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European Journal of Plant Pathology

, Volume 136, Issue 4, pp 715–727 | Cite as

Mapping of QTL lengthening the latent period of Puccinia striiformis in winter wheat at the tillering growth stage

  • Wei Quan
  • Guangliang Hou
  • Jiang Chen
  • Ziyi Du
  • Feng Lin
  • Yan Guo
  • Song Liu
  • Zhongjun Zhang
Article

Abstract

The winter wheat lines Luke and AQ24788-83 are respectively susceptible and slow-rusting at tillering stage to yellow (stripe) rust, caused by Puccinia striiformis f. sp. tritici (Pst). A mapping population consisting of 206 recombinant inbred lines was developed from the cross Luke × AQ24788-83. These lines were evaluated at the tillering stage in the field trials for infection type (IT) and disease incidence (DI) and in greenhouse trials for IT and latent period (LP). A significant negative correlation was found between LP and DI. A genetic map with 473 marker loci was constructed and used for identifying QTL associated with LP and IT. Two QTL, QYr.cau-1BS and QYr.cau-5AS, were mapped on 1BS and 5AS respectively, explaining collectively up to 46.4 % of LP phenotypic variance. QYr.cau-5AS was clearly distinct, in terms of mapping position, from all six yellow rust resistance genes/QTL previously reported on 5A. QYr.cau-1BS could not be spatially differentiated from three (i.e. YrAlp, Yr15, and YrH52) of the six genes/QTL known on 1BS and centromere-vicinity regions, but was determined to be different from these three genes based on phenotype. The two QTL identified here, therefore, are likely to be novel to the currently known Pst resistance genes/QTL. A minor QTL on 3AL was detected to be associated with both IT and LP. Expression of quantitative resistance at early wheat growth stages and usefulness of the QTL are discussed for the wheat-Pst system.

Keywords

DNA marker Quantitative resistance Slow rusting Stripe rust Triticum aestivum L. 

Abbreviations

1BS

The short arm of chromosome 1B

5AS

The short arm of chromosome 5A

CIM

Composite interval mapping

DI

Disease incidence

cM

centiMorgans

IT

Infection type

LOD

Logarithm of the odds

LP

Latent period

MIM

Multiple interval mapping

Pst

Puccinia striiformis Westend. f. sp. tritici Eriks.

QTL

Quantitative trait locus or quantitative loci, depending on context

RIL

Recombinant inbred line

SSR

Simple sequence repeat, microsatellite

Notes

Acknowledgment

This study was supported by the National Natural Science Foundation of China (30871612), the Commonweal Specialized Research Fund of China Agriculture (200903035), and Program for Changjiang Scholars and Innovative Research Team (IRT1042).

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Copyright information

© KNPV 2013

Authors and Affiliations

  • Wei Quan
    • 1
  • Guangliang Hou
    • 1
  • Jiang Chen
    • 1
  • Ziyi Du
    • 1
  • Feng Lin
    • 1
  • Yan Guo
    • 1
  • Song Liu
    • 1
  • Zhongjun Zhang
    • 1
  1. 1.Department of Plant PathologyChina Agricultural University West CampusBeijingPeople’s Republic of China

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