Changes induced by powdery mildew in the salicylic acid and polyamine contents and the antioxidant enzyme activities of wheat lines
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Investigations were made on four wheat (Triticum aestivum L.) lines under greenhouse conditions, in order to reveal the role of stress-protective materials, namely salicylic acid, polyamines and antioxidant enzymes in the level of tolerance to powdery mildew infection caused by Blumeria graminis (DC.) Speer f.sp. tritici Ém. Marchal. The four lines showed different levels of tolerance, assessed on the Saari-Prescott scoring scale: TC26 and TC33 proved to be susceptible and TC9 and TC19 resistant. In most of the lines, infection caused changes in the activities of antioxidant enzymes, especially in the case of guaiacol peroxidase. Four peroxidase isoenzymes, which responded differently to powdery mildew infection could be detected by gel electrophoresis. Infection had only a slight effect on the levels of salicylic acid (free and bound forms) in inoculated plants; while the levels of polyamines, especially spermidine and spermine increased after infection. Correlation analysis was also performed to examine how close a relationship exists between the parameters investigated. It was concluded that salicylic acid, polyamines and antioxidant enzymes have an important role in plant responses and defence mechanisms during this biotic stress and that in some cases there were significant relationships between them. However the levels of these compounds either initially or after pathogen inoculation, could not explain the degree of tolerance to powdery mildew in the four wheat lines investigated.
KeywordsAntioxidant enzyme Biotic stress Blumeria graminis tritici Polyamine Salicylic acid Triticum aestivum L.
- PS II
The authors are gratefully indebted to Zsuzsa Kóti and Edit Kövesdi for their technical assistance. Thanks are also due to Barbara Hooper for revising the manuscript linguistically. This work was supported by a grant from the Hungarian National Scientific Research Foundation (OTKA PD83840), which is gratefully acknowledged. Magda Pál is a recipient of the János Bolyai Scholarship.
- Hiraga, S., Ito, H., Yamakawa, H., Ohtsubo, N., Seo, S., Mitsuhara, I., et al. (2000). An HR-induced tobacco peroxidase gene is responsive to spermine, but not to salicylate, methyl jasmonate, and ethephon. Molecular Plant-Microbe Interactions, 13, 210–216.Google Scholar
- Kuznetsov, V. V., & Shevyakova, N. I. (2007). Polyamines and stress tolerance of plants. Plant Stress, 1(1), 50–71.Google Scholar
- Prokopová, J., Mieslerová, B., Hlaváčková, V., Hlavinka, J., Lebeda, A., Nauš, J., & Špundová, M. (2010). Changes in photosynthesis of Lycopersicon spp. plants induced by tomato powdery mildew infection in combination with heat shock pre-treatment. Physiological and Molecular Plant Pathology, 74, 205–213.CrossRefGoogle Scholar
- Saari, E. E., & Prescott, J. M. (1975). A scale for appraising the foliar intensity of wheat diseases. Plant Disease Reporter, 59, 377–380.Google Scholar
- Scott-Craig, J. S., Kerby, K. B., Stein, B. D., & Somerville, S. C. (1995). Expression of an extracellular peroxidase that is induced in barley (Hordeum vulgare) by the powdery mildew pathogen (Erysiphe graminis f. sp. hordei). Physiological and Molecular Plant Pathology, 47, 407–418.CrossRefGoogle Scholar
- Szepesi, Á., Gémes, K., Orosz, G., Pető, A., Takács, Z., Vorák, M., & Tari, I. (2011). Interaction between salicylic acid and polyamines and their possible roles in tomato hardening processes. Acta Biologica Szegediensis, 55, 165–166.Google Scholar
- Talieva, M. N., & Kondrat’eva, V. V. (2002). Influence of exogenous salicylic acid on the level of phytohormones in tissues of Phlox paniculata and Phlox setacea leaves with special reference to resistance against the powdery mildew causative agent Erysiphe cichoracearum DC. f. phlogis Jacz. Biology Bulletin, 29, 551–554.CrossRefGoogle Scholar