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Mapping of QTL lengthening the latent period of Puccinia striiformis in winter wheat at the tillering growth stage

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Abstract

The winter wheat lines Luke and AQ24788-83 are respectively susceptible and slow-rusting at tillering stage to yellow (stripe) rust, caused by Puccinia striiformis f. sp. tritici (Pst). A mapping population consisting of 206 recombinant inbred lines was developed from the cross Luke × AQ24788-83. These lines were evaluated at the tillering stage in the field trials for infection type (IT) and disease incidence (DI) and in greenhouse trials for IT and latent period (LP). A significant negative correlation was found between LP and DI. A genetic map with 473 marker loci was constructed and used for identifying QTL associated with LP and IT. Two QTL, QYr.cau-1BS and QYr.cau-5AS, were mapped on 1BS and 5AS respectively, explaining collectively up to 46.4 % of LP phenotypic variance. QYr.cau-5AS was clearly distinct, in terms of mapping position, from all six yellow rust resistance genes/QTL previously reported on 5A. QYr.cau-1BS could not be spatially differentiated from three (i.e. YrAlp, Yr15, and YrH52) of the six genes/QTL known on 1BS and centromere-vicinity regions, but was determined to be different from these three genes based on phenotype. The two QTL identified here, therefore, are likely to be novel to the currently known Pst resistance genes/QTL. A minor QTL on 3AL was detected to be associated with both IT and LP. Expression of quantitative resistance at early wheat growth stages and usefulness of the QTL are discussed for the wheat-Pst system.

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Abbreviations

1BS:

The short arm of chromosome 1B

5AS:

The short arm of chromosome 5A

CIM:

Composite interval mapping

DI:

Disease incidence

cM:

centiMorgans

IT:

Infection type

LOD:

Logarithm of the odds

LP:

Latent period

MIM:

Multiple interval mapping

Pst :

Puccinia striiformis Westend. f. sp. tritici Eriks.

QTL:

Quantitative trait locus or quantitative loci, depending on context

RIL:

Recombinant inbred line

SSR:

Simple sequence repeat, microsatellite

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Acknowledgment

This study was supported by the National Natural Science Foundation of China (30871612), the Commonweal Specialized Research Fund of China Agriculture (200903035), and Program for Changjiang Scholars and Innovative Research Team (IRT1042).

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Authors and Affiliations

  1. Department of Plant Pathology, China Agricultural University West Campus, No.2 Yuan Ming Yuan West Road, Beijing, 100193, People’s Republic of China

    Wei Quan, Guangliang Hou, Jiang Chen, Ziyi Du, Feng Lin, Yan Guo, Song Liu & Zhongjun Zhang

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  1. Wei Quan
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  2. Guangliang Hou
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  3. Jiang Chen
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Correspondence to Zhongjun Zhang.

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Quan, W., Hou, G., Chen, J. et al. Mapping of QTL lengthening the latent period of Puccinia striiformis in winter wheat at the tillering growth stage. Eur J Plant Pathol 136, 715–727 (2013). https://doi.org/10.1007/s10658-013-0201-z

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