Development of Fusarium langsethiae in commercial cereal production
- 394 Downloads
A field survey was performed to study the infection and development of Fusarium langsethiae in the growing season of wheat, barley, oats and triticale under commercial (2009 – 2011) production. Sampling was completed over three years from fields within the counties of Shropshire and Staffordshire in the UK. Plants sampled (from tillering to harvest) were divided into roots, leaves, lower stem, upper stem and inflorescence/head sub-samples depending on the growth stage of the cereal. DNA was extracted and F. langsethiae DNA quantified using real-time PCR. Fusarium mycotoxins HT-2 and T-2 were quantified from head samples at harvest. Three years of data showed oat to contain the highest levels of both F. langsethiae biomass and HT-2 + T-2 mycotoxins in harvested heads of the cereals studied. The development of F. langsethiae in all three cereals appeared to be similar. Fusarium langsethiae DNA was not detected in the roots and seedlings of all three cereals suggesting F. langsethiae is not a seedling pathogen of cereals. Head infection if it occurs, is at head emergence but before flowering. Seemingly symptomless heads had high levels of F. langsethiae DNA and HT-2 + T-2, confirming previous suggestions that F. langsethiae is a symptomless pathogen of oats.
KeywordsField survey Head blight Head infection Real-time PCR Symptomless infection
The first author acknowledges funding of a PhD studentship from HGCA-AHDB and Harper Adams University.
- Agrios, N.G. (2005). Plant pathology. 5th ed. Elsevier Academic Press.Google Scholar
- Bocarov-Stancic, A., Levic, J., Stankovic, S., Tancic, S., Krnjaja, V., & Salma, N. (2008). Toxigenic potential of Fusarium langsethiae isolates from Serbian wheat kernels. Cereal Research Communications, 36, 345–346.Google Scholar
- De Wolf, E., Madden, L., & Lipps, P. (2000). Prediction of Fusarium head blight epidemics. 2000 National Fusarium Head Blight Forum (pp. 131–135). http://www.scabusa.org/forum-00-proc.pdf.
- Edwards, S.G. (2007). Investigation of Fusarium mycotoxins in UK barley and oat production. HGCA Project Report No. 415. HGCA, London.Google Scholar
- Edwards S. G., Imathiu S. M., Ray R. V., Back M & Hare M. C. (2012). Molecular studies to identify the Fusarium species responsible for HT-2 and T-2 mycotoxins in UK oats. International Journal of Food Microbiology, 156, 168–175.Google Scholar
- Fredlund, E., Gidlund, A., Pettersson, H., Olsen, M., & Borjesson, T. (2010). Realtime PCR detection of Fusarium species in Swedish oats and correlation to T-2 and HT-2 toxin content. World Mycotoxin Journal, 3, 77–88.Google Scholar
- Kosova, K., Chrpova, J. & Sip, V. (2009). Cereal resistance to fusarium head blight and possibilities of its improvement through breeding – a review. Czech Journal of. Genetics and Plant Breeding, 45, 87–105.Google Scholar
- Langseth, W., & Rundberget, T. (1999). The occurrence of HT-2 toxin and other trichothecenes in Norwegian cereals. Mycopathologia, 147, 157–165.Google Scholar
- McMullen, M., Bergstrom, G., De Wolf, E., Dill-Macky, R., Hershman, D., Shaner, G., & Van Sanford, D. A. (2012). Unified Effort to Fight an Enemy of Wheat and Barley: Fusarium Head Blight. Plant Disease, 96, 1712–1728.Google Scholar
- Opoku N., Back M. & Edwards S. G. (2011). Aggressiveness of Fusarium langsethiae isolates towards wheat, barley and oats in an in vitro leaf assay. Plant Breeding and Seed Science, 64, 55–63.Google Scholar
- White, T. J., Bruns, T., Lee, S. & Taylor, J. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In Innis, M. A., Gelfand, D. H. Sninsky, J. J. & White, T. J. (Eds.), PCR Protocols: A guide to methods and applications (pp. 315–322). Academic Press.Google Scholar
- Wilson, A., Simpson, D., Chandler, E., Jennings, P. & Nicholson, P. (2004). Development of PCR assays for the detection and differentiation of Fusarium sporotrichioides and Fusarium langsethiae. FEMS Microbiology Letters, 233, 69–76.Google Scholar
- Xu, X. M., Parry, D. W., Nicholson, P., Thomsett, M. A., Simpson, D., Edwards, S. G., Cooke, B. M., Doohan, F. M., Brennan, J. M., Moretti, A., Tocco, G., Mule, G., Hornok, L., Giczey, G., & Tatnell, J. (2005). Predominance and association of pathogenic fungi causing Fusarium ear blight in wheat in four European countries. European Journal of Plant Pathology, 112, 143–154.CrossRefGoogle Scholar
- Xu, X. M., Nicholson, P., Thomsett, M. A., Simpson, D., Cooke, B. M., Doohan, F. M., Brennan, J., Monaghan, S., Moretti, A., Mule, G., Hornok, L., Beki, E., Tatnell, J., Ritieni, A., & Edwards, S. G. (2008). Relationship between the fungal complex causing Fusarium head blight of wheat and environmental conditions. Phytopathology, 98, 69–78.PubMedCrossRefGoogle Scholar
- Yli-Mattila, T., Parikka, P., Lahtinen, T., Ramo, S., Kokkonen, M., Rizzo, A., Jestoi, M. & Hietaniemi, V. (2009). Fusarium DNA levels in Finish cereal grains. In Gherbawy, Y., Mach, R.L. & Rai, M.K. (Ed), Current advances in molecular mycology (pp 107–138). Nova Science Publishers.Google Scholar