European Journal of Plant Pathology

, Volume 136, Issue 3, pp 597–602 | Cite as

Evaluation of Malus germplasm resistance to marssonina apple blotch

  • Lihua Yin
  • Mingjun Li
  • Xiwang Ke
  • Cuiying Li
  • Yangjun Zou
  • Dong Liang
  • Fengwang Ma


Marssonina apple blotch, caused by Diplocarpon mali (anamorph: Marssonina coronaria), is a serious disease of apple (Malus x domestica Borkh.). We evaluated the resistance of 28 cultivars and 39 species or biotypes after inoculating leaves with conidial suspensions in vitro and in vivo. Five accessions of the 28 apple cultivars showed a good level of resistance, while 16 displayed intermediate type. In contrast, resistance was good for eight accessions of species or biotypes and moderate for 19 accessions. Among these 67 candidates, more than 19 % had resistance against Marssonina apple blotch, indicating that resistant resources are abundant in that germplasm. These high-performing accessions can make valuable contributions to breeding programs for resistance against this disease.


Artificial inoculation Diplocarpon mali Resistance breeding Screening method 



days post-inoculation


mean length of lesions of each accession in vitro


mean leaf area showing lesions of each accession in vivo



This work was supported by the earmarked fund for the China Agriculture Research System. The authors are grateful to Priscilla Licht for help in revising our English composition.


