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European Journal of Plant Pathology

, Volume 130, Issue 4, pp 541–550 | Cite as

Self-inhibition of spore germination via reactive oxygen in the fungus Cladosporium cucumerinum, causal agent of cucurbit scab

  • Andrey A. Aver’yanov
  • Vera P. Lapikova
  • Tatiana D. Pasechnik
  • Tatiana S. Zakharenkova
  • Con Jacyn Baker
Article

Abstract

Cladosporium cucumerinum spore germination in vitro depended on spore suspension density. Different fungal isolates displayed germination maxima at different spore concentrations. For one isolate, the maximum was observed at the same spore density at both 18 and 25°C, although germination percentage increased slightly at the higher temperature. Diffusates originating in other spore suspensions of the same isolate reduced germination percentage of spores taken at optimal concentration. The least effect occurred in diffusate taken from spores kept at their optimal concentration. Self-suppression of spore germination at unfavourable concentrations was diminished more or less by antioxidants (superoxide dismutase, catalase, mannitol or formate). The same compounds, added to spore diffusates, reduced their fungitoxicities. All diffusates generated superoxide radical (assayed by adrenalin oxidation sensitive to superoxide dismutase). This activity correlated positively with diffusate toxicity. Leaf inoculation of the susceptible cucumber cultivar at 18°C with spore suspensions at extreme densities, at which they germinated poorly in vitro, led to less disease severity then that at optimal density. In contrast, no disease symptoms appeared at 25°C. It is suggested that spores germinating at their extreme concentrations produced reactive oxygen species, suppressing the pathogen; this effect could reduce disease development at low temperatures. At high temperatures, however, this mechanism seems not to work, suggesting that plant infection may be reduced by other disease inhibiting factors.

Keywords

Cucumber Fungitoxicity Spore germination Superoxide radical 

Abbreviations

CAT

catalase

CAT inactiv.

catalase heat-inactivated

O2-

superoxide anion radical

ROS

reactive oxygen species

SOD

superoxide dismutase

Notes

Acknowledgements

The work was partially supported by the grant 2682p of Agricultural Research Service USDA, mediated by International Science and Technology Center.

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Copyright information

© KNPV 2011

Authors and Affiliations

  • Andrey A. Aver’yanov
    • 1
  • Vera P. Lapikova
    • 1
  • Tatiana D. Pasechnik
    • 1
  • Tatiana S. Zakharenkova
    • 1
  • Con Jacyn Baker
    • 2
  1. 1.Research Institute of PhytopathologyRussian Academy of Agricultural SciencesB. VyazemyRussia
  2. 2.Molecular Plant Pathology LaboratoryAgricultural Research Service USDABeltsvilleUSA

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