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Tagging and mapping a second resistance gene for Fusarium wilt race 0 in chickpea

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Abstract

A second gene conferring resistance to the chickpea wilt pathogen, Fusarium oxysporum f. sp ciceris race 0, has been mapped to linkage group 2 (LG2) of the chickpea genetic map. Resistance to race 0 is controlled by two genes which segregate independently; one present in accession JG62 (Foc0 1 /foc0 1 ) and mapping to LG5 and the second present in accession CA2139 (Foc0 2 /foc0 2 ) but remaining unmapped. Both genes separately confer complete resistance to race 0 of the wilt pathogen. Using a Recombinant Inbred Line (RIL) population that segregated for both genes (CA2139 × JG62) and the genotypic information provided by two markers flanking Foc0 1 /foc0 1 ten resistant lines containing the resistant allele Foc0 2 /foc0 2 were selected. Genotypic analysis using these ten resistant lines paired with ten susceptible RILs, selected in the same population, revealed that sequence tagged microsatellite sites (STMS) markers sited on LG2 were strongly associated with Foc0 2 /foc0 2 . Linkage analysis, using data from two mapping populations (CA2139/JG62 and CA2156/JG62), located Foc0 2 /foc0 2 in a region where genes for resistance to wilt races 1, 2, 3, 4 and 5 have previously been reported and which is highly saturated with tightly-linked STMS markers that could be used in marker-assisted selection (MAS).

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References

  • Abbo, S., Molina, C., Jungmann, R., Grusak, M. A., Berkovitch, Z., Kahl, G. (2005). Quantitative trait loci governing carotenoid concentration and weight in seeds of chickpea (Cicer arietinum L.). Theoretical and Applied Genetics, 111, 185–195. doi:10.1007/s00122-005-1930-y.

    Article  PubMed  CAS  Google Scholar 

  • Cho, S., Weidong, C., & Muehlbauer, F. J. (2004). Pathotype-specific genetic factors in chickpea (Cicer arietinum L.) for quantitative resistance to ascochyta blight. Theoretical and Applied Genetics, 109, 733–739. doi:10.1007/s00122-004-1693-x.

    Article  PubMed  Google Scholar 

  • Churchill, G. A., & Doerge, R. W. (1994). Empirical threshold values for quantitative trait mapping. Genetics, 138, 963–971.

    PubMed  CAS  Google Scholar 

  • Cobos, M. J., Fernandez, M. J., Rubio, J., Kharrat, M., Moreno, M. T., Gil, J. (2005). A linkage map of chickpea (Cicer arietinum L.) based on populations from Kabuli X Desi crosses: location of genes for resistance to Fusarium wilt race 0. Theoretical and Applied Genetics, 110, 1347–1353. doi:10.1007/s00122-005-1980-1.

    Article  PubMed  CAS  Google Scholar 

  • Cobos, M. J., Rubio, J., Strange, R. N., Moreno, M. T., Gil, J., & Millan, T. (2006). New QTL for Ascochyta Blight resistance in an RIL population derived from an interspecific cross in chickpea. Euphytica, 149, 105–111. doi:10.1007/s10681-005-9058-3.

    Article  Google Scholar 

  • FAOSTAT data (2007) http://faostat.fao.org/site/567/default.aspx.

  • Halila, M. H., & Strange, R. N. (1996). Identification of the causal agent of wilt of chickpea in Tunisia as Fusarium oxysporum f. sp. ciceri race 0. Phytopathologia Mediterranea, 35, 67–74.

    Google Scholar 

  • Haware, M. P., & Nene, Y. L. (1982). Races of Fusarium oxysporum f. sp. ciceri. Plant Disease, 66, 809–810.

    Google Scholar 

  • Huettel, B., Santra, D., Muehlbauer, F. J., & Kahl, G. (2002). Resistance gene analogues of chickpea (Cicer arietinum L.): isolation, genetic mapping and association with a Fusarium wilt resistance gene cluster. Theoretical and Applied Genetics, 105, 479–490.

