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Tomato leaf deformation virus, a novel begomovirus associated with a severe disease of tomato in Peru

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Abstract

Begomovirus infection was suspected in tomato plants exhibiting symptoms of curling and deformation of leaves observed in a survey conducted in northern and central Peru. Rolling circle amplification and restriction fragment length polymorphism analyses suggested that a begomovirus was present in symptomatic plants. The full-length sequence of a begomovirus DNA component was determined, comprising 2591 nucleotides. Based on its genome organization, we suggest it corresponds to the DNA-A of a New World begomovirus. Less than 89% nucleotide sequence identity to known begomoviruses was found, indicating that it corresponds to an isolate of a distinct begomovirus species for which the name tomato leaf deformation virus (ToLDeV) is proposed. Different stretches of the genomic component have the highest sequence identity with different viruses compatible with a recombinant origin. Sequence segments shared common ancestors with isolates of either soybean blistering mosaic virus, tomato yellow spot virus, or tomato chino La Paz virus. Partial sequence analysis of begomovirus isolates present in symptomatic tomato samples collected in northern and central Peru suggested widespread occurrence of this new begomovirus. This is the first confirmation of a begomovirus infection in tomatoes in Peru.

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Acknowledgements

This work was supported through grant GEN2006-27770-C2-2 (Ministerio de Educación y Ciencia, Spain, co-financed by FEDER) in the frame of the ERA-NET Plant Genomics project ERA-PG 040B “RCA-GENOMICS” of the European Commission 6th Framework Programme for research and the Trilateral Cooperation GABI-GENOPLANTE-MEC. B. Márquez contract was financed by this same project. E. Fiallo-Olivé was supported by a MAEC-AECID fellowship from Ministerio de Asuntos Exteriores y de Cooperación, Spain. We thank S. García-Andrés for preliminar analyses, and M. V. Martín, R Tovar, and R. Campos for technical assistance.

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Authors and Affiliations

  1. Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora”, Universidad de Málaga-Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Estación Experimental “La Mayora”, 29750, Algarrobo-Costa, Málaga, Spain

    Belén Márquez-Martín, Elvira Fiallo-Olivé, Jesús Navas-Castillo & Enrique Moriones

  2. Facultad de Agronomía, Universidad Nacional Agraria La Molina, Av. La Molina s/n, La Molina, Lima, Perú

    Liliana Aragón-Caballero

  3. Centro Nacional de Sanidad Agropecuaria (CENSA), San José de Las Lajas, La Habana, 32700, Cuba

    Elvira Fiallo-Olivé

Authors
  1. Belén Márquez-Martín
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  2. Liliana Aragón-Caballero
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  3. Elvira Fiallo-Olivé
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  4. Jesús Navas-Castillo
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  5. Enrique Moriones
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Correspondence to Enrique Moriones.

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Márquez-Martín, B., Aragón-Caballero, L., Fiallo-Olivé, E. et al. Tomato leaf deformation virus, a novel begomovirus associated with a severe disease of tomato in Peru. Eur J Plant Pathol 129, 1–7 (2011). https://doi.org/10.1007/s10658-010-9699-5

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  • DOI: https://doi.org/10.1007/s10658-010-9699-5

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