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Competence of Frankliniella occidentalis and Frankliniella intonsa strains as vectors for Chrysanthemum stem necrosis virus

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Abstract

The vector competence of Frankliniella occidentalis for Chrysanthemum stem necrosis virus (CSNV) was evaluated. Three vector strains with distinct competences for Tomato spotted wilt virus (TSWV) transmission were investigated, including an artificially selected strain (TsH) that has a particularly high competence (>90 %). Newly hatched larvae of F. occidentalis were given an acquisition access period of 5 days on CSNV-infected D. stramonium leaves, and reared to maturity. Their transmission efficiencies were examined using a leaf disk assay using Petunia x hybrida leaves. Following the leaf disk assay, the virus accumulation in the vectors was examined via a double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) of their bodies. The results showed that the CSNV acquisition and transmission efficiency of the TsH strain did not differ from those of the others, indicating that the competence of F. occidentalis as a vector for CSNV is not related to that for TSWV. The CSNV transmission and acquisition efficiencies of two F. intonsa strains (Hiroshima and Fukuoka) were also evaluated. In Hiroshima strain, 35 % of adults were viruliferous, but only two transmitters (3 %) were observed. In Fukuoka strain, 6 % were viruliferous, and no transmitters were observed. These results indicate that F. intonsa cannot be a major vector for CSNV. The accumulation of CSNV in the adults of F. occidentalis and F. intonsa evaluated using DAS-ELISA showed a significant difference in ELISA values among transmitter, viruliferous non-transmitter, and non-viruliferous individuals. These results clearly demonstrated that only transmitters that accumulated a threshold quantity of virus can transmit CSNV to plants.

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Acknowledgement

The authors would like to thank Dr. Tamito Sakurai and Dr. Tomotoshi Kashio for providing F. occidentalis and F. intonsa strains and for their technical advice on the maintenance of thrips strains.

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Authors and Affiliations

  1. The United Graduate School of Agricultural Sciences, Kagoshima University, Korimoto, Kagoshima, 890-0065, Japan

    Shiori Okuda & Hisashi Iwai

  2. Ministry of Agriculture, Forestry and Fisheries, Chiyoda-ku, Tokyo, 100-8950, Japan

    Mitsuru Okuda

  3. Hiroshima Prefectural Agriculture Research Center, Hachihonmatsu, Higashi-hiroshima, Hiroshima, 739-0151, Japan

    Shohei Matsuura

  4. Oita Prefectural Agriculture, Forestry and Fisheries Research Center, Bungo-ohno, Oita, 879-7111, Japan

    Shinichiro Okazaki

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  1. Shiori Okuda
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  2. Mitsuru Okuda
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  3. Shohei Matsuura
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  4. Shinichiro Okazaki
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  5. Hisashi Iwai
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Correspondence to Mitsuru Okuda or Hisashi Iwai.

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Okuda, S., Okuda, M., Matsuura, S. et al. Competence of Frankliniella occidentalis and Frankliniella intonsa strains as vectors for Chrysanthemum stem necrosis virus . Eur J Plant Pathol 136, 355–362 (2013). https://doi.org/10.1007/s10658-013-0169-8

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