    Article  PubMed  CAS  Google Scholar 

  • Iruela, M., Castro, P., Rubio, J., Cubero, J. I., Jacinto, C., Millan, T., et al. (2007). Validation of a QTL for resistance to ascochyta blight linked to resistance to fusarium wilt race 5 in chickpea (Cicer arietinum L.). European Journal of Plant Pathology, 119, 29–37.

    Article  Google Scholar 

  • Jansen, R. C. (1993). Interval mapping of multiple quantitative trait loci. Genetics, 135, 205–211.

    PubMed  CAS  Google Scholar 

  • Jansen, R. C., & Stam, P. (1994). High resolution of quantitative traits into multiple loci via interval mapping. Genetics, 136, 1447–1455.

    PubMed  CAS  Google Scholar 

  • Jiménez-Diaz, R. M., Alcala-Jiménez, A. R., Hervas, A., & Trapero-Casas, J. L. (1993). Pathogenic variability and host resistance in the Fusarium oxysporum. f. sp. Ciceris/ Cicer arietinum pathosystem. In E. Arseniuk, & T. Goral (Eds.), Fusarium Mycotoxins, taxonomy, pathogenicity and host resistance. Proceedings of the 3rd European Seminar. Radzikov, Poland: Plant Breeding and Acclimatization Institute, pp. 87–94.

  • Kosambi, D. D. (1994). The estimation of map distance from recombination values. Annals of Eugenics, 12, 172–175.

    Google Scholar 

  • Lander, E. S., & Botstein, D. (1989). Mapping Mendelian factors underlying quantitative traits using RFLP linkage map. Genetics, 121, 185–199.

    PubMed  CAS  Google Scholar 

  • Millan, T., Clarke, H. J., Siddique-Kadambot, H. M., Buhariwalla, H. K., Gaur, P. M., Kumar, J., et al. (2006). Chickpea molecular breeding: new tools and concepts. Euphytica, 147, 81–103.

    Article  Google Scholar 

  • Parniske, M., Hammond-Kosack, K. E., Golstein, C., Thomas, C. M., Jones, D. A., Harrison, K., et al. (1997). Novel disease resistance specificities result from sequence exchange between tandemly repeated genes at the Cf-4/9 locus of tomato. Cell, 91, 821–832.

    Article  PubMed  CAS  Google Scholar 

  • Pfaff, T., & Kahl, G. (2003). Mapping of gene-specific markers on the genetic map of chickpea (Cicer arietinum L.). Molecular Genetics and Genomics, 269, 243–251.

    PubMed  CAS  Google Scholar 

  • Ratnaparkhe, M. B., Santra, D. K., Tullu, A., & Muehlbauer, F. J. (1998a). Inheritance of inter-simple-sequence-repeat polymorphisms and linkage with a Fusarium wilt resistance gene in chickpea. Theoretical and Applied Genetics, 96, 348–353.

    Article  CAS  Google Scholar 

  • Ratnaparkhe, M. B., Tekeoglu, M., & Muehlbauer, F. J. (1998b). Inter-simple-sequence-repeat (ISSR) polymorphisms are useful for finding markers associated with disease resistance gene clusters. Theoretical and Applied Genetics, 97, 515–519.

    Article  CAS  Google Scholar 

  • Rubio, J., Hajj-Moussa, A. E., Kharrat, M., Moreno, M. T., Millan, T., & Gil, J. (2003). Two genes and linked RAPD markers involved in resistance to Fusarium oxysporum f. sp. ciceris race 0 in chickpea. Plant Breeding, 122, 188–191.

    Article  CAS  Google Scholar 

  • Sharma, K. D., & Muehlbauer, F. J. (2007). Fusarium wilt of chickpea: physiological specialization, genetics of resistance and resistance gene tagging. Euphytica, 157, 1–14.

    Article  CAS  Google Scholar 

  • Sharma, K. D., Winter, P., Kahl, G., & Muehlbauer, F. J. (2004). Molecular mapping of Fusarium oxysporum f. sp. ciceris race 3 resistance gene in chickpea. Theoretical and Applied Genetics, 108, 1243–1248.

    Article  PubMed  CAS  Google Scholar 

  • Sharma, K. D., Chen, W., & Muehlbauer, F. J. (2005). Genetics of chickpea resistance to five races of Fusarium wilt and a concise set of race differentials for Fusarium oxysporum f. sp.ciceris. Plant Disease, 89, 385–390.

