Mapping of QTL lengthening the latent period of Puccinia striiformis in winter wheat at the tillering growth stage
- 284 Downloads
The winter wheat lines Luke and AQ24788-83 are respectively susceptible and slow-rusting at tillering stage to yellow (stripe) rust, caused by Puccinia striiformis f. sp. tritici (Pst). A mapping population consisting of 206 recombinant inbred lines was developed from the cross Luke × AQ24788-83. These lines were evaluated at the tillering stage in the field trials for infection type (IT) and disease incidence (DI) and in greenhouse trials for IT and latent period (LP). A significant negative correlation was found between LP and DI. A genetic map with 473 marker loci was constructed and used for identifying QTL associated with LP and IT. Two QTL, QYr.cau-1BS and QYr.cau-5AS, were mapped on 1BS and 5AS respectively, explaining collectively up to 46.4 % of LP phenotypic variance. QYr.cau-5AS was clearly distinct, in terms of mapping position, from all six yellow rust resistance genes/QTL previously reported on 5A. QYr.cau-1BS could not be spatially differentiated from three (i.e. YrAlp, Yr15, and YrH52) of the six genes/QTL known on 1BS and centromere-vicinity regions, but was determined to be different from these three genes based on phenotype. The two QTL identified here, therefore, are likely to be novel to the currently known Pst resistance genes/QTL. A minor QTL on 3AL was detected to be associated with both IT and LP. Expression of quantitative resistance at early wheat growth stages and usefulness of the QTL are discussed for the wheat-Pst system.
KeywordsDNA marker Quantitative resistance Slow rusting Stripe rust Triticum aestivum L.
The short arm of chromosome 1B
The short arm of chromosome 5A
Composite interval mapping
Logarithm of the odds
Multiple interval mapping
Puccinia striiformis Westend. f. sp. tritici Eriks.
Quantitative trait locus or quantitative loci, depending on context
Recombinant inbred line
Simple sequence repeat, microsatellite
This study was supported by the National Natural Science Foundation of China (30871612), the Commonweal Specialized Research Fund of China Agriculture (200903035), and Program for Changjiang Scholars and Innovative Research Team (IRT1042).
- Basten, J. C., Wang, S., Gaffney, P., & Zeng, Z. B. (2003). Windows QTL cartographer, Version 2.0, statistical genetics. Raleigh: North Carolina State University.Google Scholar
- de Vallavieille-Pope, C., Huber, L., Leconte, M., & Goyeau, H. (1995). Comparative effects of temperature and interrupted wet periods on germination, penetration, and infection of Puccinia recondita f. sp. tritici and P. striiformis on wheat seedlings. Phytopathology, 85, 409–415.CrossRefGoogle Scholar
- Lowe, I., Jankuloski, L., Chao, S., Chen, X. M., See, D., & Dubcovsky, J. (2011). Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat. Theoretical and Applied Genetics, 123, 143–157.PubMedCrossRefGoogle Scholar
- Naz, A. A., Kunert, A., Flath, K., Pillen, K., & Léon, J. (2012). Advanced backcross quantitative trait locus analysis in winter wheat: dissection of stripe rust seedling resistance and identification of favorable exotic alleles originated from a primary hexaploid wheat (Triticum turgidum ssp. dicoccoides × Aegilops tauschii). Molecular Breeding. doi: 10.1007/s11032-012-9710-2.Google Scholar
- Peng, J. H., Fahima, T., Röder, M. S., Li, Y. C., Grama, A., & Nevo, E. (2000). Microsatellite high-density mapping of the stripe rust resistance gene YrH52 region on chromosome 1B and evaluation of its marker-assisted selection in the F2 generation in wild emmer wheat. New Phytologist, 146, 141–154.CrossRefGoogle Scholar
- Rosewarne, G. M., Singh, R. P., Huerta-Espino, J., Herrera-Foessel, S. A., Forrest, K. L., Hayden, M. J., et al. (2012). Analysis of leaf and stripe rust severities reveals pathotype changes and multiple minor QTLs associated with resistance in an Avocet × Pastor wheat population. Theoretical and Applied Genetics, 124, 1283–1294.PubMedCrossRefGoogle Scholar
- Stubbs, R. W. (1988). Pathogenicity analysis of yellow (stripe) rust of wheat and its significance in a global context. In N. W. Simmonds & S. Rajaram (Eds.), Breeding strategy for resistance to the rusts of wheat (pp. 23–38). Mexico: CIMMYT.Google Scholar