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European Journal of Plant Pathology

, Volume 124, Issue 1, pp 171–174 | Cite as

Somatic embryogenesis from anthers of the autochthonous Vitis vinifera cv. Domina leads to Arabis mosaic virus-free plants

  • E. G. Borroto-Fernandez
  • T. Sommerbauer
  • E. Popowich
  • A. Schartl
  • M. Laimer
Article

Abstract

Attempts to conserve and utilise autochthonous grapevine germplasm in modern breeding programmes, are sometimes faced with the challenge that virus-free plants of old grapevine varieties and clones are hard to find. From 50 year-old vineyards in Frankonia the Vitis vinifera cv. Domina was selected showing particularly interesting loose-bunch architecture with fewer berries. However this valuable germplasm was carrying an Arabis mosaic virus (ArMV) infection requiring a reliable and effective method to produce healthy mother plants for clonal selection. Somatic embryogenesis was established from anthers as the most promising technical approach. The absence of ArMV in 46 regenerated plant lines was confirmed by ELISA and IC-RT PCR, repeated after different time intervals in vitro and in vivo after acclimatisation, and after one dormancy period under glasshouse conditions. Morphologically, all grapevines appeared true-to-type, and a screening of 20 plants by flow cytometry to determine the ploidy level and to exclude the risk of undesired genetic variability confirmed that all tested plants were diploid. Field evaluations of the initially selected bunch traits are currently underway.

Keywords

Biodiversity Fanleaf disease Vitis vinifera Tissue culture ELISA IC-RT-PCR Ploidy level 

Notes

Acknowledgements

We thank Prof. I. Greilhuber and Dr. E.-M. Temsch (Institute of Botany, University Vienna) for their support in flow cytometry.

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Copyright information

© KNPV 2008

Authors and Affiliations

  • E. G. Borroto-Fernandez
    • 1
  • T. Sommerbauer
    • 1
  • E. Popowich
    • 1
  • A. Schartl
    • 1
  • M. Laimer
    • 1
  1. 1.Plant Biotechnology Unit, IAM, Department BiotechnologyBOKUViennaAustria

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