European Journal of Plant Pathology

, Volume 135, Issue 2, pp 415–427 | Cite as

The role of the mealybug Phenacoccus aceris in the spread of Grapevine leafroll-associated virus −1 (GLRaV-1) in two French vineyards

  • Jean Le Maguet
  • Jean-Jacques Fuchs
  • Joël Chadœuf
  • Monique Beuve
  • Etienne Herrbach
  • Olivier Lemaire


Spread patterns of a Grapevine leafroll-associated virus 1 (GLRaV-1) epidemic and a mealybug infestation survey over 10 year were recorded in two Burgundy French vineyards to investigate the relation between them. The temporal evolution of leafroll spread at both study sites was compared on disease incidence data with logistic regression models. We first tested if the spatial distribution of the disease and the mealybug were aggregated using permutation methods, then we tested the independence between the two spatial patterns by randomly shifting one pattern. In Bonzon, an increase from 5 % to 86 % of leafroll prevalence was observed over an 8-year time span, whereas leafroll prevalence remained stable around 5 % in Marsannay-la-Côte during the same period. In Bonzon, the disease spread rapidly from older neighbouring vineyards in four main patches while no spread of the disease was recorded from infected vines in Marsannay-la-Côte. The mealybug Phenacoccus aceris was recorded on 74 % of vines in Bonzon throughout the study and only 6 % of vines in Marsannay-la-Côte. In the latter location, the disease was not associated with the presence of the mealybug, so that it may have arisen from infected plant material escaping the sanitary inspection. In Bonzon, the significant statistical correlation between the mealybug distribution and diseased plants suggests that P. aceris was responsible for the rapid spread of GLRaV-1 in the vineyard. This is the first report of GLRaV-1 natural spread in Europe.


Epidemiology Ampelovirus Grapevine scale insects Leafroll disease Spatial statistics 



Grapevine leafroll-associated virus


Grapevine virus A


Grapevine virus B


Double-antibody sandwich enzyme-linked immunosorbent assay



We thank Dr. Boudon-Padieu, Mr. Dureuil, and the winegrowers of Bonzon and Marsannay-la-Côte, Mr. Barraud and Mr. Charlopin for having initiated the leafroll survey. We are most grateful to Dr. René Sforza for his critical reading of the manuscript. This research was financed by three French professional committees for viticulture: Comité Interprofessionnel du Vin de Champagne, Epernay; Bureau Interprofessionnel des Vins de Bourgogne, Beaune; and Comité Interprofessionnel des Vins d’Alsace, Colmar; and by FranceAgriMer grants (N° 2009 47 005 104, N°2009 77 030 104 and N°2011 0106 001 104).

Supplementary material

10658_2012_99_MOESM1_ESM.ppt (822 kb)
ESM 1 (PPT 822 kb)


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Copyright information

© KNPV 2012

Authors and Affiliations

  • Jean Le Maguet
    • 1
    • 2
    • 3
  • Jean-Jacques Fuchs
    • 3
  • Joël Chadœuf
    • 4
  • Monique Beuve
    • 1
    • 3
  • Etienne Herrbach
    • 1
    • 3
  • Olivier Lemaire
    • 1
    • 3
  1. 1.INRA UMR 1131 Santé de la Vigne et Qualité du VinColmarFrance
  2. 2.Comité Interprofessionnel du Vin de Champagne (CIVC)EpernayFrance
  3. 3.Université de Strasbourg (UDS)StrasbourgFrance
  4. 4.INRA UR 546 Biostatistique et Processus SpatiauxAvignonFrance

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