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Neofusicoccum parvum associated with fruit rot and shoot blight of peaches in Greece

  • Thomas Thomidis
  • Themis J. Michailides
  • Efstathia Exadaktylou
Article

Abstract

Shoot blights and fruit rots comprise the most serious diseases of peaches in Greece. In this study, the importance of the fungus Neofusicoccum parvum as a casual agent of a fruit rot and shoot blight of peach trees in Greece was investigated. This pathogen was isolated from both immature and mature peach fruit of the cultivar “Catherine” and later on from mature fruit of the peach cultivars “Andross”, “RedHaven”, “Sun Crest” and “Sun Cloud”. In the first year of investigation, N. parvum was found causing preharvest fruit rot and shoot blights of peach trees only at the location “Ammos-Mesi-Meliki Verias” in the prefecture of Imathia (the main peach production area of Greece) at incidences of 30 and 8%, respectively. However, in 2006 N. parvum was isolated from more locations such as Diavatos, Veria, Kopanos and Agia Marina in the prefecture of Imathia, but only at less than 3% of the total surveyed rotted peach fruit and blighted shoots. The pathogen overwintered as sub-epidermal pycnidia in blighted shoots or mummified fruit that remained on peach trees. This study also showed that the optimum temperature for mycelial growth and conidial germination of N. parvum was 25 ºC. Pathogenicity tests using peach fruit showed that isolates of N. parvum and Diplodia seriata (isolated from pistachio grown in the same region) showed no significant differences in their virulence. In laboratory inoculation tests using detached shoots from 25 peach and nectarine cultivars, N. parvum isolates obtained from rotted peaches caused different size cankers on these cultivars. The cultivar Big Top was the most susceptible while the cultivar Maria Bianca the least susceptible.

