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European Journal of Plant Pathology

, Volume 123, Issue 2, pp 241–246 | Cite as

Suppression of Papaya ringspot virus infection in Carica papaya with CAP-34, a systemic antiviral resistance inducing protein from Clerodendrum aculeatum

  • Aparana Srivastava
  • Sonali Trivedi
  • Sunil Kumar Krishna
  • H. N. Verma
  • Vivek Prasad
Article

Abstract

CAP-34, a protein from Clerodendrum aculeatum inducing systemic antiviral resistance was evaluated for control of Papaya ringspot virus (PRSV) infection in Carica papaya. In control plants (treated with CAP-34 extraction buffer) systemic mosaic became visible around 20 days that intensified up to 30 days in 56% plants. During this period, CAP-34-treated papaya did not show any symptoms. Between 30 and 60 days, 95% control plants exhibited symptoms ranging from mosaic to filiformy. In the treated set during the same period, symptoms appeared in only 10% plants, but were restricted to mild mosaic. Presence of PRSV was determined in induced-resistant papaya at the respective observation times by bioassay, plate ELISA, immunoblot and RT-PCR. Back-inoculation with sap from inoculated resistant plants onto Chenopodium quinoa did not show presence of virus. The difference between control and treated sets was also evident in plate-ELISA and immunoblot using antiserum raised against PRSV. PRSV RNA was not detectable in treated plants that did not show symptoms by RT-PCR. Control plants at the same time showed a high intensity band similar to the positive control. We therefore suggest that the absence/delayed appearance of symptoms in treated plants could be due to suppressed virus replication.

Keywords

Induced systemic resistance Inhibition of virus replication RT-PCR Immunoblot ELISA 

Notes

Acknowledgements

The authors gratefully acknowledge Dr R K Jain, Indian Agricultural Research Institute for the gift of the PRSV antiserum. Financial assistance from the Department of Science and Technology, New Delhi, is also gratefully acknowledged.

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Copyright information

© KNPV 2008

Authors and Affiliations

  • Aparana Srivastava
    • 1
  • Sonali Trivedi
    • 1
  • Sunil Kumar Krishna
    • 1
  • H. N. Verma
    • 2
  • Vivek Prasad
    • 1
  1. 1.Molecular Plant Virology Lab, Department of BotanyUniversity of LucknowLucknowIndia
  2. 2.Seedling Academy of Design, Technology and ManagementJaipurIndia

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