European Journal of Plant Pathology

, Volume 122, Issue 2, pp 315–319 | Cite as

Monitoring changing virulence patterns of Uromyces appendiculatus in the resistant pinto bean cultivar Olathe by rep-PCR

  • Angela T. Alleyne
  • Jim R. Steadman
  • Kent M. Eskridge


Corresponding molecular markers associated with avirulence or virulence genes in the bean rust pathogen are currently unknown, although host resistance genes have been linked to molecular markers in bean. The changing virulence patterns in pathotypes of Uromyces appendiculatus collected over a 14-year period after the release of the Ur-6 resistance gene on the USA high plains were therefore analyzed using rep-PCR molecular markers. Isolates from two neighbouring pathotype groups from Colorado and Nebraska were screened using the rep-PCR primer Box-AIR. The PCR fragment Box 475 was cloned and sequenced and a specific primer set ATA-2 was designed. This primer yielded 10 polymorphic products which allowed separation of these isolates into two distinct groups and will be useful for future analysis of the population genetics of this organism.


Bean rust Virulence Epidemiology 



A contribution of the University of Nebraska, Agricultural Division, Lincoln, NE., 68583, Journal series No. 14210. This work was funded by a postdoctoral fellowship at the University of Nebraska-Lincoln, Department of Plant Pathology and also support from the Bean/Cowpea CRSP (USAID contract No. DAN-1310-G-SS-6008-00).


  1. Altschul, S. F., Madden, T. L., Schäffer, A. A., Zhang, J., Zhang, Z., Miller, W., et al. (1997). Gapped BLAST and PSI-BLAST: A new generation of protein data-base search programs. Nucleic Acid Research, 25, 3389–3402.CrossRefGoogle Scholar
  2. Araya, C. M., Alleyne, A. T., & Steadman, J. R. (2004). Phenotypic and genotypic characterization of Uromyces appendiculatus from Phaseolus vulgaris in the Americas. Plant Disease, 88(8), 830–836.CrossRefGoogle Scholar
  3. Gross, P. L., & Venette, J. R. (2002). Bean rust races in North Dakota. Bean Improvement Cooperative (BIC) Report, 45, 106–107.Google Scholar
  4. Linde, D. C., Groth, J. V., & Roelfs, A. P. (1990). Comparison of isozyme and virulence diversity patterns in the bean rust fungus Uromyces appendiculatus. Phytopathology, 80, 141–147.CrossRefGoogle Scholar
  5. Louws, F. J., Schneider, M., & DeBruijn, F. J. (1996). Assessing genomic diversity of microbes using repetitive-sequence based PCR (rep-PCR). In G. Toranzos (Ed.) Nucleic acid amplification methods of environmental samples (pp. 63–94). Lancaster: Technomic Publishing Co..Google Scholar
  6. McCain, J. W., Ozmon, E. A., & Groth, J. V. (1990). Virulence frequency in the bean rust fungus: comparison of phenotypic vs. genotypic polymorphism. Plant Disease, 74, 496–501.CrossRefGoogle Scholar
  7. Miklas, P. N., Jinguo, H., Grunwald, J., & Larsen, K. M. (2006). Potential application of TRAP (Targeted region amplified polymorphism) markers of mapping and tagging disease resistance traits in common bean. Crop Science, 46, 910–916.CrossRefGoogle Scholar
  8. Mmbaga, M. T., Steadman, J. R., & Eskridge, K. M. (1996). Virulence patterns of Uromyces appendiculatus from different geographical areas and implications for finding durable resistance to rust in common bean. Phytopathology, 144, 533–541.CrossRefGoogle Scholar
  9. Park, S. O., Coyne, D. P., Stedman, J. R., Crosby, K. M., & Brick, M. A. (2004). RAPD and SCAR makers linked to the Ur-6 Andean gene controlling specific rush resistance in common bean. Crop Science, 44, 1799–1807.CrossRefGoogle Scholar
  10. Pastor-Corrales, M. A., & Stavely, J. R. (2002). Using races of the common bean rust pathogen to detect resistance genes in Phaseolus vulgaris. Bean Improvement Cooperative (BIC) Report, 45, 78–79.Google Scholar
  11. Stavely, J. R. (1984). Pathogenic specialization in Uromyces phaseoli in the United States and rust resistance in beans. Plant Disease, 68, 95–99.CrossRefGoogle Scholar
  12. Stavely, J. R. (2000). Pyramiding rust and viral resistance genes using traditional and marker assistance techniques in common bean. Bean Improvement Cooperative Report, 43, 1–4.Google Scholar
  13. Stavely, J. R., Steadman, J. R., & McMillan, R. T. (1989). New pathogenic variability in Uromyces appendiculatus in North America. Plant Disease, 73, 428–432.CrossRefGoogle Scholar
  14. Steadman, J. R., O’Keefe, D., Kerr, E. D., & Schwartz, H. F. (1996). Pathogen variability in bean rust implications for release of resistant cultivars on the US high plains. Bean Improvement Cooperative Report, 39, 148–149.Google Scholar

Copyright information

© KNPV 2008

Authors and Affiliations

  • Angela T. Alleyne
    • 1
  • Jim R. Steadman
    • 2
  • Kent M. Eskridge
    • 3
  1. 1.Department of BiologyEdward Waters CollegeJacksonvilleUSA
  2. 2.Department of Plant PathologyUniversity of Nebraska–LincolnLincolnUSA
  3. 3.Department of BiometryUniversity of Nebraska–LincolnLincolnUSA

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