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Fusaproliferin effects on the photosystem in the cells of maize seedling leaves

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Abstract

The possible role of the fusariotoxin, fusaproliferin in plant pathology was investigated with respect to cell membrane potential. Electron microscopy was used to study both the early effect of fusaproliferin on the host’s plasma membrane and ultrastructure responses in the cells of maize leaves. The seedlings of resistant (Lucia) and susceptible (Pavla) to the fusaproliferin maize cultivars were grown in the presence of fusaproliferin at different concentrations, namely 5 and 35 μg ml−1, respectively, and electrophysiological measurements were compared with those obtained using two different toxic compounds, namely fusicoccin and 3-3(3,4 dichlorophenyl)-1,1-dimethylurea (DCMU). It was observed that only the higher concentration of fusaproliferin induced the onset of visible symptoms on the leaves. Comparing the effect of fusaproliferin to that of fusicoccin and DCMU at the higher toxin concentration, it was observed that functional differences in membrane potential induced severe damage to the mesophyll and outer chloroplast membrane; the extent of changes in electrophysiology and ultrastructure disturbances depended on the toxin concentration and was greater in the susceptible cv. Pavla. Results indicated that fusaproliferin could be involved in Fusarium pathogenesis either as a virulence factor or by enhancing the activity of other toxins that might be concomitantly present in infected plants.

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Acknowledgement

This work was supported by Slovak Grant Agency VEGA, Project No.2/3051/25, 2/6056/26 and SAS/CNR cooperation.

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Authors and Affiliations

  1. Dipartimento di Scienza degli Alimenti, Università degli Studi di Napoli “Federico II”, Via Universitá 100, 80055, Portici, Napoli, Italy

    Antonello Santini & Alberto Ritieni

  2. Institute of Botany, Slovak Academy of Sciences, Dúbravská cesta 14, 84523, Bratislava, Slovakia

    Antonia Šrobárová, Ján Pavlovkin & Milada Čiamporová

Authors
  1. Antonello Santini
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  2. Antonia Šrobárová
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  3. Ján Pavlovkin
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  4. Milada Čiamporová
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  5. Alberto Ritieni
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Correspondence to Antonello Santini.

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Santini, A., Šrobárová, A., Pavlovkin, J. et al. Fusaproliferin effects on the photosystem in the cells of maize seedling leaves. Eur J Plant Pathol 120, 363–371 (2008). https://doi.org/10.1007/s10658-007-9226-5

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  • DOI: https://doi.org/10.1007/s10658-007-9226-5

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