Keywords

Neofusicoccum parvum Fruit rot Pathogenicity Peaches Shoot blight Virulence 

References

  1. Amponsah, N. T., Jones, E. E., Ridgway, H. J., & Jaspers, M. V. (2008). Production of Botryosphaeria species conidia using grapevine green shoots. New Zealand Plant Protection, 61, 301–305.Google Scholar
  2. Amponsah, N. T., Jones, E. E., Ridgway, H. J., & Jaspers, M. V. (2009). Factors that affect the infection of grapevine tissues with Botryosphaeria species. Phytopathologia Mediterranea, 48, 176–176.Google Scholar
  3. Beckman, T. G., & Reilly, C. C. (2005). Relative susceptibility of peach cultivars to fungal gummosis (Botryosphaeria dothidea). Journal American Pomological Society, 59, 111–116.Google Scholar
  4. Beckman, T. G., Pusey, P. L., & Bertrand, P. F. (2003). Impact of fungal gummosis on peach trees. Hortscience, 38, 1141–1143.Google Scholar
  5. Brown, E. A., & Britton, K. O. (1986). Botryosphaeria diseases of apple and peach in the southeastern United States. Plant Disease, 70, 480–484.CrossRefGoogle Scholar
  6. Caponero, A., Vena, G. M., & Frisullo, S. (1999). Preliminary studies of canker in stone fruits in the Metaponto region. Italus Hortus, 6, 107–108.Google Scholar
  7. Caponero, A., Vena, G. M., Lops, F., Carlucci, A., & Frisullo, S. (2001). A “gummosis disease” of peach trees caused by Botriosphaeria dothidea (Moug.:Fr.) Ces. & De Not. in Italy. Informatore Fitopatologico, 51, 67–70.Google Scholar
  8. Cedelo, L., Mohali, S., & Carrero, C. (1994). First report in Venezuela of Dothiorella dothidea as the cause of brown rot of peach fruits. Fitopatologia Venezolana, 7, 34–36.Google Scholar
  9. Combrink, J. C., Fourie, J. F., & Grobbelaar, C. J. (1984). Botryosphaeria spp. on decayed deciduous fruits in South Africa. Phytophylactica, 16, 251–253.Google Scholar
  10. Crous, W. P., Wingfield, M. J., & Phillips, A. J. L. (2007). Neofusicoccum mediterraneum Crous, M.J. Wingf. & A.J.L. Phillips, sp. nov. Fungal Planet 19.Google Scholar
  11. Damm, U., Crous, P. W., & Fourie, P. H. (2007). Botryosphaeriaceae as potential pathogens of Prunus species in South Africa, with descriptions of Diplodia africana and Lasiodiplodia plurivora sp. nov. Mycologia, 99, 664–680.PubMedCrossRefGoogle Scholar
  12. Espinoza, J. G., Briceno, E. X., Chavez, E. R., Urbez-Torres, J. R., & Latorre, B. A. (2009). Neofusicoccum spp. associated with stem canker and dieback of blueberry in Chile. Plant Disease, 93, 1187–1194.CrossRefGoogle Scholar
  13. Inderbitzin, P., Bostock, R. M., Trouillas, F. P., & Michailides, T. J. (2010) Species of Botryosphaeriaceae from almond in California: Diversity, host range and the new species Neofusicoccum nonquaesitum based on phylogenetic analyses of six loci. Mycologia, 102, 1350–1368.Google Scholar
  14. Laveau, C., Letouze, A., Louvet, G., Bastien, S., & Guerin-Dubrana, L. (2009). Differential aggressiveness of fungi implicated in esca and associated diseases of grapevine in France. Phytopathologie Mediterranea, 48, 32–36.Google Scholar
  15. Ma, Z. H., & Michailides, T. J. (2002). Characterization of Botryosphaeria dothidea isolates collected from pistachio and other plant hosts in California. Phytopathology, 92, 519–526.PubMedCrossRefGoogle Scholar
  16. Ma, Z. H., Boehm, E. W. A., Luo, Y., & Michailides, T. J. (2001). Population structure of Botryosphaeria dothidea from pistachio and other hosts in California. Phytopathology, 91, 665–672.PubMedCrossRefGoogle Scholar
  17. Matheron, M. E., & Mircetich, J. C. (1985). Seasonal variation in susceptibility of Juglans hindsii and Paradox rootstocks of English walnut trees to Phytophthora citricola. Phytopathology, 75, 970–972.CrossRefGoogle Scholar
  18. McDonald, V., Lynch, S., & Eskalen, A. (2009). First report of Neofusicoccum australe, N. luteum, and N. parvum associated with avocado branch canker in california. Plant Disease, 93, 967.CrossRefGoogle Scholar
  19. Michailides, T. J. (1991). Pathogenicity, distribution, sources of inoculum, and infection courts of Botryosphaeria dothidea on pistachio. Phytopathology, 81, 566–573.CrossRefGoogle Scholar
  20. Moral, J., Muñoz-Díez, C., González, N., Trapero, A., & Michailides, T. J. (2010). Characterization and pathogenicity of Botryosphaeriaceae species collected from olive and other hosts in Spain and California. Phytopathology, 100, 1340–1351.Google Scholar
  21. Okie, W. R., & Pusey, P. L. (1996). USDA peach breeding in Georgia: current status and breeding for resistance to Botryosphaeria. Acta Horticulturae, 374, 151–158.Google Scholar
  22. Pantidou, E. M. (1973). List of fungi and hosts in Greece (pp. 223–234). Kifisia, Athens: (Greek), Benaki Phytopathological Institute.Google Scholar
  23. Pavlic, D., Slippers, B., Coutinho, T. A., & Wingfield, M. J. (2009). Molecular and phenotypic characterization of three phylogenetic species discovered within the Neofusicoccum parvum/N-ribis complex. Mycologia, 101, 636–637.PubMedCrossRefGoogle Scholar
  24. Pitt, W. M., Huang, R., Steel, C. C., & Savocchia, S. (2009). Identification, distribution and current taxonomy of Botryosphaeriaceae species associated with grapevine decline in New South Wales and South Australia. Australian Journal of Grape and Wine Research, 16, 258–271.CrossRefGoogle Scholar
  25. Ploetz, C. R., Pérez-Martínez, J. M., Palmateer, J. A., & Tarnowski, T. L. (2009). Neofusicoccum parvum–Induced dieback of Eugenia. Syzygium paniculatum Plant Disease, 93, 804–808.CrossRefGoogle Scholar
  26. Rumbos, I. C., & Phillips, A. J. L. (2005). A new disease of kiwifruit caused by Botryosphaeria parva. South African Journal of Science, 101, XXII.Google Scholar
  27. Slippers, B., Smit, W. A., Crous, P. W., Coutinho, T. A., Wingfield, B. D., & Wingfield, M. J. (2007). Taxonomy, phylogeny and identification of Botryosphaeriaceae associated with pome and stone fruit trees in South Africa and other regions of the world. Plant Pathology, 56, 128–139.CrossRefGoogle Scholar
  28. Slippers, B., Burgess, T., Pavlic, D., Ahumada, R., Maleme, H., Mohali, S., et al. (2009). A diverse assemblage of Botryosphaeriaceae infect Eucalyptus in native and non-native environments. Southern Forests, 71, 101–110.CrossRefGoogle Scholar
  29. Tsopelas, P., Slippers, B., Wingfield, M. J., & Gkonou Zagkou, Z. (2008) Προσβολή δένδρων Sequoiadendron giganteum στην Ελλάδα από το μύκητα Neofusicoccum parvum. 14th National Congress of Hellenic Phytopathological Society, 7–10 October, Argolida Greece (Greek).Google Scholar

Copyright information

© KNPV 2011

Authors and Affiliations

  • Thomas Thomidis
    • 1
  • Themis J. Michailides
    • 2
  • Efstathia Exadaktylou
    • 1
  1. 1.Alexander Technological Education Institute of ThessalonikiSindosGreece
  2. 2.Department of Plant Pathology, Davis, Kearney Agriculture CenterUniversity of CaliforniaParlierUSA

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