  1. Chen, X. S., Han, M. Y., Su, G. L., Liu, F. Z., Guo, N., Jiang, Y. M., et al. (2010). Discussion on today’s world apple industry trends and the suggestions on sustainable and efficient development of apple industry in China. Journal of Fruit Science, 27, 594–604.Google Scholar
  2. Gao, Y. E., Li, B. H., Dong, X. L., Wang, C. X., Li, G. F., & Li, B. D. (2011). Effects of temperature and moisture on sporulation of Diplocarpon mali on overwintered apple leaves. Scientia Agricultura Sinica, 44, 1367–1374.Google Scholar
  3. Gessler, C., & Pertot, I. (2012). Vf scab resistance of Malus. Trees, 26, 95–108.CrossRefGoogle Scholar
  4. Harada, Y., Sawamura, K., & Konno, K. (1974). Diplocarpon mali sp. Nov., the perfect state of apple blotch fungus Marssonina coronaria. Annals of the Phytopathological Society of Japan, 40, 412–418.CrossRefGoogle Scholar
  5. Lee, S. W., Suh, S. J., Kim, D., Choi, K. H., Lee, D. H., & Ryu, O. H. (1996). Questionnaire on status and opinions on pest control for apple growers and related groups. RDA Journal of Agricultural Science, Crop Protection, 38, 545–552.Google Scholar
  6. Lee, D. H., Back, C. G., Nang, K. K. W., Choi, K. H., Kim, K. M., & Kang, I. K. (2011). Biological characterization of Marssonina coronaria associated with apple blotch disease. Mycobiology, 39, 200–205.PubMedCrossRefGoogle Scholar
  7. Leite Junior, R. P., Tsuneta, M., & Kishino, A. Y. (1986). Apple leaf spot caused by Marssonina coronaria. Fitopatologia Brasileira, 3, 725–729.Google Scholar
  8. Li, Y., Zhou, Q., Gao, H., Wan, Y. Z., Wang, L. C., & Zhao, Z. Y. (2012). Evaluation of resistance to Marssonina coronaria and Alternaria mali of major apple cultivars. Journal of Northwest Forestry University, 27, 132–136.Google Scholar
  9. Noiton, D. A. M. (1996). Founding clones, inbreeding, coancestry, and status number of modern apple cultivars. Journal of the American Society for Horticultural Science, 121, 773–782.Google Scholar
  10. Parmelee, J. A. (1971). Marssonina leaf spot of apple. Canadian Plant Disease Survey, 51, 91–92.Google Scholar
  11. Sagong, D. H., Kweon, H. J., Song, Y. Y., Park, M. Y., Nam, J. C., Kang, S. B., et al. (2011). Influence of defoliation by Marssonina blotch on vegetative growth and fruit quality in ‘Fuji’/M.9 apple tree. Korean Journal of Horticultural Science & Technology, 29, 531–538.Google Scholar
  12. Sharma, J. N. (1999). Marssonina blotch—a new disease causing premature leaf fall in apple. Indian Phytopathology, 52, 101–102.Google Scholar
  13. Sharma, J. N., Sharma, A., & Sharma, P. (2004). Out-break of Marssonina blotch in warmer climates causing premature leaf fall problem of apple and its management. Acta Horticulturae, 662, 405–409.Google Scholar
  14. Sharma, N., Thakur, V. S., Mohan, J., Khurana, S. M. P., & Sharma, S. (2009). Epidemiology of Marssonina blotch (Marssonina coronaria) of apple in India. Indian Phytopathology, 62, 348–359.Google Scholar
  15. Sharma, N., Thakur, V. S., Sharma, S., Mohan, J., & Khurana, S. M. P. (2011). Development of Marssonina blotch (Marssonina coronaria) in different genotypes of apple. Indian Phytopathology, 64, 358–362.Google Scholar
  16. Shou, Y. Y., Li, C. M., Zhao, Y. B., Chen, D. M., & Zhang, X. Z. (2009). In vitro evaluation of resistance to Marssonina mali in apple. Journal of Fruit Science, 26, 912–914.Google Scholar
  17. Tamietti, G., & Matta, A. (2003). First report of leaf blotch caused by Marssonina coronaria on apple in Italy. Plant Disease, 87, 1005.CrossRefGoogle Scholar
  18. Tanaka, S., Kamegawa, N., Ito, S., & Kameya-Iwaki, M. (2000). Detection of thiophanate-methyl-resistant strains in Diplocarpon mali, causal fungus of apple blotch. Joural of General Plant Pathology, 66, 82–85.CrossRefGoogle Scholar
  19. Thakur, V. S., & Sharma, N. (2010). Epidemic outbreak of apple blotch disease: epidemiology and management in Himachal Pradesh. Indian Phytopathology, 63, 141–144.Google Scholar
  20. Verma, K. D., & Sharma, J. N. (2003). Scab and Marssonina blotch-threatening diseases of apple and their management. Annual Review of Plant Pathology, 2, 207–220.Google Scholar
  21. Xie, W. L., & Leng, H. Q. (1990). Studies on apple blotch 2: relation between the resistance of apple cultivars and their physical and physiological characters. Sichuan Agriculture University, 8, 51–54.Google Scholar
  22. Zhao, H., Zhou, T. C., Cheng, J. J., Li, X. H., & Huang, L. L. (2009a). Control effect of triazole fungicides in controlling Marssonina coronaria in vitro and in field. Scientia Silvae Sinicae, 45, 68–73.Google Scholar
  23. Zhao, H., Huang, L. L., Xie, F. Q., & Kang, Z. S. (2009b). Culture study of Marssonina coronaria from diseased apple leaves. Mycosystema, 28, 490–495.Google Scholar
  24. Zhao, H., Huang, L. L., Xiao, C. L., Liu, J., Wei, J., & Gao, X. N. (2010). Influence of culture media and environmental factors on mycelial growth and conidial production of Diplocarpon mali. Letters in Applied Microbiology, 50, 639–644.PubMedCrossRefGoogle Scholar
  25. Zhou, T. C., Zhao, H., Huang, L. L., Xi, H. M., Zhou, D. Z., & Cheng, J. J. (2008). Efficacy of propineb for controlling leaf blotch caused by Marssonina coronaria and its effect on zinc content in apple leaves. Acta Phytophylacica Sinica, 35, 519–524.Google Scholar

Copyright information

© KNPV 2013

Authors and Affiliations

  • Lihua Yin
    • 1
  • Mingjun Li
    • 1
  • Xiwang Ke
    • 1
  • Cuiying Li
    • 1
  • Yangjun Zou
    • 1
  • Dong Liang
    • 1
  • Fengwang Ma
    • 1
  1. 1.State Key Laboratory of Crop Stress Biology for Arid Areas, College of HorticultureNorthwest A&F UniversityShaanxiChina

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