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Detection and characterization of benzimidazole resistance of Botrytis cinerea in greenhouse vegetables

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Abstract

From 2003 to 2006, a total of 426 single-conidial isolates of B. cinerea collected from greenhouse vegetables in China were characterized for resistance to benzimidazole fungicides and diethofencarb according to inhibition of mycelial growth. Rapid development of double-resistance to benzimidazoles and diethofencarb was observed. Three types of benzimidazole-resistant isolates, Ben R1, Ben R2 and Ben R3 were detected. A new phenotype, Ben R3, which showed low level of resistance to benzimidazole fungicides and resistance to diethofencarb, was detected with frequencies of 6.8%, 10.0%, 13.2% and 12.4% from 2003 to 2006, respectively. Further studies indicated that Ben R3 was caused by a point mutation from GAG in sensitive(S) isolates to GTG at codon 198 in the β-tubulin gene, predicted to cause a change from glutamic acid to valine. Ben R3 isolates had comparable growth, sporulation and pathogenicity ability as isolates of other phenotypes but were more sensitive at lower temperatures.

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Acknowledgements

This research was partially supported by grant no. Y3080042 of Zhejiang Natural Science Foundation and grant no. 2351000971 of Zhejiang Forest College funding.

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Authors and Affiliations

  1. Department of Crop Protection, Zhejiang Forest College, Hangzhou, 311300, China

    C. Q. Zhang & Y. H. Liu

  2. College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310000, China

    C. Q. Zhang & G. N. Zhu

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  1. C. Q. Zhang
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  2. Y. H. Liu
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  3. G. N. Zhu
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Correspondence to C. Q. Zhang.

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Zhang, C.Q., Liu, Y.H. & Zhu, G.N. Detection and characterization of benzimidazole resistance of Botrytis cinerea in greenhouse vegetables. Eur J Plant Pathol 126, 509–515 (2010). https://doi.org/10.1007/s10658-009-9557-5

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  • DOI: https://doi.org/10.1007/s10658-009-9557-5

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