (Vitoria-Gasteiz), J. Fortanete and A. Sorolla from Centro de Protección Vegetal (Zaragoza), J.J. Perez de Obanos and L. Caminero from EVENA (Navarra), J.L. Perez Marin from CIDA-La Rioja, and J. Reyes and G. Barrios from DAAM- Servei Sanitat Vegetal (Catalonia) for their help in conducting the surveys.
This work was funded by grant RTA11-067-C4 of the Programa Sectorial de I + D, M.A.P.A., Spain.
- Langer, M., & Maixner, M. (2004). Molecular characterization of grapevine yellows associated phytoplasmas of the stolbur-group based on RFLP-analysis of non-ribosomal DNA. Vitis, 43(4), 191–199.Google Scholar
- Laviña, A., Batlle, A., Larrue, J., Daire, X., Clair, D., & Boudon-Padieu, E. (1995). First report of grapevine bois noir phytoplasma in Spain. Plant Disease, 79, 1075.Google Scholar
- Lessio, M., Tedeschi, R., & Alma, A. (2007). Population dynamics, host plants and infection rate with stolbur phytoplasma of Hyalesthes obsoletus Signoret in North-Western Italy. Journal of Plant Pathology, 89(1), 97–102.Google Scholar
- Maixner, M. (1994). Research note: transmission of German grapevine yellows (Vergilbungskrankheit) by the planthopper Hyalesthes obsoletus (Auchenorrhyncha: Cixiidae). Vitis, 33, 103–104.Google Scholar
- Maixner, M., Johannesen, J., & Seitz, A. (2009). Aspects of the interaction of stolbur phytoplasma, vectors and host plants in the two epidemic systems of Bois Noir. Proceedings of 16th Meeting of ICVG. France: Dijon.Google Scholar
- Mori, N., Pavan, F., Bondavalli, R., Reggiani, N., Paltrinieri, S., & Bertaccini, A. (2007). Factors affecting the spread of “bois noir” disease in grapevine cv. Lambrusco in northern Italy. Bulletin of Insectology, 60, 295–296.Google Scholar
- Palermo, S., Elekes, M., Botti, S., Ember, I., Alma, A., Orosz, A., et al. (2004). Presence of stolbur phytoplasma in Cixiidae in Hungarian vineyards. Vitis, 43, 201–203.Google Scholar
- Quaglino, F., Zhao, Y., Casati, P., Bulgari, D., Bianco, P.A., Wei, W.et al. (2013). ‘Candidatus Phytoplasma solani’, a novel taxon associated with stolbur and bois noir related diseases of plants. International Journal Systematic Evolutionary Microbiology. PMID:23334879.Google Scholar
- Sabaté, J., Laviña, A. & Batlle, A. (2006). Molecular characterization of stolbur phytoplasma isolates in grapevines and insect vectors. 15th ICGV Symposium, Stellenbosch (South Africa). Extended Abstracts pp-157–158.Google Scholar
- Sabaté, J., Laviña, A., Legorburu, J., Fortanete, J., de Pérez Obanos, J. J., Pérez Marín, J. L., et al. (2007). Incidence of Bois Noir phytoplasma in different wine-growing regions of Spain and its relation to Hyalesthes obsoletus. Bulletin of Insectology, 60(2), 367–368.Google Scholar
- Smart, C. D., Schneider, B., Blomquist, C. L., Guerra, L. J., Harrison, N. A., Ahrens, U., et al. (1996). Phytoplasma-specific PCR primers based on sequences of the 16S-23S rRNA spacer region. Applied Environmental Microbiology, 62, 1988–1993.Google Scholar
- Trivellone, V., Pinzauti, F., & Bagnoli, B. (2005). Reptalus quinquecostatus (Dufour) (Auchenorrhyncha Cixiidae) as a possible vector of Stolbur-phytoplasma in a vineyard in Tuscany. Redia, 88, 103–108.Google Scholar