Tomato leaf deformation virus, a novel begomovirus associated with a severe disease of tomato in Peru
- 344 Downloads
Begomovirus infection was suspected in tomato plants exhibiting symptoms of curling and deformation of leaves observed in a survey conducted in northern and central Peru. Rolling circle amplification and restriction fragment length polymorphism analyses suggested that a begomovirus was present in symptomatic plants. The full-length sequence of a begomovirus DNA component was determined, comprising 2591 nucleotides. Based on its genome organization, we suggest it corresponds to the DNA-A of a New World begomovirus. Less than 89% nucleotide sequence identity to known begomoviruses was found, indicating that it corresponds to an isolate of a distinct begomovirus species for which the name tomato leaf deformation virus (ToLDeV) is proposed. Different stretches of the genomic component have the highest sequence identity with different viruses compatible with a recombinant origin. Sequence segments shared common ancestors with isolates of either soybean blistering mosaic virus, tomato yellow spot virus, or tomato chino La Paz virus. Partial sequence analysis of begomovirus isolates present in symptomatic tomato samples collected in northern and central Peru suggested widespread occurrence of this new begomovirus. This is the first confirmation of a begomovirus infection in tomatoes in Peru.
KeywordsBegomovirus Geminiviridae Recombinant Solanum lycopersicum Tomato leaf deformation virus
This work was supported through grant GEN2006-27770-C2-2 (Ministerio de Educación y Ciencia, Spain, co-financed by FEDER) in the frame of the ERA-NET Plant Genomics project ERA-PG 040B “RCA-GENOMICS” of the European Commission 6th Framework Programme for research and the Trilateral Cooperation GABI-GENOPLANTE-MEC. B. Márquez contract was financed by this same project. E. Fiallo-Olivé was supported by a MAEC-AECID fellowship from Ministerio de Asuntos Exteriores y de Cooperación, Spain. We thank S. García-Andrés for preliminar analyses, and M. V. Martín, R Tovar, and R. Campos for technical assistance.
- Argüello-Astorga, G. R., & Ruiz-Medrano, R. (2001). An iteron-related domain is associated to Motif 1 in the replication proteins of geminiviruses: identification of potential interacting amino acid-base pairs by a comparative approach. Archives of Virology, 146, 1465–1485.PubMedCrossRefGoogle Scholar
- Fiallo-Olivé, E., Martínez-Zubiaur, Y., & Rivera-Bustamante, R. F. (2009). Tomato yellow leaf distortion virus, a new bipartite begomovirus infecting tomato in Cuba. Plant Pathology, 58, 785.Google Scholar
- Galvao, R. M., Mariano, A. C., Luz, D. F., Alfenas, P. F., Andrade, E. C., Zerbini, F. M., et al. (2003). A naturally occurring recombinant DNA-A of a typical bipartite begomovirus does not require the cognate DNA-B to infect Nicotiana benthamiana systemically. The Journal of General Virology, 84, 715–726.PubMedCrossRefGoogle Scholar
- Moriones, E., García-Andrés, S., & Navas-Castillo, J. (2007). Recombination in the TYLCV complex: a mechanism to increase genetic diversity. Implications for plant resistance development. In H. Czosnek (Ed.), Tomato yellow leaf curl virus disease management, molecular biology, breeding for resistance (pp. 119–138). Dordrecht: Springer.CrossRefGoogle Scholar
- Murayama, A., Aragón, L., & Fernández-Northcote, E. N. (2005). Nuevo begomovirus del grupo del nuevo mundo asociado al encrespamiento de la hoja del tomate en la costa del Perú. Fitopatología, 40, 82.Google Scholar
- Rick, C. M. (1976). Tomato. In N. D. Simmonds (Ed.), The evolution of crop plants (pp. 268–273). London and New York: Longman.Google Scholar
- Stanley, J., Bisaro, D. M., Briddon, R. W., Brown, J. K., Fauquet, C. M., Harrison, B. D., et al. (2005). Geminiviridae. In C. M. Fauquet, M. A. Mayo, J. Maniloff, U. Desselberger, & L. A. Ball (Eds.), Virus taxonomy, VIIIth Report of the International Committee on taxonomy of viruses (pp. 301–326). London: Elsevier/Academic Press.Google Scholar