    Article  Google Scholar 

  • Song, W. Y., Pi, L. Y., Wang, G. L., Gardner, J., Holsten, T., & Ronald, P. C. (1997). Evolution of the rice Xa21 disease resistance gene family. The Plant Cell, 9, 1279–1287.

    Article  PubMed  CAS  Google Scholar 

  • Tekeoglu, M., Tullu, A., Kaiser, W. J., & Muehlbauer, F. J. (2000). Inheritance and linkage of two genes that confer resistance to Fusarium wilt in chickpea. Crop Science, 40, 1247–1251.

    CAS  Google Scholar 

  • Tullu, A., Muehlbauer, F. J., Kaiser, W. J., Simon, C. J., Mayer, M. S., Kumar, J., & Kraft, J. M. (1998). Inheritance and linkage of a gene for resistance to race 4 of Fusarium wilt and RAPD markers in chickpea. Euphytica, 102, 227–232.

    Article  CAS  Google Scholar 

  • Udupa, S. M., & Baum, M. (2003). Genetic dissection of pathotype-specific resistance to ascochyta blight disease in chickpea (Cicer arietinum L.) using microsatellite markers. Theoretical and Applied Genetics, 106, 1196–1202.

    PubMed  CAS  Google Scholar 

  • Van Ooijen, J. W. (1992). Accuracy of mapping quantitative trait loci in autogamous species. Theoretical and Applied Genetics, 84, 803–811.

    Article  Google Scholar 

  • Van Ooijen, J. W. (2004). MAPQTL 5, software for the mapping of quantitative trait loci in experimental populations. Plant Res. Int., Wageningen.

  • Van Ooijen, J. W., & Voorrips, R. E. (2001). Joinmap 3.0, Software for the calculation of genetic maps. Plant Res. Int., Wageningen.

  • Winter, P., Pfaff, T., Udupa, S. M., Huttel, B., Sharma, P. C., Sahi, S., et al. (1999). Characterization and mapping of sequence-tagged microsatellite sites in the chickpea (Cicer arietinum L.) genome. Molecular Genetics and Genomics, 262, 90–101.

    CAS  Google Scholar 

  • Winter, P., Benko-Iseppon, A. M., Huttel, B., Ratnaparkhe, M., Tullu, A., Sonnante, G., et al. (2000). A linkage map of the chickpea (Cicer arietinum L.) genome based on the recombinant inbred lines from C. arietinum × C. reticulatum cross: localization of resistance genes for Fusarium race 4 and 5. Theoretical and Applied Genetics, 101, 1155–1163.

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work has been supported by the Tunisian–Spanish cooperation project: AECI, contract no. 11/04/P/T, and the Spanish project: INIA, contract no. RTA04-067. Authors are indebted to Dr Richard Strange from Birkbeck College, University of London for his valuable comments and suggestions.

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Authors and Affiliations

  1. Field Crops Laboratory, INRAT, Rue Hedi Karray, 2080, Ariana, Tunisia

    I. Halila & M. Kharrat

  2. Area de Mejora y Biotecnologia, IFAPA, Aptdo. 3092, 14080, Cordoba, Spain

    M. J. Cobos & J. Rubio

  3. Dpto. de Genetica, Universidad de Cordoba, Campus de Rabanales Edif. C-5, 14071, Cordoba, Spain

    T. Millán & J. Gil

  4. Molecular Genetics, Immunology and Biotechnology Laboratory, FST, 1060, Tunis, Tunisia

    M. Marrakchi

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  1. I. Halila
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  2. M. J. Cobos
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  3. J. Rubio
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  4. T. Millán
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  5. M. Kharrat
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  6. M. Marrakchi
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  7. J. Gil
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Correspondence to J. Gil.

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Halila, I., Cobos, M.J., Rubio, J. et al. Tagging and mapping a second resistance gene for Fusarium wilt race 0 in chickpea. Eur J Plant Pathol 124, 87–92 (2009). https://doi.org/10.1007/s10658-008-9395-x

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  • DOI: https://doi.org/10.1007/s10658-008-9395-x

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