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European Journal of Plant Pathology

, Volume 135, Issue 1, pp 35–47 | Cite as

Changes induced by powdery mildew in the salicylic acid and polyamine contents and the antioxidant enzyme activities of wheat lines

  • Magda Pál
  • Viktória Kovács
  • Gyula Vida
  • Gabriella Szalai
  • Tibor Janda
Article

Abstract

Investigations were made on four wheat (Triticum aestivum L.) lines under greenhouse conditions, in order to reveal the role of stress-protective materials, namely salicylic acid, polyamines and antioxidant enzymes in the level of tolerance to powdery mildew infection caused by Blumeria graminis (DC.) Speer f.sp. tritici Ém. Marchal. The four lines showed different levels of tolerance, assessed on the Saari-Prescott scoring scale: TC26 and TC33 proved to be susceptible and TC9 and TC19 resistant. In most of the lines, infection caused changes in the activities of antioxidant enzymes, especially in the case of guaiacol peroxidase. Four peroxidase isoenzymes, which responded differently to powdery mildew infection could be detected by gel electrophoresis. Infection had only a slight effect on the levels of salicylic acid (free and bound forms) in inoculated plants; while the levels of polyamines, especially spermidine and spermine increased after infection. Correlation analysis was also performed to examine how close a relationship exists between the parameters investigated. It was concluded that salicylic acid, polyamines and antioxidant enzymes have an important role in plant responses and defence mechanisms during this biotic stress and that in some cases there were significant relationships between them. However the levels of these compounds either initially or after pathogen inoculation, could not explain the degree of tolerance to powdery mildew in the four wheat lines investigated.

Keywords

Antioxidant enzyme Biotic stress Blumeria graminis tritici Polyamine Salicylic acid Triticum aestivum L. 

Abbreviations

APX

Ascorbate peroxidase

CAD

Cadaverine

GR

Glutathione reductase

GST

Glutathione-S-transferase

G-POD

Guaiacol peroxidase

oANI

Ortho-anisic acid

PAs

Polyamines

pHBA

Para-hydroxy-benzoic acid

PS II

Photosystem II

PUT

Putrescine

SA

Salicylic acid

SPD

Spermidine

SPN

Spermine

TCA

Trichloroacetic acid

Notes

Acknowledgements

The authors are gratefully indebted to Zsuzsa Kóti and Edit Kövesdi for their technical assistance. Thanks are also due to Barbara Hooper for revising the manuscript linguistically. This work was supported by a grant from the Hungarian National Scientific Research Foundation (OTKA PD83840), which is gratefully acknowledged. Magda Pál is a recipient of the János Bolyai Scholarship.

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Copyright information

© KNPV 2012

Authors and Affiliations

  • Magda Pál
    • 1
  • Viktória Kovács
    • 1
  • Gyula Vida
    • 1
  • Gabriella Szalai
    • 1
  • Tibor Janda
    • 1
  1. 1.Agricultural Institute, Centre of Agricultural Research, Hungarian Academy of SciencesMartonvásárHungary

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